| Project/Area Number |
02670898
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
外科・放射線系歯学
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| Research Institution | Yamaguchi University Hospital |
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Principal Investigator |
TSUJI Tatsuo Yamaguchi University Hospital, Dept. of Oral Surgery, Research Associate, 医学部附属病院, 助手 (70144954)
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| Co-Investigator(Kenkyū-buntansha) |
MAEDA Kanji Yamaguchi University Hospital, Dept. of Oral Surgery, Resident, 医学部附属病院, 医員
SASAKI Kohsuke Iwate Medical University School of Medicine, Dept. of Pathology, Professor, 医学部, 教授 (80116722)
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| Project Period (FY) |
1990 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1992: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1991: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1990: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | Fluorescence in situ hybridization / Flow cytometry / Proliferating cell nuclear antigen / c-erbB-2 oncogene / Immunohistochemistry / DNA aneuploidy / AgNORs / Oral cancer / 腫瘍マ-カ- / 腫癌関連抗原 / 増殖細胞核抗原(PCNA) / FISH法 / 染色体異常 / 増殖細胞マ-カ- / フロ-サイトメトリ- / 免疫組織化学 / cーerbBー2遺伝子産物 / KMー93抗体 |
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| Research Abstract |
The numerical aberration of chromosome 17 can be detected by fluorescence in situ hybridization in oral tumors and this correlated with malignant behavior of oral tumors. PCNA immunoreactivity correlated with the differentiation and atypia of the tumor cells in squamous cell carcinoma of the oral cavity. PCNA score was significantly higher in the malignant and pre-malignant lesion, and correlate with the degree of dysplasia in pre-malignant lesions. The mean PCNA score decreased significantly after cancer chemotherapy of squamous cell carcinoma. According to this results, the response of cancer cells to anticancer agents may be estimated by consecutive measurement of PCNA. c-erbB-2 oncoprotein is rarely expressed in malignant salivary gland tumors. However, with a limited number of c-erbB-2 oncoprotein positive cases, it was not possible to correlate the oncogene status with clinical behavior of the tumor. c-erbB-2 did not detected in squamous cell carcinoma, but markedly detected in plemorphic adenoma, and especially in the cells which showed invasive growth activity DNA aneuploidy is not specific for malignant tumors and there was significant difference in DNA index between malignant and benign ledsions. KM-93 antigen detected significantly high level in the patient serum of the salivary gland tumotrs, therefore, it seems that measurement of this antigen is useful method for clinical practice.
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