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Effects of Growth Factors on Hormone Release and Intracellular Ca^<2+> of granulosa cells

Research Project

Project/Area Number 02670735
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionTokyo Medical and Dental University

Principal Investigator

KAMADA Shusaku  Tokyo Medical and Dental Univ. Research associate, 医学部, 助手 (80169606)

Co-Investigator(Kenkyū-buntansha) KUBOTA Toshiro  Tokyo Medical and Dental Univ. Lecturer, 医学部, 講師 (50126223)
ASO Takeshi  Tokyo Medical and Dental Univ. Professor, 医学部, 教授 (60093176)
Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1992: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1991: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1990: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsIGF-II / IGF-I / granulosa cell / Steroid hormone / Cell proliferation / cyclic AMP / intracellular Ca^<2+> / Autocrine control / C-AMP / IGFーII / IGFーI / steroidogenesis / cーAMP / autocrine調節 / ETー1 / 細胞内カルシウム濃度 / イノシト-ルフォスフェイト / ホルモン分泌
Research Abstract

The effects of insulin-like growth factor-II (IGF-II) on the proliferation and differentiation of ovarian granulosa cells were studied in vitro. IGF-II significantly increased basal progesterone secretion in granulosa cells at concentrations of 1 - 100 ng/ml. A stimulatory effect was also observed in gonadotropin-stimulated porcine granulosa cells treated with IGF-II. The secretion of estradiol by basal and gonadotropin-stimulated porcine granulosa cells was also significantly increased by adding IGF-II. IGF-II led to dose-dependent increases in [^3H]thymidine incorporation into DNA. Treatment with IGF-II produced a significant increase in the basal accumulation of cyclic AMP in porcine granulosa cells. However, no change of intracellular Ca^<2+> concentration by IGF-II was noted. IGF-II produced effects in accumulation that were similar to those of IGF-I. Our findings suggest that IGF-II may be a general stimulation in the proliferation and differentiation of granulosa cells, and that cyclic AMP may be a second messenger for these effects of IGF-II.
Moreover, IGF-II concentrations were measured using an RIA technique in human follicular fluid (FF) obtained from 20 patients who were undergoing IVF and ET. The large quantity of IGF-II in FF was observed and significant positive correlations were observed between IGFs (IGF-I and IGF-II) and sex steroid hormones. These findings suggest IGFs in FF may play possibly some role in ovarian steroidogenesis.

Report

(4 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (33 results)

All Other

All Publications (33 results)

