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A new volatile growth factor

Research Project

Project/Area Number 02804068
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 動物形態・分類学
Research InstitutionWaseda University

Principal Investigator

NAMIKI Hideo  Waseda University, Department of Biology, Professor, 教育学部, 助教授 (50120928)

Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1991: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1990: ¥700,000 (Direct Cost: ¥700,000)
Keywordsgrowth factor / fetal calf serum / human fibroblast cell
Research Abstract

When human fibroblast cells were cultured in a multiplate either covered with a lid or, alternatively without a lid, proliferation of the cells in the plate without a lid was significantly less as compared with that in the plate with a lid. The explanation of the result may be as follows : A highly volatile growth factor (s) was secreted from the cells or bovine calf serum. We thus tried to isolate and identify the factor (s). Fetal bovine calf serum (FCS) was freez-dried, from which the volatile part was firstly trapped in an ethanol-trap (-40 C), and further trapped in a liquid nitrogen-trap (-195.8 C). Strongly smelling and highly volatile liquid was collected in the second trap. The liquid thus collected was then processed on a gasschromatography-massspectrometer (GC-MS). As a result 4 known molecules : ethanol, acetone, ammonia, and cyclohexanol were identified, however, several unidentified molecules seemed to be remained. Those four identified molecules possessed unfortunately no growth promoting effects on the cells. Unidentified molecules have been tried to processed on further steps such as a MS-MS.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] MIEKO KARIYA,HIDEO NAMIKI: "The Effect of Culture Plate Ventilation Space on Cell Growth in vitro" Zoological Science. 8. 197-199 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] MIEKO KARIYA,HIDEO NAMIKI: "The Effect of Culture Plate Ventilation Space on Cell Growth in vitro" Zoological Science. 8. 197-199 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] MIEKO KARIYA,HIDEO NAMIKI: "The Effect of Culture Plate Ventilation Space on Cell Growth in vitro" ZOOLOGICAL SCIENCE. 8. (1991)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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