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Virologic study and cyokine treatment of cervical adenocarcinoma.

Research Project

Project/Area Number 02670748
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionSaga Medical School

Principal Investigator

IWASAKA Tsuyosi  Saga Medical School Assistant Professor, 医学部, 助教授 (60117067)

Co-Investigator(Kenkyū-buntansha) 林 嘉信  佐賀医科大学, 医学部, 助手 (90156464)
Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1992: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1991: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1990: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsCervical adenocarcinoma / Human papillomavirus / P53 gene / Interferon-r / Etoposide / Mitomycin C / Chemotherapy / 子宮頸部腺癌 / VP16 / ヒトパピロ-マウイルスDNA / インタ-フェロンγ / ヒト乳頭腫ウイルス(HPV) / インタ-フェロンγ(IFNーγ) / 腫瘍壊死因子(TNF) / 抗癌剤
Research Abstract

(1)To establish the effective chemotherapy regimen for patients with cervical adenocarcinoma, 15 conventional antitumordrugs were tested for their anticellular effects on 5 cervical adenocarcinoma cell lines. In addition to interferon-, etoposide(VP-16) and mitomycin C(MMC) were considered to be the best drugs for combination chemotherapy for cervical adenocarcinoma cell lines.
(2) Among the five adenocarcinoma cell lines, HPV 18 DNA was detected in four cell lines, however, none of the HPV DNA was detected in one cell line(OMC-4). Furthermore, a point mutation of p53 gene was demonstrated in this cell line, while no abnormality was observed in the remaining 4 cell lines.
(3) Thirty-two cervical adenocarcinomas and adenosequamous carcinomas were examined to search for human papillomaviruses using polymerase chain reaction system. HPV 16 and HPV 18 DNAs were detected in 22% and 16% of these carcinomas, respectively.
(4) Experiments using nude mice bearing transplanted cervical adenocarcinoma cell lines revealed that interferon-, VP-16, and MMC were effective against tumors in vivo both by single agent and by combination of these.
(5) Clinical trial by combination of 3 antitumor agents, CDDP, VP-16, and MMC, is now on going for the treatment of patients with cervical adenocarcinoma.

Report

(4 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] 原 浩一,岩坂 剛,杉森 甫: "子宮頸部腺癌" Oncelogy & Chemotherapy. 8. 19-24 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 原 浩一,岩坂 剛 杉森 甫: "婦人科領域におけるサイトカインの臨床応用" Oncopogy & Chemotherapy. 8. 252-255 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Iwasaka,K.Hara,Y.Hayashi,M.Yokoyama,et al.: "Antitumor effects of human recombinant interferon-and tumor necrosis factor on five cervical adenocar-cinoma cell lines,in vivo and in vitro." Gynecologic Oncology. 42. 39-43 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] N.Matsyo,T.Iwasaka,Y.Hayashi,K.Hara,et al.: "A polymerase chain reaction analysis of human papilloma-Virus in adenocarcinoma and adenosquamous carcinoma of the uterine cervix." Int.J.Gynecol.Obstet.(1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Iwasaka,M.Oh-uchida,N.Matsuo,M.Yokoyama,et al.: "Correlation between HPV positivity and state of the p53 gene in cervical carcinoma cell lines." Gynecologic Oncology. 48. 104-108 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 原 浩一,岩坂 剛,杉森 甫: "“子宮頸部腺癌の化学療法"子宮頸部腺癌における各種抗癌剤サイトカインの併用療法に関する研究" Oncology & Chemitherapy. 9. (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T. Iwasaka, K. Hara, Y. Hayashi, M. Yokoyama, et al: "Antitumor effects of human recombinant interferon and tumor necrosis factor on five cervical adenocarcinoma cell lines." Gynecologic Oncology. 42. 39-43 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] N. Matsuo, T. Twasaka, Y.Hayashi, K. Hara, et al: "A polymerase chain reaction analysis of human papilloma virus in adenocarcinoma and adenosequamous carcinoma of the uterine cervix." Int. J. Gynecol. Obstet. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T. Iwasaka, M. Oh-uchida, N. Matsuo, Y.Yokoyama, et al: "Correlation between HPV positivity and state of the p53 gene in cervical carcinoma cell lines." Gynecologic Oncology. 48. 104-108 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 原 浩一,岩坂 剛,杉浦 甫: "子宮頸部腺癌" Oncology & Chemotherapy. 8. 19-24 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 原 浩一,岩坂 剛,杉浦 甫: "婦人科領域におけるサイトカインの臨床応用" Oncology & Chemotherapy. 8. 252-255 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Iwasaka,K.Hara,Y.Hayashi,M.Yokoyama,et al.: "Antitumor effects of human recombinant interferon and tumor necrosis factor on five cervical adenocarcinoma celllines,in vivo and in vitro." Gynecologic Oncology. 42. 39-43 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] N.Matsuo,T.Iwasaka,Y.Hayashi,K.Hara,et al.: "A polymerase chain reaction analysis ofhuman papillomavirus in adenocarcinoma and adenosquamous carcinoma of the uterine cervix." Int.J.Gynecol.Obstet.(1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Iwasaka,M.oh-uchida,N.Matsuo,M.Yokoyama,et al.: "Correlation between HPV positivity and state of the p53 gene in cervical carcinoma cell lines." Gynecologic Oncology. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Iwasaka,K.Hara,Y.Hayashi,M.Yokoyama,et al.: "Antitumor effects of human recombinant interferon‐and tumor necrosis factor on five cervical adenocarcinoma cell lines,in vivo and in vitro." Gynecologic Oncology. 42. 39-43 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Iwasaka,K.Hara,Y.Hayashi,et al.: "Antitumor effects of human recombinant interferonーγ and tumor necrosis factor on five cervical adenocarcinoma cell lines,in vivo and in vitro." American Journal of Obstetrics and gynecology.

    • Related Report
      1990 Annual Research Report
  • [Publications] Y.Hayashi,T.Iwasaka,M.Ohーuchida,et al.: "Human papillomavirus in adenocarcinoma and adenosquamous carcinoma of the uterine cervix:A polymerase chain reaction analysis." American Journal of Obstetrics and gynecology.

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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