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Modifications of Alkaline Phosphatase Molecules by the Glycan PI-Anchor and Their Applications for Pathological Discrimination

Research Project

Project/Area Number 02671072
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Laboratory medicine
Research InstitutionSaitama Medical School

Principal Investigator

KOMODA Tsugikazu  Saitama Medical School, School of Medicine, Associate Professor, 医学部, 助教授 (10049835)

Co-Investigator(Kenkyū-buntansha) KOYAMA Iwao  Saitama Medical School, Junior college, Associate Professor, 短期大学・臨床検査学科, 助教授 (30153688)
SAKAGISHI Yoshikatsu  Saitama Medical School, School of Medicine, Full Professor, 医学部, 教授 (90049768)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1991: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1990: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsAlkaline Phosphatase / GPI-anchor / PIPLC / Diabetic rats / Intestinal enzyme / Stearate / Modification / Susceptibility / ペプチド-アンカ- / Kasaharaアイソザイム / 癌化
Research Abstract

It has been revealed that the C-terrminal amino acids of Alkaline phosphatase (ALP) molecules, except for the intestinal ALP, link to glycanphosphoinositol (GPI) involving ethanolamine and 1, 2-diacylglycerol of the GPI inserts to the biomembrane. In this regard, human placental ALP is released from the plasma membrane by phosphatidylinositol-specific phospholipase C (PIPLC), while the rat intestinal ALP is not released by this treatment (seetharam, B. ET AL. : arch. biochem. biophys., 253 : 189-l98, 1987). The fact suggests that the manner in which the GPI anchors with these ALPS is different.
With the streptozotocin-induced diabetic rats, the major ALP detected in serum was intestinal ALP. In vitro, ALP was readily released from the duodenum by piplc, but little if any was released from the ileum. The characteristics of serum ALP in rats is similar to the ileal ALP, suggesting that the circulating ALP is derived from the ileum. As reported with the IGG receptor, therefore the intestinal ALP from rats exist in both peptide-anchored and GPI-anchored forms. Other possibility, the 11eal enzyme may bears the acylated form, as human cholineesterase.
On the other hand, rabbit kidney have predominantly intestinal ALP, unlike humans. The intestinal ALP in rabbit kidney was clearly released by PIPLC treatment, like the duodenal ALP in rats. Chemical Analyses revealed that the purified intestinal ALP contains palmitate, stearate, and inositol. Molar ratios of three components were calculated to be 1 MOL, respectively. The stearate content of intestinal ALP from methylnitrosourea-induced cancer tissues of rats was calculated to be 2 MOL, the intestinal ALP in the cancer tissues was resistant to PIPLC treatment, indicating that modification of the GPI moiety in intestinal ALP molecules may alter the susceptibility to PIPLC.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] S.Unakami,T.Komoda,Y.Sakagishi: "Translocation of intestinal alkaline phosphatase in streptozotocininduced diabetic rats." International Journal of Biochemistry. 22. 1325-1331 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 坂井 隆夫,牧田 明,近藤 朝明,滝川 一,三宅 和彦,山中 正己,菰田 二一,坂岸 良克: "Phosphatidylinositolーspecific phospholipase C処理した血清アルカリ性ホスファタ-ゼアイソザイムのpolyacrylamideーgel disc電気泳動法による検討" 医学のあゆみ. 155. 741-742 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y.Fujimori-Arai,I.Koyama,K.Hirano,Y.Sakagishi,T.Komoda: "Purification and partial characterization of intestinal-like alkaline phosphatase in rabbit kidney." Archives of Biochemistry and Biophysics. 284. 320-325 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T.Sakai,N.Hirota,T.Yokoyama,T.Komoda: "Ultracytochemical and biochemical investigations of alkaline phosphatase and 5'-nucleotidase activities in carcinogen-induced well-and poorly-differentiated agenocarcinoma of the rat stomach." Acta Histochemica et Cytochemica. 24. 301-314 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] A.Nagata,N.Hirota,T.Sakai,M.Fujimoto,T.Komoda: "Molecular nature and possible presence of a membranous glycan-phos-phatidylinositol anchor of CA 125 antigen." Tumor Biology. 12. 279-286 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] I.Koyama,Y.Fujimori Arai,N.Hirota,T.Sakai,Y.Sakagishi,T.Komoda: "Liver-like alkaline phosphatase in the tissue-unspecific type enzyme found in rabbit organs." Biochimica et Biphysica Acta. 1080. 165-172 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 菰田 二一,三浦 雅一,小山 岩雄(分担): "電気泳動法のすべて(Medical Technology別冊)" 医菌薬出版株式会社(桜林郁之介,熊坂一成編), 9 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S. UNAKAMI, T. KOMODA, Y. SAKAGISHI: "Translocation of Intestinal Alkaline Phosphatase in Streptozotocin-in-Duced Diabetic Rats." International Journal of Biochemistry. 