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Study of nuclear membrane differentiation between germ and vegetative nuclei in early development of Paramecium.

Research Project

Project/Area Number 02804063
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 動物発生・生理学
Research InstitutionYamaguchi University

Principal Investigator

FUJISHIMA Masahiro  Yamaguchi Univ. Biol. Inst. Associate Professor, 理学部, 助教授 (40127783)

Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1991: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1990: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsParamecium / Macronucleus / Nuclear membrane / Nuclear membrane antigen / Nuclear membrane differentiation / Monoclonal antibody / Nuclear differentiation / 栄養核 / 核膜特異的抗原 / 繊毛 / 接合型物質
Research Abstract

1. Isolated macronuclei of Paramecium caudatum were injected into mice, and hybridomas bet ween the spleen cells and mieroma cells (NS-1) were obtained by cell fusion. The hybridoma was screened by indirect immunofluorescence microscopy and cloned by limiting dilution. Monoclonal antibody (MAM-1) against macronuclear membrane of P. caudatum was obtained from cell-fee medium of the hybridoma cells.
2. Molecular weight of the MAM-1 antigen was ca. 70 kDa by immunoblotting.
3. Indirect immunofluorescence microscopy showed that the antigens appeared in 4 out of 8 postzygotic nuclei immediately after the 4 nuclei differentiated morphologically into the macronuclear anlagen, and the antigens were constitutionally present in the membrane through the cell cycle. The antigens were not detected in the micronucleus. The results indicate that the nuclear membranes differentiate between the two kinds of nuclei in early development.
4. Immunofluorescence microscopy on species-specificity of the cross reactivity of the antibody showed that all strains of P. caudatum and P. jenningsi examined showed the flurescence in the macronuclear membranes but not in P. bursaria and Tetrahymena thermophila and T. pyriformis examined.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] M.Fujishima: "Induction of reinitiation of meiosis in amphibian Bufo and Xenopus oocytes by injection of M-phase extracts of ciliate Tetrahymena needs the recipient protein synthesis." Experimental Cell Research. 193. 155-160 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M.Fujishima: "Sensitivity of the infectious long form of the macronuclear endosymbiont Holospora obtusa of the ciliate Paramecium caudatum against chemical and physical factors." European Journal of Protistology. 27. 119-126 (1911)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M.Fujishima: "Developmental expression of macronuclear specific antigen in Paramecium caudatum." Developmental Genetics. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M.Fujishima: "Injection of MRIF of ciliate Tetrahymena thermophil into amphibian Bufo and Xenopus oocytes induces MPF activity by dephosphorylation of the oocyte cdc2." Journal of Protozoology.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M.Fujishima: "Costimulation-induced rounding in Tetrahymena thermophila:Early cell-shape transformation induced by sexual cell-to-cell collisions between complementary mating types." Developmental Biology.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M.Hori: "Identification of ciliary membrane proteins closely related with the mating reactivity of Paramecium caudatum." Acta Protozoologica.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M. Fujishima: "Induction of reinitiation of meiosis in amphibian Bufo and Xenopus oocytes by injection of M-phase extracts of ciliate Tetrahymena needs the recipient protein synthesis." Experimental Cell Research. 193. 155-160 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M. Fujishima: "Sensitivity of the infectious long form of the macronuclear endosymbiont Holospora obtusa of the ciliate Paramecium caudatum against chemical and physical factors." European Journal of Protistology. 27. 119-126 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M. Fujishima: "Developmental expression of macronuclear specific antigen in Paramecium caudatum." Developmental Genetics.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M. Fujishima: "Injection of MRIF of ciliate Tetrahymena thermophila into amphibian Bufo and Xenopus oocytes induces MPF activity by dephosphorylation of the oocyte cdc2." Journal of Protozoology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M. Fujishima: "Costimulation-induced rounding in Tetrahymena thermophila : Early cell-shape transformation induced by sexual cell-to-cell collisions between complementary meting types." Developmental Biology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M. Hori: "Identification of ciliary membrane proteins closely related the mating reactivity of Paramecium caudatum." Acta Protozoologica.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M.Fujishima: "Induction of reinitiation of meiosis in amphibian Bufo and Xenopus oocytes by injection of Mーphase extracts of ciliate Tetrahymena needs the recipient protein synthesis." Experimental Cell Research. 193. 155-160 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] M.Fujishima: "Sensitivity of the infectious long form of the macronuclear endosymbiont Holospora obtusa of the ciliate Paramecium caudatum against chemical and physical factors." European Journal of Protistology. 27. 119-126 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] M.Fujishima: "Developmental expression of macronuclear specific antigen in Paramecium caudatum." Developmental Genetics. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] M.Fujishima: "Injection of MRIF of ciliate Tetrahymena thermophila into amphibian Bufo and Xenopus oocytes induces MPF activity by dephosphorylation of the oocyte cdc2." Journal of Protozoology.

    • Related Report
      1991 Annual Research Report
  • [Publications] M.Fujishima: "Costimulationーinduced rounding in Tetrahymena thermophila:Early cellーshape transformation induced by sexual cellーtoーcell collisions between complementary mating types." Developmental Biology.

    • Related Report
      1991 Annual Research Report
  • [Publications] M.Hori: "Identification of ciliary membrane proteins closey related with the mating reactivity of Paramecium caudatum." Acta Protozoologica.

    • Related Report
      1991 Annual Research Report
  • [Publications] M.Fujishima,Y.Inoue,T.Sawada and T.Fukumoto: "A monoclonal antibody specific for macronaclear proteins of the unicellular eukaryote Paramecium candatum and timing of the appearance of the antigens in macronuclear ditterentiation." Journal of Cell Science.

    • Related Report
      1990 Annual Research Report
  • [Publications] M.Fujishima and Y.Inoue: "Purification of ventral surface specific cilia specific antigen of ciliate Paramecium condatum" Journal of Cell Biology.

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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