| Project/Area Number |
02670924
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
小児・社会系歯学
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| Research Institution | Kyushu University |
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Principal Investigator |
IWASE Tatsuo Kyushu University, Department of Preventive Dentistry, Assistant Professor, 歯学部, 講師 (60151734)
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| Co-Investigator(Kenkyū-buntansha) |
SAITO Toshiyuki Kyushu University, Department of Preventive Dentistry, Instructor, 歯学部, 助手 (10170515)
NARA Yoshio Kyushu University, Department of Preventive Dentistry, Associate Professor, 歯学部, 助教授 (90091321)
堀 信顕 九州大学, 歯学部, 講師 (60037520)
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| Project Period (FY) |
1990 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1991: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1990: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | low power laser / He-Ne / electrical stimulation / dental pulp / wound healing / growth factor / rats / 下歯槽神経 / 神経原性炎症 / 刺激伝導 / 色素定量 |
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| Research Abstract |
(A) In previous study, we reported that the membrane potential of pyramidal cell of a brain slice of rat was hyperpolarized and the neuronal membrane was stabilized by He-Ne laser. Therefore, the effect of He-Ne laser on peripheral nerve conduction was studied using an inferior alveolar division of trigeminal nerve in rats. In this experiment, vascular permiability was measured by Pontamin-sky blue which was leaked from the vascular system in the course of antidromic vasodilation. After beginning of of the stimulation only the area supplied by the stimulated nerve turned gradually to a deeper blue. The skin area supplied by the inferior alveolar nerve was indicated by a sharply demarcated area of intense blue dye accumulation. On, the other hand, in the case of the animal exposed to the laser, the demarcated area of intense blue was obviously small, and the intensity of color was less than that of the control.
(B) The role of transforming growth factor-beta (TGF-beta) on He-Ne laser-stimulated wound healing in rats was investigated. Two square skin defects were produced, one on either side of the dorsal midline of each rat. TGF-beta was applied to one defect. The defects to which was TGF-beta was applied healed more rapidly than the control sides. Next, the activity of TGF-beta on the laser-treated sides at day two was significantly higher than that in the non-laser- treated sides. Using an antibody which could neutralize the TGF-beta activity, wound healing in the antibody-applied sides was found to be significantly slower than that in the control sides. Using the antibody after laser irradiation, the result was the same as when the antibody alone was used. From these results, it is suggested that He-Ne lasers stimulate the enhancement of TGF-beta activity on wound healing in rats, and that TGF-beta plays an important role in laser-stimulated woumd healing.
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