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Studies on the Regulation of Immune Functions by Growth Hormone.

Research Project

Project/Area Number 02671027
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Biological pharmacy
Research InstitutionNational Institute of Hygienic Sciences

Principal Investigator

TERAO Tadao  National Institute of Hygienic Sciences, Division of Drugs, Director, 薬品部, 部長 (60012605)

Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1991: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1990: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsGrowth hormone / Growth hormone receptor / IM-9 cells / Monoclonal antibodies / Ubiquitin / Down-regulation / Phorbol diesters / Phosphorylation / IMー9 / 抗成長ホルモン受容体抗体 / ハイブリド-マ / エビキチン / 抗ユビキチン抗体
Research Abstract

Growth hormone-dependent proliferation of IM-9 cells under low-serum conditions was inhibited by the addition of phorbol diesters. The phorbol diesters were found to down-regulate the growthhormone receptor levels. These regulations by phorbol diesters were inhibited by staurosporine or sphingosine, suggesting the involvement of C-kinases. It was also found that phosphorylation of a 55 kDa protein, which was associated with HGHR, was increased.
Monoclonal anti-peptide antibodies against human growth hormone receptor(HGHR)were prepared and characterized. Among them, the monoclonal antibody GHRP2-88 was reactive with the extracellular domain of HGHR and the most efficient when used for immunoblotting of IM-9 cell membrane proteins.
Monoclonal antibodies against ubiquitin were prepared for the purpose of elucidating the role of hGHR-bound ubiquitin.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] K.Suzuki,et al.: "Human Growth Hormone-Stimulated Growth of Human Cultured Lymphocytes (IM-9) and Its Inhibition by Phorbol Diesters through Down-Regulation of the Hormone Receptors." J.Biol.Chem.265. 11320-11327 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y.Saito,et al.: "Production and Characterization of Monoclonal Anti-Peptide Antibodies Specific for Human Growth Hormone Receptor."

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Suzuki, K. et al.: "Human Growth Hormone-Stimulated Growth of Human Cultured Lymphocytes (IM-9) and Its Inhibition by Phorbol Diesters through Down-Regulation of the Hormone Receptors." J. Biol. Chem.265. 11320-11327 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Saito, Y. et al.: "Production and Characterization of Monoclonal Anti-Peptide Antibodies Specific for Human Growth Hormone Receptor."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshiro Saito: "Production and Characterization of Monoclonal Anti-Peptide Antibodies Specific for Human Growth Hormone Receptor."

    • Related Report
      1991 Annual Research Report
  • [Publications] Suzuki,K.: "Human Growth Hormoneーstimulated Growth of Human Cultured Lymphocytes(IMー9)and Its Inhibition by Phorbol Diesters Through Downーregulation of the Hormone Receptors" J.Biol.Chem.265. 11320-11327 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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