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^<31>P NMR and EEG study of transient global ischemia in developing dog brain compared with adult dog

Research Project

Project/Area Number 02670629
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Cerebral neurosurgery
Research InstitutionKobe University

Principal Investigator

FUJITA Katsuzo  Kobe Univ. Sch. Med., Associate Professor, 医学部, 助教授 (60093530)

Co-Investigator(Kenkyū-buntansha) OI Sizuo  Kobe Univ. Hos, Assistant Professor, 医学部附属病院, 講師 (30194062)
TAMAKI Norihiko  Kobe Univ. Sch. Med., Professor, 医学部, 教授 (10030941)
松本 悟  神戸大学, 医学部, 教授 (10030850)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1991: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1990: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsCerebral Ischemia / Cerebral Metabolism / Magnetic Resonance Spectroscopy / Electroencephaloscopy / 脳虚血 / 脳代謝 / MR Spectroscopy / NMRーSpectroscopy / ischemia / cerebral metabolism / immature brain
Research Abstract

The reversibility and plasticity of brain energy metabolism and function after ischemic insult is related to the degree of neuronal maturation. A quantitative estimation of the capability to recover from brain damage, however, is difficult, because the degree of metabolic derangement differs markedly between immature and adult animals. Using a global ischemia model, and giving the same degree of ischemic insult, we compared the recovery time course among infant, young and adult dog groups.
Methods
Twelve mongrel dogs from three age groups were used for the study Group A, 4 adult(one year) ; Group B, 4 young adult dogs(three months) ; Group C, 4 infant dogs(38 days). Animals were tracheotomized under Innovar anesthesia, and ventilated with 30% oxygen.
Magnetic resonance spectra were obtained using a Phospho-Energetics 250-80 NMR spectrometer. Simultaneously, the parietal cortical EEG was recorded. Global ischemia was produced by occlusion of both the innominate and left subclavian arteries … More . Hypotension(Systolic blood pressure less than 100 mm Hg)was produced by withdrawal of blood.
Results
*NMR spectra ; During global ischemia, the beta-ATP ratio, ischemia value to control value, decreased to 0.31<plus-minus>0.03 in Group A, 0.38<plus-minus>0.03 in Group B and 0.43<plus-minus>0.05 in Group C. There were no significant differences among the three age groups. Four hours after recirculation, the -ATP ratio of Groups B and C returned to 0.90<plus-minus>0.01 and 0.97<plus-minus>0.04 respectively, while in Group A the ratio returned to 0.71<plus-minus>0.05. The level of ATP recovery in Group B and C significantly larger when compared to Group A(P<0.00
On the other hand, the recovery time course of PCr/Pi showed no differences among the three age groups. (Fig. 2)
*EEG ; Group A : With the onset of ischemia, almost all(alpha waves(813Hz)disappeared and there was no apparent recovery. (Fig. 3) Group B : The recovery of EEG was incomplete and recovery was delayed when compared to3 : LP spectral changes. Group C : The EEG recovered to near the control level, however, recovery was delayed remarkably when compared with3lp NMR soectral changes. (Fig. 4)
Discussion
This 31P NMR study monitored ATP depletion during global ischemia and evaluated the threshold level for recovery in dogs of various ages. An ATP reduction to 30-40% from the normal level sustained for 15 minutes results in nearly full recovery in infant dogs, while adult animals showed irreversible brain damage. In the case of infant, the EEG recovered almost completely. Only time delay of EEG recovery was seen. Less

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] K.Fujita,X.hinn,N.Tamaki.: "Assessment of the cerebral vasospasm from the chenges of CO_2 reactivity and vascular resistance by TCD" Newrosonology. 4. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K.Fujita,X.hinn,N.Ehara K,N.Tamaki: "Quautitatine meesurement of besal cerebral orterial blood flow by TCD in cerebral arteriovenons malformations." Newrosonology. 4. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K. Fujita, X. Kinn, N. Tamaki: "Assessment of the cerebral vasospasm from the changes of CO_2 reactivity and vasalar reecitance by TCD" Newrosonology. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K. Fujita, X. Kinn, Ehara K, N. Tamaki: "Quantitative measurement of basal cerebral artical blood flow by TCD in cerebral artioveroos malformations." Newrosonology. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 藤田 勝三,玉木 紀彦,松本 悟: "頚動脈海綿静脈洞瘻に対するelectrothrombosriによる治療" 脳卒中の外科. 18. 337-342 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 藤田 勝三,白口 隆行,林 賢浜,朝田 一雄,江原 一雅,玉木 紀彦: "重症クモ膜下出血後のsymptomatic Vhsospasmに対するticlopidine,Albuomn,nicardipineの併用療法の有効性について" 脳卒中の外科. 19. 247-251 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 藤田 勝三,白口 隆行,鈴木 寿彦,増村 道雄,朝田 一雄,江原 一雅,玉木 紀彦: "脳動脈瘤のmck clippong後に発生した窩通動脈閉塞症例の検討" 脳卒中の外科. 19. 624-630 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 藤田 勝三,玉木 紀彦: "脳血管障害と凝固能異常" 臨床病理. 39. 729-735 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 藤田 勝三,白口 隆行,江原 一雅,朝田 一雄,玉木 紀彦: "高令者重症くも膜下出血症例に対する療法方針" Geriatinc Neuroswgevy. 4. 112-116 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 藤田 勝三: "頚動脈海綿静脈洞瘻に対するelectro thrombosisによる治療" 脳卒中の外科. 18. 337-342 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 藤田 勝三: "重症くも膜下出血後のSymptomatic Vasospasmに対するTANーtherapyの有効性について" 脳血管攣縮. 5. 219-228 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 藤田 勝三: "抗血小板剤,線溶凝固" 脳血管攣縮. 5. 28-33 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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