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Approaches with Arabidopsis thaliana Mutants to the Regulatory Genes for Photosynthesis

Research Project

Project/Area Number 02804056
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 植物生理学
Research InstitutionUniversity of Shizuoka (1991)
Nagoya University (1990)

Principal Investigator

KOBAYASHI Hirokazu  Univ. Shizuoka, Graduate Sch, tutritional Environmental Sci., Associate professor, 大学院・生活健康科学研究科, 助教授 (80170348)

Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1991: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1990: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsPhotosynthesis Genes / Regulatory Genes / Mutants / Arabidopsis thaliana / RuBisCO / rbcS / cab / PCR
Research Abstract

A. thaliana is most suitable for handling in the aspect of molecular generics to thoroughly Investigate mechanisms goveming the transcriptional regulation.
1. A Model Experiment for Screening Photosynthetic Mutants
A. thaliana grew on the agar medium of Gamborg B5 in supplementation with glucose or sucrose even in the presence of the inhibitor of photosynthetic electron transport, 3-(3, 4-dich ; orophenyQ-1, 1-dimethylurea(DCMU), although it did not grow without sugar sources under that condition. The resulting A. thaliana grown with inhibited level of photosynthetic activity, generated high red fluorescence upon exposure to a long wave UV light.
2. Mutagenesis of A thaliana transformed with Reporter and Suicide Gones
Genes for P-glucuronidase(GUS)and chloramphenicol acetyltransferase(CAT)as reporter genes, as well as a gene for alcohol dehydrogenase(ADH)as a suicide gene, were tried to introduce into A. thaliana prior to mutagenesis with ethylmethanesuffonate(EMS). This strategy may promi … More se to make the selection of objective mutants easily in combination with screening by high red fluorescence. We have focused on members of genes for small subunit of ribulose 1, 5 -bisphosphate carboxylase/oxygenase(RuBisCO, rbcS-3B)and chlorophyll a/binding proteins(cabl). Polymerase chain reaction(PCR)with thermostable DNA polymerases associated with a 3'-5' proofreading exonuclease activity, was used to quickly obtain approximately 1.5-kilobase pair(kbp)DNA fragments covering promoters and their upstream regions of rbcS-38and cabl, as nucleotide sequences of these genes have been published. A gene for ADH(approximately 2.2 kbp)was similarly amplilied from A. thaliana DNA. The fragments possessing the promoter and regulatory activities of rbcS-3B and cabl were-placed upstream of genes for GUS, CAT, and ADH, and tried to introduce Into A. thaliana(Columbia and Bensheim, adhl. The transgenic A. thaliana is mutagenized with EMS, and mutants which less express rbcS-3B or cabl in green leaves is being screened. Less

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (35 results)

All Other

All Publications (35 results)

  • [Publications] J.Naernprasirtsiri,and H.Kobayashi: "Application of an efficient strategy with a phoge λ vector for constructing a physical map of the amyloplast genome of sycamore(Acer pseudoplatanus)" Archives of Biochemistry and Biophysics. 276. 172-179 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H.Kobayashi,J.Ngernprasirtsiri,and T.Akazawa: "Transcriptional regulation and DNA methylation in plastids during transitional conversion of cholroplasts to chromoplasts" EMBO Journal. 9. 307-313 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] A.M.Viale,H.Kobayashi,and T.Akazawa: "Distinct Properties of Escherichia coli products of plant-type ribulose-1,5-bisphosphate carboxylase/oxygenase directed by two sets of genes form the photosynthetic bacterium Chromatium vinosum" The Journal of Biological chemistry. 265. 18386-18392 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] J.Ngernprasirtsiri,H.Kobayashi,and T.Akazawa: "Expression of photosyntheric genes is distinctly different between chlorplasts and amyloplasts in liquid-cultured cells of Sycamore(Acer pseudoplatanus L.)" Cell Structure and Function. 15. 273-283 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] J.Ngernprasirtsiri,H.Kobayashi,and T.Akazawa: "DNA methylation is a determinative element of photosynthesis gene expreesion in amyloplasts from liquid-cultured cells of Sycamore(Acer pseudoplatanus L.)" Cell Structure and Function. 15. 285-293 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H.Kobayashi,A.M.Viale,T.Takabe,T.Akazawa,K.Wada,K.Shinozaki,K.Kobayashi,and M.Sugiura: "Sequence and expressionfof genes necoding the large and small subunits of ribulose 1,5-bisphosphate carboxylase/oxygenease from Chromatium vinosum" Gene. 97. 55-62 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 杉本 達夫,仲本 準,小林 裕和: "「生物化学実験法シリ-ズ」光合成酵素系の研究法:細胞から遺伝子まで" 学会出版センタ-,

