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Stable production of recombinant human sperm immobilizing antibody and its Fab fragment using cDNA expression vectors

Research Project

Project/Area Number 02671043
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 医学一般
Research InstitutionHyogo Medical College

Principal Investigator

YAMASAKI Noriyuki  Hyogo Medical College, Dept. Obstetrics & Gynecology, Assistant, 医学部, 助手 (50174644)

Co-Investigator(Kenkyū-buntansha) KOMORI Shinji  Hyogo Medical College, Dept. Obstetrics & Gynecology, Assistant, 医学部, 助手 (60195865)
SAWAI Hideaki  Hyogo Medical College, Dept. Obstetrics & Gynecology, Assistant, 医学部, 助手 (80215904)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1991: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1990: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordssperm immobilizing antibody / recombinant antibody / cDNA expression / gene transfection / BPV vector
Research Abstract

Sperm immobilization antibodies present in sterile women are one of theprincipal immunological causes of infertility. To identify the antigenseliciting these antibodies, we have constructed cell lines that stably secrete recombinant human sperm-immobilizing antibody using cDNA expression vectors. For production of the antibody, cDNAs of the heavy and light chain immunoglobulingenes were cloned from the class-switched (from IgM to IgG) human sperm-immobilizing antibody En46 and inserted into modified bovine papilloma virus-based cDNAexpression vectors BCMGSHyg and BCMGSNeo, respectively. These constructs were sequentially transfected into the mouse myeloma cell line X63Ag8.653 by electro-poration. The established transfectants produced recombinant antibody that retained human sperm-immobilizing activity in non-selective medium for more thanone month. The use of this genetically engineered antibody should contribute to the understanding and treatment of immunological infertility and to the development of a contraceptive vaccine.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (2 results)

All Other

All Publications (2 results)

  • [Publications] K.ベクタ"Stable Production of Recombinant Human Sperm Immobilizing Antibody using cDNA Expression Vectors"

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hideaki Sawai, Noriyuki Yamasaki, Shinji Komori, Koji Koyama, Shinzo Isojima: "Stable Production of Recombinant Human Sperm Immobilizing Antibody using cDNA Expression Vectors"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary

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Published: 1990-04-01   Modified: 2016-04-21  

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