| Project/Area Number |
02670797
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Ophthalmology
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| Research Institution | Nihon University |
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Principal Investigator |
SAKIMOTO Takashi Nihon Univ. School of Med. Associate Prof., 医学部, 助教授 (70111515)
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| Co-Investigator(Kenkyū-buntansha) |
EBATO Bunshu Nihon Univ. School of Med. Resident, 医学部, 助手 (00158784)
KASAI Hiroshi Nihon Univ. School of Med. Lecturer, 医学部, 講師 (80133437)
周藤 昌行 日本大学, 医学部, 助手
冨田 隆之 日本大学, 医学部, 助手 (70172187)
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| Project Period (FY) |
1990 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1991: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1990: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Conneal stroma / Corneal epithelium / reciprocal relation / neovascularization / infiltrated cells / conjunctival flap / Limbal transplant |
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| Research Abstract |
To investigate the effects of conjunctival flap to the damaged cornea, such as bullous keratopathy and vascularized cornea, epithelial denudation of whole cornea including limbal area was first in place done with n-heptanol. After the conjunctival flapping, early changes which were in common with all damaged cornea first appeared in the anterior stroma of the cornea. Superficial edema was temporally continued by the stage into the infiltration of fibroblastic cells. A convalescent stage with normal keratocytes finally followed the absorption of edema with capillaries in the conjunctival flap. Comparing the pathological stroma of the cornea conjunctival flap was hardly attached to the surface of normal stroma.
Basal cells of limbal epithelium are considered as STEM CELL of corneal epithelium. To clarify the interaction between corneal and conjunctival epithelial cells, superficial limbal grafts were transplanted reversely to the denuded limbus. As a result, regenerated epithelial cells derived from limbal conjunctiva were most active in anyother part of the epithelium.
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