| Project/Area Number |
02671027
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | National Institute of Hygienic Sciences |
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Principal Investigator |
TERAO Tadao National Institute of Hygienic Sciences, Division of Drugs, Director, 薬品部, 部長 (60012605)
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| Project Period (FY) |
1990 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1991: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1990: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Growth hormone / Growth hormone receptor / IM-9 cells / Monoclonal antibodies / Ubiquitin / Down-regulation / Phorbol diesters / Phosphorylation / IMー9 / 抗成長ホルモン受容体抗体 / ハイブリド-マ / エビキチン / 抗ユビキチン抗体 |
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| Research Abstract |
Growth hormone-dependent proliferation of IM-9 cells under low-serum conditions was inhibited by the addition of phorbol diesters. The phorbol diesters were found to down-regulate the growthhormone receptor levels. These regulations by phorbol diesters were inhibited by staurosporine or sphingosine, suggesting the involvement of C-kinases. It was also found that phosphorylation of a 55 kDa protein, which was associated with HGHR, was increased.
Monoclonal anti-peptide antibodies against human growth hormone receptor(HGHR)were prepared and characterized. Among them, the monoclonal antibody GHRP2-88 was reactive with the extracellular domain of HGHR and the most efficient when used for immunoblotting of IM-9 cell membrane proteins.
Monoclonal antibodies against ubiquitin were prepared for the purpose of elucidating the role of hGHR-bound ubiquitin.
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