| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1991: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1990: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Research Abstract |
As a model for alcohol dehydrogenase-NADH couple, a series of macrocyclic 1, 4-dihydronicotinamides 1a-c linked adenine was prepared via N-[omega-(9-(6-hydroxyhexyl) purin-6-ylamino) alkyl]nicotinamide (a, alkyl = butyl ; b, pentyl ; c, hexyl) by iodination of the hydroxyl group followed by cyclization to the pyridinium salt and reduction. The coordination behavior of 1b, c with magnesium ion in acetonitrile was investigated by ultraviolet and ^1H-NMR spectroscopies, and compared with those of 1-methyl-3-(N-methylcarbamoyl)-1, 4-dihydropyridine (2) and 1-[6-(6-methylamino-9H-purin9-yl) lhexyl-3-(N-methylcarbamoyl)-1, 4-dihydropyridine (3). The adenine moiety in 1b, c accelerates complexation of magnesium ion at the dihydronicotinamide moiety, presumably by acting as a pi electron donor. The reactivity of 1a-c, 2, and 3 toward methyl benzoylformate in the presence of magnesium perchlorate in acetonitrile was kinetically investigated and found to be of the order 1b>1a>3<greater than or equal>1c>2 under the conditions teste
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