| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1991: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1990: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Research Abstract |
1 The glass column-continuous density gradient centrifugation, established in the present study, was capable of separating progressively motile sperm from raw human semen. The preparation was free from seminal plasma components, and was suitable for quantitative measurement of sperm motility by using the computer assisted sperm motility analyzer.
2 Effect bioligically active peptides, bradykinin, angiotensin II and enkephalin, on highly purified human sperm motility were estimated by using the computer assisted sperm motility analyzer. They stimulated sperm motility, especially for sperm-progressive velocity in the absence of seminal plasma. The effects were, however, disappeared by addition bf seminal plasma. Proteinases in seminal plasma might inactive them.
3 Pentoxifilline(PXF), a phosphodiesterase inhibitor, stimulate sperm motility in the presence of seminal plasma. In the case of retro-grade ejaculation, semen was refluxed in blader, and even replaced urine to culture medium, contamination of urine decreased sperm viability. The culture medium with PXF intorduced in blader before ejaculation protected sperm viability. The recovered sperm without loss of motility was provided for IVF-ET, resulting one successful pregnancy.
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