| Co-Investigator(Kenkyū-buntansha) |
MORIGUCHI Masahide Meiji Seika Kaisha, Ltd., Pharmaceutical research Center, Toxicology Laboratory,, 薬品総合研究所・安全性研究所, 係長
MARUYAMA Hiroko Kitasato University School of Hygienic Sciences, Assistant, 衛生学部, 助手 (50129269)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1991: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1990: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Research Abstract |
The present study was initiated to determine how the distribution of orally given[G- ^3H]・7, 12dimethylbenz(a)anthracene(DMBA)into rats would change with a diet containing kelp or laver, both rich in dietary fiber. Female SD rats, aged 5 weeks, were fed a diet with 2% powdered Laminaria religiosa, or powdered sheet of Pophyra sp., or 1.5% cellulose powder for 4 days, and control animals were fed a basic diet. Then, [G- ^3H]・DMBA, 44.4 kBq/0.1 ml of olive oil, was given intragastrically into each rat. The animals were autopsied 3 and 24 hrs after dosing. Contents of[ ^3H] in organs, tisuues and blood were determined using a scintillation counter, then, contents of DMBA were calculated. As-the results, content of DMBA in the skin(target tissue)and blood of kelp-fed rats in each time point was remarkably less than that of control animals. In the laver-fed or cellulose-fed rats, attenuation of DMBA content in the skin and blood was observed 3 hrs after doding. In addition, determination of DMBA binding DNA in the skin of kelp-fed rats was carried out and resulted remarkably low value(0.0192 pmol/mg DNA), compared-with that of controls(0-3514 pmol/mg DNA).
Judging from the above-mentioned results, it was concluded that the anticarcinogenic activity of dietary seaweeds, such as kelp and laver, may partly be due to their function as a dietary ciber, attenuating the absorption of carcinogen through intestinal tract and thereby minimizing the amount of the carcinogen to reach the target tissue.
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