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Induction of cytokines by oral bacterial stimulation and its arthropathic properties

Research Project

Project/Area Number 02670824
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionTohoku University

Principal Investigator

RIKIISHI Hidemi  Tohoku Univ. Dentistry Assistant Prof., 歯学部, 助手 (70091767)

Co-Investigator(Kenkyū-buntansha) KUMAGAI Katsuo  Tohoku Univ. Dentistry, 歯学部, 教授 (00005018)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1991: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1990: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsPeptidoglycan / Cytoplasmic membrane protein / GM-CSF / Arthropathic reaction / ペプチドグリカン刺激 / サイトカイン / マウス系統差 / 急性関節炎
Research Abstract

The activities of peptidoglycan(PG)fragments derived from gram-positive cocci were examined to proliferate spleen mononuclear cells(SMNC)from murine various strains and to produce cytokines in vitro, and also examined to induce the inflammatory reaction in vivo. The proliferation of SMNC from C3H/HeN mice to PG in vitro were maximum on day 3, and greater than that of SMNC from Balb/c mice. It was indicated that the responsive cells of SMNC from C3H/HeN mouse to PG were mainly Ia^+ Mac^<2+> cells. mRNA transcripts of GM-CSF were detected in the lungs of C3H/HeN mouse much stronger than that of Balb/c mouse after 3 hr of injection. Balb/c mice were susceptible to acute arthritis after PG injection, whereas C3H/HeN and C57BL/6 mice failed to produce acute arthritis. The cytoplasmic membrane protein(MAP)of Streptococcus pyogenes, but not PG and others, when incubated with human peripheral blood lymphocytes, induced polyclonal activation of T cells after 5-7 days of incubation without IL-2 production. The activated T cells included CD^<4+> CD^<8-> helper T cells. We have obtained some informations on the responsibility of mononuclear cells to bacterial cell components, especially cytokine production and activated cells. These factors form a complex net work, and a complete understanding of the mechanisms involved in the arthritis to bacterial subfraction, must await detailed studies.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] 安保 徹: "Induction of human TCRγ δ^+ and TCR γ δ^ーCD2^+CD3^ー double negative lymphocytes by bacterial stimulation." International Immunology. 2. 775-785 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 関 修司: "Identification of activated T cell receptor γ δ lymphocytes in the liver of tumorーbearing hosts." Journal of Clinical Immunology. 86. 409-415 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 川村 勝: "Further characterization of the autologous mixedーlymphocyte reaction;Induction of double negative γ δ lymphocytes." Cellular Immunology. 133. 468-483 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 関 修司: "An appropriate in vitro culture condition for the induction of human TCR γ δ^+ cells by heatーkilled bacteria." Journal of Immunological Methods. 139. 31-40 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 安保 徹: "The appearance of T cells bearing selfーreactive T cell receptor in livers of mice injected with bacteria." Journal of Experimental Medicine. 174. 417-424 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 佐藤 俊裕: "Polyclonal activation of human lymphocytes and induction of cytotoxic lymphocytes by streptococcal preparations." Biotherapy. 4. 53-63 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 熊谷 勝男: "Periodontal Disease:Pathogens & Host Immune Responses" Quintessence Publishing Co.Ltd., 410 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Abo: "induction of human TCR_<gamma>delta^+ and TCR_<gamma>delta^-CD2^+CD3^- double negative lymphocytes by bacterial stimulation." International Immunology. 2. 775-785 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S. Seki: "Identification of activated T cell receptor gamma^<delta> lymphocytes in the liver of tumor-bearing hosts." Journal of Clinical Immunology. 86. 409-415 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M. Kawamura: "Further characterization of the autologous mixed-lymphocyte reaction : Induction of double negative gamma^<delta> lymphocyes." Cellular Immunology. 133. 468-483 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S. Seki: "An appropriate in vitro culture condition for the induction of human TCR _<gamma>delta^+ dells by heat-killed bacteria." Journal of Immunological Methods. 139. 31-40 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Abo: "The appearance of T cells bearing self-reactive T cell receptor in livers of mice injected with bacteria." Journal of Experimental Medicine. 174. 417-424 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Sato: "Polyclonal activation of human lymphocytes and induction of cytotoxic lymphocytes by streptococcal preparations." Biotherapy. 4. 53-63 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K. Kumagai: "Induction of cytokine production and lymphocyte proliferation due to stimulation of bacterial components." Periodontal Diseases : pathogens & Host Immune Responses. 329-340 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 安保 徹: "Induction of human TCR γδ^+ and TCR γδ^-CD2^+CD3^- double negative lymphocytes by bacterial stimulation." Intenational Immunology. 2. 775-785 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 関 修司: "Identification of activated T cell receptor γδ lymphocytes in the liver of tumorーbearing hosts." Journal of Clinical Immunology. 86. 409-415 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 川村 勝: "Further characterization of the autologous mixedーlymphocyte reaction:Induction of double negative γδ lymphocytes." Cellular Immunology. 133. 468-483 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 関 修司: "An appropriate in vitro culture condition for the induction of human TCR γδ^+ cells by heatーkilled bacteria." Journal of Immunological Methods. 139. 31-40 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 安保 徹: "The appearance of T cells bearing selfーreactive T cell receptor in livers of mice injected with bacteria." Journal of Experimental Medicine. 174. 417-424 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 佐藤 俊裕: "Polyclonal activation of human lymphocytes and induction of cytotoxic lymphocytes by streptococcal preparations." Biotherapy. 4. 53-63 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 熊谷 勝男: "Periodontal Disease:Pathogens & Host Immune Responses" Quintessence Publishing Co.Ltd., 410 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 熊谷 勝男: "RAの素因を考えるー動物モデルからの考察ー" 第3回RA早期診断研究会. 3. 64-82 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Katsuo Kumagai: "PERIODONTAL DISEASE:PATHOGENS and HOST IMMUNE RESPONSES" S.Hamada,S.C.Holt and J.R.McGhee, (1991)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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