  • [Publications] Shusaku KAMADA: "Effects of Insulin-Like Growth Factor-II on Prolife-ration and Differentiation of Ovarian Granulosa Cells" Hormone Research. 37. 141-149 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shusaku KAMADA: "Direct effects of bromocriptine on the Steroidogenic capability of Porcine granulisa cells" Acta Endocrinologica. 126. 338-344 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Toshiro KUBOTA: "Insulin-like growth factorll in follicular fluid of the patients with in vitro fertilization and embryo transfer" Fertility and Sterility in press. in press. (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 久保田 俊郎: "Insulin-like growth factor-I及びIGF-IIのヒト脱落膜・羊水系Prolactin分泌に及ぼす影響" 日本産科婦人科学会雑誌. 43. 1515-1520 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 久保田 俊郎: "ヒト卵胞液中のInsulin-like growth factor-II及びIGF-Iレベルの検討" 日本受精着床学会雑誌. 9. 63-65 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 久保田 俊郎: "羊水中におけるProlactinとInsulin-like Growth Factor-Iとの関連性に関する研究" 日本受精着床学会雑誌. 8. 276-278 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Toshiro KUBOTA(分担): "Placenta:Basic Research for clinical" KARGER, 213 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kamada,S., Kubota,T., Taguchi,T., Wen-Rong,H.Sakamoto,S., Aso,T.: "Effects of insulin-like growth factor-II on proliferation and differentiation of ovarian granulosa cells." Hormone Research. 37. 141-149 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kamada,S., Kubota,T., Taguchi,M., Aso,T.: "Direct effects of bromocriptine on the steroidogenic capability of porcine granulosa cells." Acta Endocrinologica(Copenhagen). 126. 338-344 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kubota,T., Kamada,S., Ohara,M., Taguchi,M., Sakamoto,S., Shimizu,Y., Aso,T.: "Insulin-like growth factor II in follicular fluid of the patients with in vitro fertilization and embryo transfer." Fertility and Sterility. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kubota,T., Kamada,S., Taguchi,M., Aso,T.: "Determination of insulin-like growth factor-II in feto-maternal circulation during human pregnancy." Acta Endocrinologica(Copenhagen). 127(4). 359-365 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kubota,T., Kamada,S., Ohara,M., Koi,H., Sen,K., Aso,T.: "Studies on insulin-like growth factor-II and IGF-I in human follicular fluids." Journal of Fertilization and Implantation. 8(1). 63-65 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kubota,T., Kamada,S., Taguchi,M., Aso,T.: "The effects of insul in-like growth factor-I(IGF-I) and IGF-II on prolactin release from human decidua and amniotic fluid circulation." Acta Obstetrica et Gynaecologica Japonica. 43(11). 1515-1520 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kubota,T., Kamada,S., Taguchi,M., Nishi,N., Aso,T.: "The correlation between prolactin and insulin-like growth factor-I levels in human amniotic fluid." Journal of Fertilization and Implantation. 8. 276-278 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kubota,T. and Aso,T.: "Influence of insulin-like growth factors on human fetoplacental function. In Placenta:" Basic research for clinical application. Soma,H. edt., Basel, Karger,. 115-125 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kubota,T. Kamada,S., Taguchi M. and Aso,T.: "The relationship between insulin like growth factors and prolactin in human decidual-amniotic compartment." Kyoto prolactin conference monograph, Edt. Hoshino K. Shinko Printing Co. Kyoto. Vol.6.21-30 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Shusaku Kamada: "Effects of Insulin-Like Growth Factor-II on Proliferation and Differentiation of Ovarian Granulosa Cells" Hormone Research. 37. 141-149 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Shusaku Kamada: "Direct effects of bromocriptine on the steroidogenic capability of porcine granulosa cells" Acta Endocrinologica. 126. 338-344 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Toshiro Kubota: "Insulin-like growth factorII in follicular fluid of the patients with in vitro fertilization and embryo transfer" Fertility and sterility. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] 久保田 俊郎: "Insulin-like growth factor-1及びIGF-IIのヒト脱落膜・羊水系prolactin分泌に及ぼす影響" 日本産科婦人科学会雑誌. 43. 1515-1520 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] 久保田 俊郎: "ヒト卵胞液中のInsulin-like growth factor-2及びIGF-Iレベルの検討" 日本受精着床学会雑誌. 9. 63-65 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 久保田 俊郎: "羊水中におけるprolactinとInsulin-like Growth Factor-Iとの関連性に関する研究" 日本受精着床学会雑誌. 8. 276-278 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Toshiro Kubota(分担): "Placenta:Basic Research for Clinical Application" KARGER, 213 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] 久保田 俊郎: "Insulinーlike growth factorーI及びIGFーIIのヒト脱落膜・羊水系prolactin分泌に及ぼす影響。" 日本産科婦人科学会雑誌. 43. 1515-1520 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 久保田 俊郎: "ヒト卵胞液中のInsulinーlike growth factorーII及びIGFーIレベルの検討" 日本受精着床学会雑誌. 9. 63-65 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Toshiro Kubota: "Role of argiotensin on paracrine prolactin release in the pituitary glard and its possible effects on orarian function" Hormone Research. 35. 13-20 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Shnsaku Kamada: "Direct effects of bromocriptine on the steroidogenic capability of porcine granulosa cells" Acta Endocrinologica. 126. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Shusaku Kamada: "Direct effect of endothelinー1 on the granulosa cells of the porcine ovary" Journal of Endocrinology. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Shusaku Kamada: "Effects of insulinーlike growth factorーII on proli Peration and differeritiation of ovarian grannlosa cells" Biology of Repraduction.

    • Related Report
      1991 Annual Research Report
  • [Publications] Shusaku Kamada: "Direct effects of bromocriptine on the steroidogen capability of porcine grannlosa cells" Fertility and Sterility.

    • Related Report
      1990 Annual Research Report
  • [Publications] Shusaku Kamada: "Endothelinー1 stimulates Ca^<2+> mobilization and inositol phosphnte generation in porcine cultured granulosacolls" FEBS Lett.

    • Related Report
      1990 Annual Research Report
  • [Publications] 久保田 俊郎: "羊水中におけるprolactinとInsulinーlike growth factorーIとの関連性に関する研究" 日本受精着床学会雑誌.

    • Related Report
      1990 Annual Research Report
  • [Publications] 久保田 俊郎: "Insulinーlike growth factorーI及びIGFーIIのヒト脱落腰・羊水系prolaotin分泌に及ぼす影響" 日本産科婦人科学会雑誌.

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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