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FLOWCYTOMETRIC ANALYSES OF EXPRESSIONS OF GLYCOPROTEIN ANTIGEN AND EGF-RECEPTORS, AND DNA DISTRIBUTION IN BRAIN TUMORS

Research Project

Project/Area Number 02670640
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Cerebral neurosurgery
Research InstitutionKANSAI MEDICAL UNIVERSITY

Principal Investigator

KAWAMOTO Keiji  KANSAI MEDICAL UNIVERSITY NEUROSURGERY ASSISTANT PROFESSOR, 医学部(脳神経外科), 講師 (70077741)

Co-Investigator(Kenkyū-buntansha) SAKAI Nobuyuki  KANSAI MEDICAL UNIVERSITY NEUROSURGERY ASSISTANT, 医学部(脳神経外科), 助手 (30225768)
NUMA Yoshihiro  KANSAI MEDICAL UNIVERSITY NEUROSURGERY ASSISTANT, 医学部(脳神経外科), 助手 (40208278)
KAWAKAMI Katsuhiro  KANSAI MEDICAL UNIVERSITY NEUROSURGERY ASSISTANT PROFESSOR, 医学部(脳神経外科), 講師 (40186060)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1991: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1990: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsbrain tumor / glycoprotein / EGF / EGF-receptor / flow cytometry / oncogene, / DNA-histogram / EGFーR / FcM / DNA / Glyco protein / flow cytometny / brain tuwor
Research Abstract

1)Flowcytometric analysis of the cell cycle in brain tumors:
Flow cytometry (FCM) has been an effective method to grasp biological aspects of brain tumors. Of brain tumors, we have reported how primitive ectodermal tumor cells proliferate with the advance of passages of cell culture, and have proposed a new classification of ploidy patterns.
2)Flowcytometric evaluation of action mechanism of antineoplastic agents:
The effects of cisplatin and carboplatin appear at the S and G_2M phase. NK cells sorted with sterile technique have antineoplastic effects and the effects could be enhanced by addition of interleukin-1. The effects of lymphokine-activated killer cells were confirmed in vitro and the action mechanism was clarified.
3)Expression of oncogene products in brain tumors:
In established brain tumor cell strains, immunohistochemical and flowcytometric methods were useful for evaluation of the positivity of c-myc products expression.
4)Expression of glycoprotein antigen in brain tumors:
The subjects included an established KB cell line, brain tumors, lung cancers, uterus carcinomas, cancers in the head and neck. Of brain tumors, the expression was highly positive in metastatic tumors and epithelial cancers. FCM was useful in evaluating the expression.
5)Expressions of EGF and EGF-R:
The expressions differed according to the method of sample fixation. EGF expressed in various brain tumors, suggesting a correlation with malignancy. FCM was a simple and useful method for such study.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Imahori,T.Kawamoto K,Matsumura: "Flow cytometric analysis of suti-Sialyl SSEA-1 expnession in clinical waterials" J Jpn Soc.Cancer Thev. 27. 290- (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 今堀 巧 河本 圭司 坂井 信義 沼 義博 松村 浩: "フローサイトメトリーを用いてEGFとEGF-Rの発現からみた細胞増殖と制御の検討" 神経免疫研究. 4. 229-234 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Numa Y,Kawamoto K.Sakai N Matsumura H: "Flow cytometric analysis of antineu plastic effets of lnterfevon α,β γ lakeled with floureceim isthio cyauate on cultured brain tumsr" J Neuroouology. 11. 225-234 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 喜多川 直子,植村 興 河本 圭司: "LAK細胞の作成と抗腫瘍効果" Ducologyの進歩. 1. 70-75 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kawamoto.K Fujiwara.Numa.Matsumura: "Antineoplastic effets of uatuvel killer cells sorted with flow cytometry on drain tumovs" Human Cell. 4. 157-164 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 坂井 信幸,河本 圭司 松村 浩: "フローサイトメトリーを用いた悪性ブリオーマにおけるL-wyc遺伝子産物とGFAPの発現の関係の検討" cytonitry Receareh. 1. 85-89 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kawamoto K: "Cell Kiuctics of cultuved main tuwer cells Flow cytometric and image analysis for cliuical applications" Elsevier Science Publications, 260 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 河本 圭司: "脳腫瘍の免疫と分子生物学 予定" 金芳堂, 350 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Imahori T, Kawamoto K, Matsumura H: "Flow cytometric analysis anti-sialyl SSEA-1 expression in clinical materials." J Jpn Soc Cancer Ther. 27. 290 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Imahori T, Kawamoto K, Sakai N, Matsumura H: "Flow cytometric analysis of cell proliferation and control based on expressions of EGF and EGF-R." Neuroimmunol Res. 4. 229-234 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Numa Y, Kawamoto K, Sakai N, Matsumura H: "Flow cytometric analysis of antineoplastic effects of interferon-alpha, beta, gamma labeled with fluorescein isothiocyanate on cultured brain tumor." J Neuro-Oncol. 11. 225-234 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hasegawa N, Uemura T, Kawamoto K: "Preparation of LAK cells and their antineoplastic effects." Advances in Oncology. 1. 70-75 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kawamoto K, Fujiwara H, Numa Y, Matsumura H: "Antineoplastic effects of natural killer cells sorted with flow cytometry on brain tumors." Human Cell. 4. 157-164 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sakai N, Kawamoto K, Matsumura H: "Flow cytometric study on the expressions of c-myc oncogene products and GFAP in malignant gliomas." Cytometry Res. 1. 85-89 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kawamoto K: "Cell kinetics of cultured brain tumor cells" In: Flow Cytometric and Image Analysis for Clinical Applications, Elsevier Science. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kawamoto K: "Immunology and Molecular Biology in Brain Tumor" Kinpodo. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kawamoto.k.et al: "Siguifilant of flow cytometric measurement of DNA-Index in gliomas" Brain Tumor Pathol. 8. 17-11 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kawamoto,K.et al: "Immuno histochemical,electron microscopic,and flow cytometic studies in three cases of PNET" Brain Tumor Pathol. 8. 137-141 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kawamoto K.et al: "Autoneoplastic effects of natural killer cells sorted with flow cytometny an brain tumors" Human Cell. 4. 157-164 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 坂井 信幸,河本 圭司: "フロ-サイトメトリ-を用いた電性グリオ-マにおけるCーmyc遺伝子産物とGFAPの発現の検討" Cytometry Research. 1. 85-89 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 今堀 巧,河本 圭司他: "フロ-サイトメトリ-を用いてEGFとEGRーRの発現からみた細胞運動と制御の検討" 神経免疫研究. 4. 229-234 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Imahori,T.Kawamoto K.: "Flow cytometic analysis of anti-Sialye SSEA-1 expression in clinical metemals." J.Jpn Soc.Cancer Ther.27. 180 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 河本 圭司,坂井 信幸,今堀 巧,松村 浩: "新しいDNA ptody分類の提案" フロ-サイトメトリ-. 10. 124-131 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 今堀 巧,河本 圭司,坂井 信幸,沼 義博,松村 清: "フロ-サイトメトリ-を用いた脳腫瘍の糖鎖抗原発現の基礎的検討" 脳神経外科速報. 1. 73 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2018-02-02  

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