• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Effects of Periodontic Restration of Periodontitis caused by Lipopolysaccharide Bacteria

Research Project

Project/Area Number 02670863
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Conservative dentistry
Research InstitutionFukuoka Detal College

Principal Investigator

HARAGA Yoshiaki  Fukuoka Dental College Endodontics and Periodontics Lecturer, 歯学部, 講師 (10105702)

Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1991: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1990: ¥600,000 (Direct Cost: ¥600,000)
KeywordsLipopolysaccharide / Gingival mitochondria / Respiratory control / ミトコンドリア / Bacteroides gingivalis
Research Abstract

Lipopolysaccharide (LPS) was extracted from Bacteroides gingivalis (B. g), which is thought to be one of the bacteria that causes periodontitis, and treated with liver and heart mitochondria. The effects were examined according to the ability of the mitochondria to respire. E. coli LPS was used as the control subject. Gingiva was extracted from a dog and the gingival mitochondria preparation was tested in order to find the effects on gingiva.
The results are as follows :
1. When the liver mitochondria was treated with E. coli LPS at the ratio of 3Omug/mg, the ADP/0 ratio was 32.5% and the respiratory control ratio (RCR) declined by 36.2%. When treated with 40mug/mg of LPS, the mitochondria was completely unable to respire.
2. When the liver mitochondria was treated with B. g LPS at the ratio of 150mug/mg, the ADP/0 ratio was 35.5% and the RCR declined by 39.4%. When treated with 200mug/mg of LPS, the mitochondria was completely unable to respire.
3. When the heart mitochondria was treated with E. coli LPS at the ratio of 25mu/mg, the ADP/0 ratio was 20.0% and the RCR decreased by 20.6%. When treated with 50mug/mg of LPS, respiration was undetectable.
4. When the liver mitochondria was treated with B. g LPS at the ratio of 1, 000mug/mg, the ADP/0 ratio was 30.5% and the RCR was reduced by 44.1%. And resoiration was undetectable when treated with 1.500mug/mg of LPS.
5. When measuring the amount of oxygen depletion in the mitochondria prepared from gingiva, respiratory stroma was observed and an increase in the amount of oxygen depletion was detected when ADP was added ; however, after the ADP was expended, respiratory control was found to be unstable.
Presently, the type, temperature and density of oxygen are being considered in order to prepare gingival mitochondria with respiratory control.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (2 results)

All Other

All Publications (2 results)

  • [Publications] 森 滋康,原賀 義昭,小松 智生,前野 誠,栢 豪洋: "ラット肝,心筋ミトコンドリアの呼吸調節に及ぼすLPSの影響" 日本歯周病学会会誌. 33. 61 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 森 滋康: "ラット肝、心筋ミトコンドリアの呼吸調節能に及ぼすLPSの影響" 日本歯周病学会会誌. 33. (1991)

    • Related Report
      1990 Annual Research Report

URL: 

Published: 1990-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi