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New immunotherapy for prevention of liver metastasis from colorectal cancer after surgery.

Research Project

Project/Area Number 02670579
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Digestive surgery
Research InstitutionYamaguchi University

Principal Investigator

OKA Masaaki  Yamaguchi Univ. of Med. Assistant Professor, 医学部, 講師 (70144946)

Co-Investigator(Kenkyū-buntansha) HAZAMA Shoichi  Yamaguchi Univ. Hospital Resident, 医学部附属病院, 医員
UCHIYAMA Tetsuji  Yamaguchi Univ. of Med. Assistant, 医学部, 助手 (90136177)
SUZUKI Takashi  Yamaguchi Univ. of Med. Professor, 医学部, 教授 (20026834)
光永 斎  山口大学, 医学部・附属病院, 助手 (70174076)
Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1992: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1991: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1990: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsColorectal cancer / Liver metastasis / interleukin-2 / prostaglandin E2 / 免疫療法 / IL‐2 / CTL / ラット転移性肝癌モデル / ILー2 / 門脈内持続投与 / NK活性 / LAK活性 / 術後免疫能
Research Abstract

(Experimental study) Donryu rats were inoculated with AH-130 tumor cells on day 0 via the portal vein, and 2 days later, IL-2 was administered continuously via the portal vein (P group), or into the subcutaneous space (S group). Control rats were received saline alone (C group). On day 8, the incidence of liver metastasis and cytotoxicities (NK, LAK and anti-AH-130 activity) of liver non-parenchymal cells (NPC), In P groups, hepatic metastasis were significantly reduced, and NK, LAK and anti-AH-130 activities of NPC were significantly augmented compared with C group. However, these effects were not observed in S group. LAK cells were induced from splenocytes and lymphocytes of the lymph nodes using ICR mice, cultured with IL-2. LAK cells cultured for 7 days showed about 70% of LAK activity.
(Clinical study) We determined prostaglandin E2 (PGE2) (n=23) levels and lymphocyte subsets (n=10) in portal venous drainage of colorectal cancer. The PGE2 levels in the portal vein were significantly higher than those in the artery. Four of nine patients with more than 100pg/ml of portal PGE2 developed liver metastasis within two years following surgery. For prevention of recurrence in the liver, four patients with liver metastasis from colorectal cancer received intrahepatic combined immunotherapy using IL-2, OK-432, adriamycin, cyclophosphamide, and famotidine after hepatectomy. All patients are still alive more than 2 years. In conclusion, intrahepatic combined immunotherapy may be useful for prevention of liver metastasis from colorectal cancer. PGE2 levels in the portal vein may be anew predictor for liver recurrence from colorectal cancer.

Report

(4 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Masaaki Oka et al: "Prostaglandin E_2 levels and lymphocyte subsets in portal venous drainage of colorectal cancer." The American Journal of Surgery (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 岡 正朗 他: "大腸癌肝転移に対して肝切除と術後肝動注多剤免疫化学療法を施行した4例の検討" 日本消化器外科学会雑誌.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 岡 正朗 他: "大腸癌患者における門脈内prostaglandin E_2 (PGE_2)とリンパ球サブセット" Biotherapy.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 岡 正朗 他: "手術患者における術後免疫能と術前recombinant interleukin-2持続投与の影響" Biotherapy. 5. 942-943 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Masaaki Oka et al: "Prostaglandin E2 levels and lymphocyte subsets in portal venous drainage of colorectal cancer" The American Journal of Surgery.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Masaaki Oka et al.: "Case study of intrahepatic combined immunochemotherapy after hepatic resection for four patients with liver metastasis from colorectal cancer." J Jpn Gastroenterol Surg.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Masaaki Oka et al.: "Prostaglandin E2 levels and lymphocyte subset in the draining portal vein of colorectal cancer." Biotherapy.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Masaaki Oka: "Influence of surgical stress on immunity and effects of preoperative administration of interleukin-2 on surgically induced immunosuppression." Biotherapy. 5. 942-943 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Masaaki Oka et al: "Prostaglandin E_2 levels and lymphocyte subsets in portal venous drainage of colorectal cancer." The American Journal of Surgery (in press).

    • Related Report
      1992 Annual Research Report
  • [Publications] 岡 正朗 他: "大腸癌肝転移に対して肝切除と術後肝動注多剤免疫化学療法を施行した4例の検討" 日本消化器外科学会雑誌.

    • Related Report
      1992 Annual Research Report
  • [Publications] 岡 正朗 他: "大腸癌患者における門脈内prostaglandin E_2(PGE_2)とリンパ球サブセット" Biotherapy.

    • Related Report
      1992 Annual Research Report
  • [Publications] 岡 正朗 他: "手術患者における術後免疫能と術前recombinant interleukin-2持続投与の影響" Biotherapy. 5. 942-943 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] 硲 彰一: "肝細胞癌に対するCombination Immunotherapyの有用性について" 癌と化学療法. 17. 1638-1642 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 岡 正朗: "手術侵襲と免疫能ー開胸術と開腹術における術後免疫能の比較ー" 腫瘍と感染. 3. 831-835 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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