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] J. Ngernprasirtsiri, and H. Kobayashi: "Application of an efficient strategy with a phage lambda vector for constructing a physical map of the amyloplast genome of sycamore (Acer pseudoplatanus)" Arch. Biochem. Biophys.276. 172-179 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H. Kobayashi, J. Ngernprasirtsiri, and T. Akazawa: "Transcriptional regulation and DNA methylation in plastids during transitional conversion of chloroplasts to chromoplasts" EMBO J.9. 307-313 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] A. M. Viale, H. Kobayashi, and T. Akazawa: "Distinct Properties of Escherichia coli products of plant-type ribulose-1, 5-bisphosphate carboxylase/oxygenase directed by two sets of genes from the photosynthetic bacterium Chromatium vinosum" J. Biol. Chem.265. 18386-18392 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] J. Ngernprasirtsiri, H. Kobayashi, and T. Akazawa: "Expression of photosynthetic genes is distinctly different between chloroplasts and amyloplasts in liquid-cultured cells of Sycamore (Acer pseudoplatanus L.)" Cell Struct. Funct.15. 273-283 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] J. Ngernprasirtsiri, H. Kobayashi, and T. Akazawa: "DNA methylation is a determinative element of photosynthesis gene expression in amyloplasts from liquid-cultured cells of Sycamore (Acer pseudoplatanus L.)" Cell Struct. Funct.15. 285-293 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H. Kobayashi, A. M. Viale, T. Takabe, T. Akazawa, K. Wada, K. Shinozaki, K. Kobayashi, and M. Sugiura: "Sequence and expression of genes encoding the large and small subunits of ribulose 1, 5-bisphosphate carboxylase/oxygenase from Chromatium vinosum" Gene. 97. 55-62 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] A. M. Viale, H. Kobayashi, T. Akazawa, and S. Henikoff: "rbcR, a gene coding for a member of the LysR family of transcriptional regulators, is located upstream of the expressed set of ribulose 1, 5-bisphosphate carboxylase/oxygenase genes in the photosynthetic bacterium Chromatium vinosum" J. Bacteriol.173. 5224-5229 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H. Kobayashi: "Differentiation of amyloplasts and chromoplasts" Cell Culture and Somatic Cell Genetics of Plants, Vol. 7B : The Photosynthetic Apparatus : Molecular Biology and Operation. Academic Press, San Diego. 395-415 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H. Kobayashi: "Regulation of functional chloroplast formation by altered DNA template activity in transcription (in Japanese)" Shokubutu-Saiboukougaku (Plant Cell Technology, in Japanese). 2. 198-207 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H. Kobayashi: "Genes for photosynthesis in prokaryotes (in Japanese)" Bioscience Strategy Manual : Useful Markers, Materials and Methods (in Japanese). Kyouritu-Shuppan, Tokyo. 485-495 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H. Kobayashi, and k. Kobayashi: "Hybridization : Principles and applications (in Japanese)" Shokubutu-Saiboukougaku (Plant Cell Technology, in Japanese). 2. 543-555 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Sugiyama, H. Nakamoto, and H. Kobayashi: "A Research manual for photosynthetic Enzymes : Genes to Cells (in Japanese)" Series of Methods in Biochemical Experiments. Gakkai-Shuppan Center.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H.Kobayashi,A.M.Viale,T.Takabe,T.Akazawa,K.Wada,K.Shinozaki,K.Kobayashi,and M.Sugiura: "Sequence expression of genes encoding the large and small subunits of ribulose 1,5ーbisphosphate carboxylase/oxygenase from Chromatium vinosum" Gene. 97. 55-62 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] A.M.Viale,H.Kobayashi,T.Akazawa,and S.Henikoff: "rbcR,a gene coding for a member of the LysR family of transcriptional regulators,is located upstream of the expressed set of ribulose 1,5ーbisphosphate carboxylase/oxygenase genes in the photosynthetic bacterium Chromatium vinosum" Journal of Bacteriology. 173. 5224-5229 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] H.Kobayashi: "Differentiation of amyloplasts and chromoplasts" In Cell Culture and Somatic Cell Genetics of Plants,Vol.7B:The Photosynthetic Apparatus:Molecular Biology and Operation,edited by L.Bogorad,and I.K.Vasil,Academic Press,San Diego. 395-415 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.tsuge,and H.Kobayashi: "Molecular analysis of genes for pathogenicity of Alternaria alternata Japanese pear pathotype,a hostーspecific toxin producer" In Molecular Strategies of Pathogens and Host Plants,edited by S.S.Patil,S.Ouchi,D.Mills,and C.Vance,SpringerーVerlag Publishers,New York. 119-129 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 杉山 達夫,仲本 準,小林 裕和: "「生物化学実験法シリ-ズ」光合成酵素系の研究法:細胞から遺伝子まで" 学会出版センタ-,

