Study on muscle fiber composition and biochemical properties of the adductor mandibulae in fishes showing different feeding activities
| Project/Area Number |
02670813
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphological basic dentistry
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
MAEDA Norihiko Hiroshima University School of Dentistry, Oral Anatomy, Professor, 歯学部, 教授 (60049418)
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| Co-Investigator(Kenkyū-buntansha) |
HOSOI Mitsuteru Hiroshima University School of Dentistry, Associated Professor, 歯学部, 助教授 (00034190)
NISHIMORI Toshikazu Miyazaki Medical College, Biology, Professor, 医学部, 教授 (20112211)
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| Project Period (FY) |
1990 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1991: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1990: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | Fish / Adductor mandibulae / Muscle fiber composition / succinic dehydrogenase / LDH isozymes / フナ / ウグイ / オイカワ / 閉顎筋 / 筋線維構成 / LDHアイソザイム / Oncorhynchus masou / 開顎筋 / Lactate dehydrogenase(LDH)活性 / アイソザイムパタ-ン |
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| Research Abstract |
Lactate dehydrogenase (LDH) isozyme distributions in the jaw-closing and -opening muscles, myotome muscle, heart, and eye of the parr-type and smolt-type fish in a primitive teleostean, Oncorhynchus masou (O.masou),which have adapted to the rapid current at the upper reach of a river and in the mild current at the middle reach,respectively, were investigated by horizontal agarose gel electrophoresis. Both jaw muscles of the parr-type fish had following three isozymes: the slowest anodal migrative LDHA_4, the fastest anodal migrative LDHB_4, and an isozyme migrating between them. The major isozyme was LDHA_4, amounting over 80% of total LDH activity. The white portion of the myotome muscle which is the dominant part and contracts fast and forcefully, and the heart muscle which contracts slowly and continuously had only one isozyme LDHA_4 or LDHB_4, respectively. The red portion of the myotome muscle had three isozymes, though LDHB_4 one was predominant. These facts suggest that jaw-clos
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ing and-opening muscles and myotome muscle are well adapted for swift feeding behavior in the rapid current, because forceful and rapid contractions of those muscles would be needed to catch the insects floating down in the rapid current. With growth of length of the fish, LDHB_4 isozyme activity significantly increased in the jaw-closing muscle, but not in the jaw-opening muscle. Furthermore, the smolt-type fish which lives in open-habitats of rivers and lakes and feed mainly on other species of fish, had a higher level of LDHB_4 isozyme in the jaw-closing muscle than the parr-type fish.
Thus, the higher levels of LDHB_4 isozyme in the jaw-closing muscles of the larger parr-type fish and the smolt-type fish, and appearance of LDHB_4 isozyme in the white portion of the myotome muscle of the smolt-type fish, suggest that the function of the muscles may change with alternation of food habits and locomotion manners respectively, in this species. Moreover, these data may be very important for explanation of the physiological meaning of each type of isozyme of LDH in the muscles. Less
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Report
(4 results)
Research Products
(2 results)
