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Mechanisms of Radiation-Induced Apoptosis

Research Project

Project/Area Number 02680176
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 放射線5生物学
Research InstitutionNational Institute of Radiological Sciences (NIRS)

Principal Investigator

OHYAMA Harumi  NIRS, Division of Radiation Health, Sinior Research Official, 障害臨床研究部, 主任研究官 (70160645)

Co-Investigator(Kenkyū-buntansha) MITA Kazuei  NIRS, Division of Biology. Senior Research Official, 生物研究部, 主任研究官 (30159165)
YAMADA Takeshi  NIRS, Division of Biology, Division Head, 生物研究部, 室長 (30166714)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1991: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1990: ¥900,000 (Direct Cost: ¥900,000)
KeywordsApoptosis / Radiation-induced cell death / Thymocytes / Thymic lymphoma / Radiation sensitivity / Chromatin DNA fragmentation / Ubiquitin gene expression / apoptosis / 放射線誘発細胞死 / 細胞生存曲線 / ユビキチン / DNA断片化
Research Abstract

Thymocytes show typical apoptosis within a few hours following irradiation. Analysis of proteins with two deminsional gel electrophoresis revealed that a few proteins changed associated with the cell death. Inhibitors of protein synthesis and proteinase suppressed the cell death. Threfore, it is supposed that protein synthesis as well as protein degradation playsarole in the development of apoptosis. Ubiquitin gene expression increased slightly in the earlier phase and decreased thereafter in progression of the cell death. Chromatin DNA fragmentation into oligonucteosomal units represents the most characteristic biochemical event in apoptosis. We established microgel electrophoresis technique to detect the DNA cleavage. Results revealed that apoptotic cells showed similar migration pattern with a tear-drop shaped fragmented DNA tail and non-migrating DNA core particle, while viable cells exhibited no DNA migration. The findings indicate that apoptotic breakage of DNA in X-irradiated thymocytes is an "all or none" type event. In the present study, we found several extremely radiosensitive thymic lymphoma cell lines establised in our institute. These cells showed typical apoptosis within a few hours following low doses X-irradiation. Characteristic DNA fragmentation could be detected both by micro and macro get electrophoresis of DNA. Colony forming ability decreased with low doses X-irradiation. Further study on the mechanism of radiation induced apoptosis is now in progress.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] 大山 ハルミ他: "Detection of apoptotic DNA fragmentation in individual Xーirradiated thymocytes" Radiat.Res.A twentiesーcentury perspective:Proc.9th Int.Con.Radiat.Res.308 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 耿 勇志他: "放射線自爆死(apoptosis)に伴うクロマチン断片化のミクロゲル電気泳動による検出" 日本医学放射線学会生物部会誌. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 大山 ハルミ他: "細胞自爆死に伴うクロマチン断片化" 組織培養研究. 10. 103 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 三田 和英他: "Essential Factors Determining Codon Usage in Ubiquitin Genes" J.Mol.Evol.33. 216-225 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 根井 充他: "Evolutionaly conserved structure of the 3'nonーtranslated region of a Chinese hamster polyubiquitin gene." Biochim.Biophys.Acta.(1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 三田 和英他: "遺伝子におけるコドン選択の不均一性" 蛋白質、核酸、酵素. 35. 2011-2021 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 根井 充他: "色田 幹雄監修 放医研シンポジウム 放射線抵抗性の誘導“放射線によるユビキチン遺伝子の発現誘導"" 放射医学総合研究所, (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H. Ohyama: "Detection of apoptotic DNA fragmentation in individual X-irradiated thymocytes. : Radiation Research. A twentieth-century perspective : Proceeding of 9 th International Congress of Radiation Research" 308 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y. Geng: "Detection of radiation-induced apoptotic chromatin DNA fragmentation by microgel electrophoresis. (Japanese)"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H. Ohyama: "Chromatin DNA degradation associated with apoptosis. Tissue Culture Research Communications, 10, 103 (Japanese)" (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K. Mita: "Essential Factors Determining Codon Usage in Ubiquitin Genes." Journal of Molecular Evolution.33. 216-225 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M. Nenoi: "Evolutionaly conserved structure of the 3 non-translated region of a Chinese hamuster polyubiquitin gene. Biochemica Biophysica Acta"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Yamada: "Radiation-induced cell death of rat thymocytes is internally programmed. US-Japan Cancer Meeting "Programmed Cell Death and T Cell Repertoire Selection". Fukuoka, 1992, 2."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H. Ohyama: "Methods Used for Analyzing Morphological and Biochemical Characteristics of Radiation-induced Apoptosis. US-Japan Cancer Meeting "Programmed Cell Death and T Cell Repertoire Selection". Fukuoka, 1992, 2"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H. Ohyama, M. Muto & T. Yamada: "Apoptosis of newly established radiosensitive mouse lymphoma cell lines. The International Conference on Low Dose Irradiation and Biological Defense Mechanisms. Kyoto, 1992, 7"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 大山 ハルミ他: "Detection of apoptotic DNA fragmentation individual Xーirradiated thymocytes" Radiat.Res.,A Twentiethーcentury persprctive:Proc.9th Int.Cong.Radiat.Res.308 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 三田 和英他: "Essential Factors Determining Codon Usage in Ubiquitin Genes" J.Mol.Evol.33. 216-225 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 大山 ハルミ: "Characterization of apoptotic DNA fragmentation in individual Xーirradiated thymocytes." Annual Report of Natl.Inst.Radiol.Sci.35 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 根井 充他: "Evolutionally conserved structure of the 3'nonーtranslated region of Chinese hamster polyubiquitin gene." Biochim.Biophys.Acta. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 耿 勇志他: "放射線自爆死(apoptosis)に伴うクロマチン断片化のミクロゲル電気泳動法による検出" 日本医学放射線学会生物部会誌. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 大山 ハルミ他: "細胞自爆死に伴うクロマチン断片化" 組織培養研究. 10. 103 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] K.Mita,S.Ichimura and M.Nenoi: "Essential factors determining codon usage in ubiquitin genes" J.Molecular Evolution.

    • Related Report
      1990 Annual Research Report
  • [Publications] S.Ichimura,K.Mita and M.Nenoi: "Developmental expression of ubiquitin genes in posterior silk gland of Bombyx mori" J.Cell Biology. 111. 123 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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