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Study of growth hormone receptor abnormalities in short children

Research Project

Project/Area Number 02671112
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 内分泌・代謝学
Research InstitutionTokyo Women's Medical College

Principal Investigator

HIZUKA Naomi  Tokyo Women's Medical College, Associate Professor, 医学部, 助教授 (80147397)

Co-Investigator(Kenkyū-buntansha) YASUMOTO Kumiko (ASAKAWA Kumiko)  Tokyo Women's Medical College, Clinical Associate, 医学部, 助手 (80151017)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1991: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1990: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsgrowth hormone (GH) / GH receptor / GH binding protein (GHBP) / short stature / 成長ホルモン
Research Abstract

Recently it has been reported that serum GH binding protein (GHBP) is a extracellular hormone binding domain of the GH receptor. Therefore, the measurement of serum GHBP may be useful to evaluate GH receptor in vivo. At present, gel filtration method is used for measurement of serum GHBP, but by this method multiple samples could not be measured simultaneously. We have developed a simple method for measurement of serum GHBP using anti-GH receptor antibody. Binding of ^<125>I-hGH to human serum GHBP was immunoprecipitated with monoclonal anti-GH receptor antibody (GHRAB : * 263 ; AGEN Biomedical Ltd, QLD, Australia) that did not inhibit hGH binding to human serum GHBP. Serum GHBP in various clinical conditions was measured by this method, and the results were expressed'as percent specific binding relative to an adult reference serum. Serum GHBP values in normal adult males, normal adult females, patients with GH deficiency and patients with acromegaly were 87.5<plus-minus>9.7, 74.3<plus-minus>5.3, 106.3<plus-minus>5.2, and 35.4<plus-minus>4.4%, respectively, suggesting that GHBP is regulated by endogenous GH. Scatchard analysis of the bindings, carried out with correction made for endogenous serum GH, revealed that changes of the binding were mainly due to the changes of binding capacity. Furthermore, the serum GHBP values in patients with liver cirrhosis and poor nutritional conditions, and in some short children and those in late pregnant women and patients with ossification of the posterior longitudinal ligament (OPLL) were elevated From the above data, we conclude that this method could be practical to evaluate GH receptor in various clinical conditio

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Asakawa K.et al: "Radioimmunoasay for insulin-like growth fador II(IGF-II)." Endocrinol.Japan.37. 607-614 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takano K.et al.: "Effect of Short-term growth hormore therapy in short children without growth hormone deficiency." Acta Paediatr.Scand. 366(Suppl). 14-22 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takano K.et al.: "Repeated sc administration of recombinant human insulin-like growth factor I(IGF-I) to human subjects for 7 days." Growth Regulation. 1. 23-26 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hizuka N.et al.: "Measurement of free form of insulin-like growth factor I in human plaasme." Growth Reguration. 1. 51-55 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hizuka N.et al.: "Single sc administration of insulin-like growth factor I(IGF-I) in normal men." Advances in Experimental Medicune and Biology. 193. 105-112 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hizuka N.et al.: "Modern Concepts of Insulin-Like Growth Factors-Administration of insulin-like growth factors in normal adults,patients with growth hormone deficiency and rats." Elsevier, 607-615 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Asakawa K. et al.: "Radioimmunoassay for insulin-like growth factor II (IGF-II)." Endocrinol. Japan.37. 607-614 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takano K. et al.: "Effect of short-term growth hormone therapy in short children without growth hormone deficiency." Acta Paediatr Scand. 366 (Suppl). 14-22 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takano K. et al.: "Repeated sc administration of recombinant human insulin-likd growth factor I (IGF-I) to human subjects for 7 days." Growth Regulation. 1. 23-26 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hizuka N. et al.: "Measurement of free form of insulin-like growth factor I in human plasma." Growth Regulation. 1. 51-55 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hizuka N. et al.: "Single sc administration of insulin-like growth factor I (IGF-I) in normal men." Advances in Experimental Medicine and Biology. 193. 105-112 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hizuka N. et al.: "Administration of insulin-like growth factors in normol adults, patients with growth hormone deficienty ad rats. In Spence EM (ed) Modern Concepts of Insulin-Like Growth Factors" Elsevier, New York. 607-615 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takano K.et al.: "Repeated sc administration of recombinant human insulinーlike growth factor I(IGFーI)to human subjects for 7 days." Growth Regulation. 4. 23-26 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hizuka N.et al: "Measurment of free form of insulinーlike growth factor I in human plasma." Growth Regulation. 1. 51-55 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hizuka N.et al.: "Single sc administration of insulinーlike growth factor I(IGFーI)in normal men." Advances in Experimental Medicine and Biolosy. 193. 105-112 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hizuka N.et al.: "Modern Concepts of InsulinーLike Growth FactorsーAdministration of insulinーlike growth factors in normal adults,patient growth hormone deficiency and rats." Elsevier, 607-615 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Asakawa K.,et al.: "Radioimmunoassay for insulinーlike growth factor II(IGFーII)" Endocrinol. Japon. 37. 607-614 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hizuka N.,et al.: "Measurement of free from of insulinーlike growth factor I in human plasma." Growth Regulation. 1. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 肥塚 直美他: "成長ホルモン(GH)受容体抗体を用いた血中GH結合蛋白の測定" 日本内分泌学会雑誌. 66. 306 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 福田 いずみ他: "低身長者の血中成長ホルモン(GH)結合蛋白の測定" 日本内分泌学会雑誌. 66. 896 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hizuka N.,et al.: "Cellular and Molecular Biology of Insulin and IGF:Single sc administration of insulinーlike growth factor I (IGFーI)in normal men." Pleum Press, (1991)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2021-09-29  

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