22. 1325-1331 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. SAKAI, A. MAKITA, T. KONDOH, H. TAKIGAWA, K. MIYAKE, M. YAMANAKA, T. KOMODA, Y. SAKAGISHI: "SEPARATION OF SERUM ALKALINE PHOSPHATASE ISOZYMES TREATED WITH PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE C BY POLYACRYL-AMIDE-GEL DISC ELECTROPHORESIS (IN JAPANESE)." Igaku No Ayumi. 155. 741-742 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y. FUJIMORI-ARAI, I. KOYAMA, K. HIRANO, Y. SAKAGISHI, T. KOMODA: "Purification and Partial Characterization of Intestinal-like alkaline Phosphatase in Rabbit Kid-Ney." Archives of Biochemistry and Biophysics. 284. 320-325 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. SAKAI, N. HIROTA, T. YOKOYAMA, T. KOMODA: "Ultracyto-chemical and Biochemical Investigations of Alkaline Phosphatase and 5'-Nucleotidase Activities in carcinogen-in-duced well-and Poorly-differentiated Adenocarcinoma of the Rat Stomach." Acta Histochemica et Cytochemica. 24. 301-414 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] A. NAGATA, N. HIROTA, T. SAKAI, M. FUJIMOTO, T. KOMODA: "Molecular Nature and Possible Presence of a Membranous Glycan-phosphatidylinositol Anchor of CA 125 Anchor." Tumor Biology. 12. 279-286 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] I. KOYAMA, Y. FUJIMORI-ARAI, N. HIROTA, T. SAKAI, Y. SAKAGISHI, T. KOMODA: "Liver-like Alkaline Phosphatase in the Tissue-unspecific Type Enzyme Found in Rabbit Organs." Biochimica et Biophysica Acta. 1080. 165-172 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. KOMODA, M. MIURA, I. KOYAMA: "Alkaline Phosphatase Isoenzymes." A Review of Electrophoretic Techniques (in Medical Techmology). Ishiyaku press. 101-109 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T.Irino,M.Matsushita,T.Komoda,Y.Sakagishi: "Apparent alkaline phosphatase activity in serum measured with phosphorylcholine as substrate" Clinical Chemistry. 37. 479 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Sakai,N.Hirota,T.Yokoyama,T.Komoda: "Ultracytochemical and biochemical investigations of alkaline phosphatase and 5^1ーnucleotidase activities in carcinogenーinduced well and poorlyーdifferentiated adenocarcinoma of the rat stomach." Acta Histochemica et Cytochemica. 24. 301-314 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] A.Nagata,N.Hirota,T.Sakai,M.Fujimoto,T.Komoda: "Molecular nature and possible presence of a membranous glycanーphosphatidylinositol anchor of CA 125 antigen." Tumor Biology. 12. 279-286 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] I.Koyama,Y.FujimoriーArai,N.Hirota,T.Sakai,Y.Sakagishi,T.Komoda: "Liverーlike alkaline phosphatase in the tissueーunspecific type enzyme found in rabbit organs." Biochimica et Biophysica Acta. 1080. 165-172 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Komoda,I.Koyama,Y.FujimoriーArai,F.Yamagishi,Y.Sakagishi,N.Hirota: "Lectin affinity chromatography as a useful tool for determining the heterogeneity of alkaline phosphatase:intestinal like enzyme." Proceedings of AsianーPacific Congress of Clinical Biochemistry,Elsevier Scientific Co.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 菰田 二一,三浦 雅一,小山 岩雄(分担): "電気泳動法のすべて(Medical Technology別冊)" 医歯薬出版株式会社(桜林郁之介,熊坂一成編), 9 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Unakami,S.,Komoda,T.,Sakagishi,Y.: "Translocation of intestinal alkaline phosphatase in streptozotocininduced diabetic rats." Int.J.Biochem.22. 1325-1331 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Arai,Y.,Koyama,I.,Hirita,N.,Sakai,T.,Sakagishi,Y.,Komoda,T.: "Developmental changes in the antigenicity and sugar chain heterogeeity of rabbit alkaline phosphatases." Int.J.Biochem.22. 143-152 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 坂井 隆夫,牧田 明,近藤 朝明,滝川 一,三宅 和義,山中 正己,菰田 二一,坂岸 良克: "phosphatidylinositolーspecitic phospholipase C処理した血清アルカリ性ホスファタ-ゼアイソザイムのpolyacrylamideーgel disc 電気泳動法による検討" 医学のあゆみ. 155. 741-742 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nagata,A.,Miura,M.,Komoda,T.: "Sandwich assay for carcinoembryonic antigen with immobilized lectins and a monoclonal antibody." Tumor Biol.12. 35-44 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] FujimoriーArai,Y.,Koyama,I.,Hirano,K.,Sakagishi,Y.,Komoda,T.: "Purification and partial characterization of intestinalーlike alkaline phosphatase in rabbit kidney." Arch.Biochem.Biophys.284. 320-325 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Iino,T.,Matsushita,M.,Komoda,T.,Sakagishi,Y.: "Apparent alkaline phosphatase in serum measured with phosphorylcholine as substrate." Clin.Chem.(1991)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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