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Cytotoxic Effects of Periodontopathic Bacteria on Human Gingival Epithelial Cell Cultured in Serum-Free Medium.

Research Project

Project/Area Number 02670849
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Conservative dentistry
Research InstitutionTokyo Medical and Dental University

Principal Investigator

IZUMI Yuichi  Tokyo Medical and Dental University, Department of Periodontology, Instructor, 歯学部, 助手 (60159803)

Co-Investigator(Kenkyū-buntansha) NOGUCHI Kazuyuki  Tokyo Medical and Dental University, Department of Periodontology, Instructor, 歯学部, 助手 (90218298)
UMEDA Makoto  Tokyo Medical and Dental University, Department of Periodontology, Instructor, 歯学部, 助手 (90193937)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1991: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1990: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsCulture in Serum Free Medium / Gingival Keratinocyte / Periodontal Pathogens / Cell Proliferation / Protein Synthesis / Polymorphonuclear Leukocyte / Lysosomal Enzyme / Cell Detachment / 歯肉上皮細胞 / 生物学的活性
Research Abstract

The purpose of this investigation was to study the effects of selected periodontal pathogens on cell proliferation, protein synthesis and cell injury in vitro.
The ultrasonic extracts from microorganisms included A. actinomycetemcomitans, P. gingivalis, P. intermedia, C. sputigena, E. corrodens, F. nucleatum, A. viscosus and St. mutans. Target cells were keratinocytes SCC9 and gingival keratinocytes cultured in serum-free medium. The results showed that cell proliferation and rate of protein synthesis by SCC9 were strongly inhibited by E. corrodens ; at similar concentrations A. actinomycetemcomitans, P. gingivalis and C. sputigena also significantly reduced the proliferation and synthesis whereas P. intez-media, F. nucleatum, A. viscosus and St. mutans had only minor ef f ects. These results indicate that keratinocyte function may be modulated by periodontopathic bacteria.
The cell detachment assay was subjected by polymorphonuclear leukocytes and/or bacterial ultrasonic extracts from A … More . actinomycetemcomitans, B. forsythus, F. nucleatum, C. sputigena and E. corrodens. The bacterial extracts alone caused no cell disruption of the keratinocyte monolayers. Polymorphonuclear leukocyte alone caused only a minimal cell detachment. On the contrary, when the bacterial extracts were added to the co-culture of keratinocytes and polymorphonuclear leukocytes, cell detachment of keratinocytes was observed except for the bacterial extract f rom E. corrodens. The effect of A. actinomycetemcomitans was strongest. This effect was heat labile and not inhibited by polymyxin B. Cell detachment was inhibited by alpha 1-antitrypsin but not by catalase and superoxide dismutase. Hydrogen peroxide and leukocyte elastase also caused keratinocyte detachment, and keratinocyte lysis was not observed. These results indicate that polymorphonuclear leukocyte can cause non-lytic cell detachment of the gingival keratinocytes when encountering some bacteria, which may lead to the increase of the permeability of the keratinocyte layers. Less

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] 梅田 誠: "PapillonーLefevre症候群患者2名臨床的、免疫学的および細菌学的検査所見および治療経過について。" 口腔病学会雑誌. 57. 430-440 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 和泉 雄一: "サンドイッチELISA法による歯肉溝滲出液中の酵素測定法とその臨床的意義。" 歯界展望. 79. 315-322 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 杉山 栄一: "歯周疾患関連細菌抽出物の多形核白血球を介した口腔角化細胞に対する作用。" 口腔病学会雑誌. 59. 13-25 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kazuyuki NOGUCHI: "Time-dependent Increase of Platelet-activating Factor Acetylhydrolase in the Culture Medium of Mouse Calvaria" Platelets. 3. 23-27 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 和泉 雄一: "培養口腔角化細胞の機能に対する各種歯周病原性細菌の影響" 日本歯科保存学会雑誌. 35. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Makoto UMEDA: "Clinical, Bacteriological and Immunological Examination and Treatment of Two Papillon-Lefevre Syndrome Patients." The Journal of The Stomatological Society. 57-No. 3. 430-440 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yuichi IZUMI: "Sandwich ELISA for Measurement of Elastase, Cathepsin G and Their Inhibitor-Complex Levels in Gingival Crevicular Fluid." Dental Outlook. 72-No. 2. 315-322 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Eiichi SUGIYAMA: "Polymorphonuclear Leukocyte-Mediated Effects on Human Oral Keratinocytes by Periodontopathic Bacterial Extracts." The Journal of The Stomatological Society. 59-No. 1. 13-25 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kazuyuki NOGUCHI: "Time-dependent Increase of Platelet-activating Factor Acetyldrolase in the Culture Medium of Mouse Calvaria." Platelets. 3. 23-27 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yuichi IZUMI: "Cytotoxic Effects of Periodontopathic Bacterial Extracts on Human Oral Keratinocytes in Culture." The Japanese Journal of Conservative Dentistry. 35. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 和泉 雄一: "培養口腔角化細胞の機能に対する各種歯周病原性細菌の影響" 日本歯科保存学会雑誌. 35. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 杉山 栄一: "歯周疾患関連細菌抽出物の多形核白血球を介した口腔角化細胞に対する作用" 口腔病学会雑誌. 59. 13-25 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kazuyuki Noguchi: "Timeーdependent Increase of Plateletーactivating Factor Acetylhydrolase in the Culture Medium of Mouse Calvaria." Platelets. 3. 23-27 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 和泉 雄一: "サンドイッチELISA法による歯肉溝滲出液中の酵素測定法とその臨床的意義" 歯界展望. 79. 315-322 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 和泉 雄一: "培養歯肉上皮細胞の機能に対する各種歯周病原性細菌の影響" 日本歯科保存学雑誌. 第33巻(suppl). 38-38 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 和泉 雄一: "培養歯肉上皮細胞の機能に対する各種歯周病原性細菌の影響" 口腔病学会雑誌. 第57巻. 451-451 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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