Analysis of Sweat by High Performance Liquid Chromatography

Research Project

Project/Area Number	02680055
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	家政学
Research Institution	Ochanomizu University
Principal Investigator	OGAWA Shojiro Ochanomizu Univ., Home Econ., Prof., 家政学部, 教授 (20013196)
Project Period (FY)	1990 – 1991
Project Status	Completed (Fiscal Year 1991)
Budget Amount *help	¥1,900,000 (Direct Cost: ¥1,900,000) Fiscal Year 1991: ¥500,000 (Direct Cost: ¥500,000) Fiscal Year 1990: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywords	Sweat / High performance liquid chromatography / Amino acid
Research Abstract	The purpose of this research is to analyze components of sweat by way of high performance liquid chromatography (HPLC) technique, and to investigate the influences of sweat on clothing materials. In this investigation, main attention was given to the analysis of amino acids contained in the sweat, because amino acids have been found to affect the photodegradation of dye. In the first place analytical conditions to estimate amino acids contents were determined by using mixed amino acids standard solution. The pre-columm technique was applied to amino acid component labeled with 0-Phthalaldehyde and 2-mercaptoethanol. Advisable conditions are as follows : wave length : 350 nm, column : silica-ODS, eluent : aetonitrile/methanol/sodium acetate buffer solution. Under these conditions, 12 amino acids in the sweat were able to be detected. It was found that serine was contained in the largest amount in the sweat. When wool, silk, nylon and acryl fibers were immersed in the sweat, ratio of amino acid composition changed and the quantity of ammonia increased, on the other hand, cotton and polyester fibers little affected the composition of the sweat.