| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1991: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1990: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Research Abstract |
l. Clinical observations of plasma fibronectin (pFN). Using immunoturbidimetric assay, we measured PFN levels of 24 healthy controls and 61 patients with urological carcinomas. (1) pFN levels before treatment : The mean pFN level was 379, mug/ml and 356mu g/ml in healthy controls and patients, respectively, and there was no significant difference. Also no difference was found between patients with metastasis and without metastasis. The pFN level in patients with poor prognosis(300mu g/ml)was significantly lower than that in patients with good prognosis (410mu g/ml). (2) pFN levels duriirg conservative treatments : The pFN level in patients with a rapid progressive disease (287mu g/ml)was significantly lower than in those with a slow progressive disease (327, u g/ml). These data suggest that the low pFN level predicts a rapid progression of disease and poor prognosis. (3) Effect of therapy on the pFN level : ln 3 of 7 patients, the pFN levels decreased (50- 180mu g/ml) during chemothera
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py. Hyperthermia, radiation and IFN therapy did not affect pFN levels. 2. pFN levels in nude mice with subcutaneously inplanted tumors of transitional cell carcinoma, renal cell carcinoma and breast cancer cells : (1) There was no difference between these cell lines. (2) The pFN level of controls was 712mu g/ml and t hos e in 2, 4, 9 and 14 weeks after tumor inplantation were 652, 844, 902 and 730 mug/ml, respectively. There was no significant difference. Metastasis and chachectic state did not affect the pFN level. In mice, because FN activity varied according to the FN measuring kit, pFN levels differed considerably. As the volume of murine plasma in the samples was small, we could not measure them repeatedly. Also some samples were suspected to be serum rather than plasma. Therefore other parameters are needed to improve the means of measuring and to estimate them comprehensively. 3. Results of hyperthermia : The survival fraction of cultured bladder carcinoma cells was about 10^<-1> at 43ﾟC. We tried to heat up subcutaneously inplanted tumors in nude mice by using a THERMOTRON- RF- I. V. But it was impossible to evaluate the effect of local hyperthermia on nude mice, because almost all of them died after several experiments. Less
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