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Local Factors in Bone Metabolism of Experimental Osteoporosis

Research Project

Project/Area Number 02670654
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Orthopaedic surgery
Research InstitutionEhime University

Principal Investigator

OKUMURA Hideo  Ehime University School of Medicine Associate Professor, 医学部, 助教授 (60115813)

Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1992: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1991: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1990: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsOsteoporosis / Rat / Northern blot analysis / m-RNA / Local factor / Osteocalcin / 骨芽細胞 / 活性型ビタミンD_3 / 実験的骨粗鬆症 / ノ-ザンブロッティング法 / Cーfos / cーDNA / mーRNA / TGFーβ / CーDNA
Research Abstract

Human osteoporosis is induced by aging, menopause and immobilization. Various hormones which regulate calcium metabolism in the whole body and various local factors which regulate bone metabolism in local bony tissue affect on the pathophysiology of osteoporosis. The expression of mRNA of local factors in the bone cells of osteoporotic tissue induced by immobilization combined with ovariectomy was studied. The mRNA was analyzed by Northern blot method using cDNA probes. The osteocalcin mRNA level in the osteoporotic tibiae significantly increased. The TGF-beta mRNA level increased in bone marrow cells and slightly increased in bone cells in the osteoporotic tibiae. The experiment of administration of dexamethasone was also done. The osteocalcin mRNA level in the tibiae of the rats treated with dexamethasone significantly decreased. Suppressive effect of dexamethasone on osteocalcin expression was persistent for seven days at higher dose. Northern blot analysis showed that both 1,25(OH)_2D_3 and 2beta-(3-hydroxypropoxy)-1,25(OH)_2D_3 caused an increase in osteocalcin mRNA levels in bone. And they increased osteocalcin mRNA levels in osteoblast-like cells ( ROS17/2.8 ) in vitro. In conclusion, general factors ( estrogen, 1,25(OH)_2D_3 ) have an effect on the trascriptional regulation of local factors ( osteocalcin, TGF- beta ) in bone.

Report

(4 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] H.Okumura et al.: "26,27-Hexafluoro-1,25-dihydroxy vitamin D_3(F_6-1,25(OH)_2D_3)Prevents osteoporosis induced by immobiltzation combined with ovariectomy in the rat" Bone and Mineral. 9. 101-109 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Yamamuro et al.: "The effect of alfacalcidol on bone loss in rheumatoid arthritic patients." Int.J.Immunotherapy. 6. 37-46 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] H.Okumura et al.: "Immobilization combined with ovariectomy and the effect of active vitamin D_3 analogues in the rat." Cells and Materials. Suppl.1. 125-130 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] S.Yoshida et al.: "Microstructural changes of osteopenic trabeculae in the rat." Bone. 12. 185-194 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] S.Ohra et al.: "Fracture healing induces expression of the Proto-oncogene C-fos in vivo." Federation of European Bio chemical Societies(FEBS). 284. 42-45 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Ikeda et al.: "The effect of active vitamin D_3 analogues and dexamethasone on expression of osteocalcin gene in rat tibiae in vivo." Biochem.Biophys.Res.Commun.163. 1231-1235 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 奥村 秀雄(分担執筆)編集山本 吉藏: "図説整形外科診断治療講座8骨粗鬆症" メジカルビュー社, 213(112-119) (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] H.Okomura, et al.: "26,27-Hexafluoro-1,25-dihy-doxyvitamin D_3 (F_6-1,25(OH)_2D_3) prevents os-teoporosis induced by immobilization combined with ovariectomy in the rat." Bone and Mineral. 9. 101-109 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Yamamuro, et al.: "The effect of alfacalcidol on bone loss in rheumatoid arthritic patients." Int. J. Immunotherapy. 6. 37-46 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] H.Okumura, et al.: "Immobilization combined with ovariectomy and the effect of active vitamin D_3 analogues in the rat." Cells and Materials. Suppl.1. 125-130 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] S.Yoshida, et al.: "Microstructural changes of osteopenic trabeculae in the rat." Bone. 12. 185-194 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] S.Ohta, et al.: "Fracture healing induces expression of the proto-oncogene c-fos in vivo." Federation of European Bio-chemical Societies (FEBS). 284. 42-45 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Ikeda, et al.: "The effect of active vitamin D_3 analogues and dexamethasone on expression of osteocalcin gene in rat tibiae in vivo." Biochem. Biophys. Res. Commun.163. 1231-1235 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] H.Okumura: "Experimental Osteoporosis" Illustrated Orthopaedic Diagnosis and Treatment Lecture 8 edit. by K.Yamamoto Medical View, Tokyo ( in Japanese ). 112-119 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Ikeda et al.: "The effect of active vitamin D_3 and logues and dexamethasohe on expression of osteocalcin gene in rat tibiae in vivo." Biochem. Biophys. Res. Commun.163. 1231-1235 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] K. Inoue et al.: "Metabolic bone disease following gasfrectomy: assessment by dual energy x-ray absorptibmetry." Br. J. Surg.79. 321-324 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 奥村 秀雄: "動物実験から見た骨粗鬆症の病態" 日本老年医学会雑誌. 29. 253-256 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 山田 洋司 他: "DEXA (Dual Energy X-ray Absorptiometry)を用いた腰椎骨塩量の検討" 愛媛医学. 11. 114-117 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] H.Okumura et al.: "Immobilization comfined with ovariectomy,and the effecc of active vitamin D_3 analogues in the rat." Cells and Materials. Suppl.1. 125-130 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] S.Yashida et al.: "Microstructural changes of osteopenic trabeculae in the rat" Bone. 12. 185-194 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] S.Ohta et al.: "Fracture healing induces expression of the protoーoncogene cーfos in vivo" Federation of European Biochemical Societies(FEBS). 284. 42-45 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] H.Okumura et al.: "26,27ーHexafluoroー1,25ーdihydroxyvitamtn D_3(F_6ー1,25(OH)_2D_3)prevents osteoporosis induced by immobilization combined with ovariectomy in the rat" Bone and Mineral. 9. 101-109 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 西井 易穂,小林 正,奥村 秀雄: "2βー(3ーhydroxy propoxy)ー1α,25 dihydroxyvitamin D_3の作用と骨粗鬆症治療剤としての可能性" 骨粗鬆症研究の進歩. 5. 115-119 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] T.Yamanuro et al.: "The effect of alfacalcidol on bone loss in rheumatoid arthritic patients" Int,J.Immunotherapy. VI. 37-46 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 奥村 秀雄(分担執筆)編集 山本 吉藏: "図説 整形外科診断治療講座8 骨粗鬆症 分担(112ー119)" メジカルビュ-社, 213 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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