Regioselective Synthesis of Sugar-chain Unit Involved in Glycoconjugate by Glycosidase
| Project/Area Number |
02806017
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
応用生物化学・栄養化学
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| Research Institution | Shizuoka University |
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Principal Investigator |
USUI Taichi Shizuoka University, Faculty of Agriculture : Professor, 農学部, 教授 (50111802)
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| Project Period (FY) |
1990 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1991: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1990: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Glycoconjugate / Transglycosylation / Regioselective Synthesis / Glycosidase / N-acetyllactosamine / Regioselective Synthesis / Nーacetyllactosamine / 複合糖質 / 糖鎖認識単位 / 酵素合成 |
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| Research Abstract |
N-Acetyllactosamine 1(Gal beta1-4GlcNAe)is known as a representative disaccharide which is itself important as a core component in oligosaccharide moieties of glycoproteins and glycolipids. Owing to the growing need for substantial amount of 1 as a biomaterial, we have developed a practical route for the synthetic of 1 from lactose and Nacetylglucosamine(GlcNAc), utilizing the transglycosylation reaction through beta-D-galactosidase from Bacillus circulans. Furthermore, utilization of the transglycosylation by B. circulans beta-D-galactosidase led to the synthesis of beta-(1-4)linked disaccharides and trisaccharides containing a 2-acetamido-2-deoxy-D-hexose. The derivative s of GlcNAe and GalNAc were used as acceptors for the preparations. The enzyme reaction was efficient enough to allow us to perform the one pot preparation of beta-(1-4)-linked galactosyl-oligosaccharide from lactose. With the enzyme, D-galacosyl transfer occurred preferentially at the C-4 position of GlcNAc and of GalNAc, respectively, of the configuration of the hydroxyl group.
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Report
(3 results)
Research Products
(10 results)