    • Related Report
      1991 Annual Research Report
  • [Publications] J.Ngernprasirtsiri,and H.Kobayashi: "Application of an efficient strategy with a phage λ vector for constructing a physical map of the amyloplast genome of sycamore (Acer pseudoplatanus)" Archives of Biochemistry and Biophysics. 276. 172-179 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] H.Kobayashi,J.Ngernprasirtsiri,and T.Akazawa: "Transcriptional regulation and DNA methylation in plastids during transitional conversion of chloroplasts to chromoplasts" EMBO Journal. 9. 307-313 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] A.M.Viale,H.Kobayashi,and T.Akazawa: "Distinct Properties of Escherichia coli products of plantーtype ribuloseー1,5ーbisphosphate carboxylase/oxygenase directed by two sets of genes from the photosynthetic bacterium Chromatium vinosum" The Journal of Biological Chemistry. 265. 18386-18392 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] J.Ngernprasirtsiri,H.Kobayashi,and T.Akazawa: "Expression of photosynthetic genes is distinctly different between chloroplasts and amyloplasts in liquidーcultured cells of Sycamore(Acer pseudoplatanus L.)" Cell Structure and Function. 15. 273-283 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] J.Ngernprasirtsiri,H.Kobayashi,and T.Akazawa: "DNA methylation is a determinative element of photosynthesis gene expression in amyloplasts from liquidーcultured cells of Sycamor(Acer pseudoplatanus L.)" Cell Structure and Function. 15. 285-293 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] H.Kobayashi,A.M.Viale,T.Takabe,T.Akazawa,K.Wada,K.Shinozaki,K.Kobayashi,and M.Sugiura: "Sequence and expression of genes encoding the large and small subunits of ribulose 1,5ーbisphosphate carboxylase/oxygenase from Chromatium vinosum" Gene. 97. 55-62 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] H.Kobayashi: "Differentiation of amyloplasts and chromoplasts" In Cell Culture and Somatic Cell Genetics of Plants,Vol.7:The Photosynthetic Apparatus:Molecular Biology and Operation,edited by L.Bogorad,and I.K.Vasil,Academic Press,San Diego.

    • Related Report
      1990 Annual Research Report
  • [Publications] 小林 裕和: "DNA転写鋳型活性の変化による葉緑体機能発現の制御" 植物細胞工学. 2. 198-207 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 小林 裕和: "原核生物の光合成遺伝子" 「バイオサイエンス研究における新しい素材とマ-カ-・プロ-ブ」,瀬野悍二他編,共立出版. 485-495 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 小林 裕和,小林 京子: "ハイブリダイゼ-ション:原理と応用" 植物細胞工学. 2. 543-555 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 杉山 達夫,仲本 準,小林 裕和: "「生物化学実験法シリ-ズ」光合成酵素系の研究法:細胞から遺伝子まで" 学会出版センタ-,

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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