| Project/Area Number |
02671123
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Hematology
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| Research Institution | The Institute of Medical Science, The University of Tokyo |
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Principal Investigator |
SATO Noriharu The Institute of Medical Science Associate Professor, 医科学研究所, 助教授 (90162461)
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| Co-Investigator(Kenkyū-buntansha) |
ASANO Shigetaka The Institute of Medical Science Professor, 医科学研究所, 教授 (50134614)
TANI Kenzaburo The Institute of Medical Science Assistant Professor, 医科学研究所, 講師 (00183864)
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| Project Period (FY) |
1990 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1991: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1990: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Alkaline phosphatase / Neutrophil / Molecular Biology / アルカリフォスタファタ-ゼ / プロモ-タ- / 発現誘導 |
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| Research Abstract |
We have Inolecularly cloned a DNA fragment that contains the promoter sequence of liver/bone/kidney-type alkaline phosphatase gene(Alp). According to the report by Weiss et al., we isolated a DNA fragment of 672 bp that was created by digestion with the restriction enzyme Ava II, and made a chimeric reporter plasmid containing Alp promoter and upstream promoter elements ligated to CAT gene.
More recently, Alp gene was found to have two alternative promoters that make dif-L9erent mRNAs. This raised a urgent problem that we must know which promoter is really active in neutrophils in order to examine the Alp induction in neutrophils.
Since the mRNAs produced by the alternative promoters of Alp differ only in exon I sequence, we made primers specific to each exon I sequence and common exon II sequence and amplified the cDNA produced by MRNA of neutrophils. The results suggested that neutrophils predominantly use bone-type promoter.
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