| Project/Area Number |
02671060
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
応用薬理学・医療系薬学
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| Research Institution | Tokyo Metropolitan Institute of Medical Science. |
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Principal Investigator |
HAZATO Tadahiko The Tokyo Metropolitan Institute of medical Scince・Cancer Therapeutics, Principal Scientist, 化学療法研究部, 研究員 (60109949)
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| Co-Investigator(Kenkyū-buntansha) |
KAYA Keiji Juntendo University School of Medicine Anesthesiology, Professor, 麻酔科, 教授 (20124969)
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| Project Period (FY) |
1990 – 1992
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1991: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1990: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Spinorphin / Spinal cord / Hemorphin / Enkephalin degrading enzyme inhibitor / 分解酵素阻害物質 / Enkephalin分解酵素阻害物質 |
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| Research Abstract |
We isolated a potent inhibitor for enkephalin degrading enzymes from the bovine spinal cord, and determined its amino-acid sequence and pharmacological action. This new substance is composed of heptapeptide (Leu-Val-Val-Tyr-Pro-Trp-Thr), and has been named by us 'Sinorphin". This present study is to deterain the opioid activity and the existance of Spinorphin degrading enzyme in human spinal cord. The intracisternally injected Spinorphin produced analgesic effects in a dose -dependent manner, in dose of 100-200nnol/mouse. The analgesic effect reached the maximum within 5min and dissapeared afterl5-6Onin. Spinorphin also produces a dose-related inhibition of electrically evoked contractions of the mouse vas deferens and the guinea pig ileum. These inhibitory effects could be completely antagonised bynaloxone. The diiscovery in the bovine spinal cord of endogenoue heptapeptide, Spinorphin with potent opioid activity raises a number of pertinent questions which cannot be adequately dealt with in this study. It will now be possible, however, to test the very hypothesis that this petides act as neurotransmitters or neuromodulations at synaptic junction.
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