| Project/Area Number |
02670923
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
小児・社会系歯学
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| Research Institution | The University of Tokushima |
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Principal Investigator |
KAWATA Turushige The University of Tokushima,School of dentistry,Professor, 歯学部, 教授 (40029971)
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| Co-Investigator(Kenkyū-buntansha) |
ZUIKI Yusi The University of Tokushima,School of dentistry,Research associate, 歯学部, 助手 (00211318)
HIURA Kenji University of Tokushima,School of dentistry,Assistant Professor, 歯学部, 講師 (20228696)
SUMITANI Koji The University of Tokushima,School of dentistry,Assistant Professor, 歯学部, 講師 (30206586)
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| Project Period (FY) |
1990 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1992: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1991: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1990: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | osteblast / osteoclast / MC3T3-E1 / orthodontic force / bone resorption / bone formation / osteoclast formation inducing factor / osteoclast formation assay / MC3T3ーE1 / 矯正力負荷装置 / 破骨胞形成促進因子 / MC3T3ーE1細胞 / Prostaglandin E_2 |
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| Research Abstract |
In this study,the relationship between osteoclasts and osteoblasts was focused under the condition of orthodontic force.
The aims and its results were described as follows.
Aim 1.To examine the osteoclast formation modulating factor(s) produced by osteoblastic cells.
Results (1)Conditioned medium including some factors produced by clonal osteoblastic cell line,MC3T3-E1,and primary osteoblastic cells inhibited osteoclast formation. (2)These inhibitory effects were specific in osteoblastic cells. (3)The existence of two humoral factors was indicated.One is osteoclast formation inducing factor with a mol wt of more than 3000 and the other was osteoclast formation inhibitory factor with a mol wt of less than 3000. (4)The inhibitory effect was partially suppressed by indomethacin.And it was reported that MC3T3-E1 produced prostaglandin E_2.Judging from these results,the Inhibitory effects of MC3T3-E1 was due to prostaglandin E_2 in part.
Aim 2.To examine the osteoclast formation modulating factor(s)which produced by osteoblastic cells under the condition of orthodontic force.
Results (1)MC3T3-E1 cells were cultured in petriperm culture dish(Heraeus).When they were cultured under the condition of stretching,osteoclast formation inducing factor was time-dependently produced. (2)It was suggested that osteoclast formation inducing factor supports the proliferation of osteoclast precursor.
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