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Investigation into the Development of Immunobinding Test Kit for Detecting Avian Mycoplasma Antibodies

Research Project

Project/Area Number 02806057
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Applied veterinary science
Research InstitutionMiyazaki University

Principal Investigator

SHIMIZU Takamasa  Miyazaki Uni., Fac. of Agriculture, Professor, 農学部, 教授 (10040825)

Co-Investigator(Kenkyū-buntansha) NAGATOMO Hiroshi  Miyazaki Univ. Fac. of Agriculture, Assistant professor, 農学部, 助教授 (10041063)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1991: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1990: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywordsimmunobinding / Serological test / Avian mycoplasmas / Diagnostic kit / Antibody / 血清学的検査 / マイコプラズマ / イムノバインド反応 / 診断キット / 血清反応
Research Abstract

An attempt was made to develop an immunobinding(IB)test kit for detecting serum antibodies against Mycoplasma gallisepticum(MG)and V. synoviae(MS)in lieu of routine agglutination antigen which frequently gives rise to unspecific reaction. Commercially available plastic plates with 10 wells and two sorts of nitrocellulose membranes were compared as the MG and MS antigens. It was revealed that the plastic plate were more convenient to handle, although developed reaction spots disappeared within 2 weeks. in contrast, positive spots appered on nitrocellulose strips had been kept for more than 2 years without discolarization. The IB reaction was more sensitive than the HI test. The former test could detect MG or MS antibodies in all of 10 SPF chickens one week postinoculation with either of the two mycoplasmas, whereas two weeks were required for for the latter test to detect antibodies in all inoculated birds. The test results have indicated that, if a test kit including immobilized MG and MS antigens and test reagents are commercially available, the IB test can be routinely carried out on chicken rtaising farms.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Shimizu,T.,et al.: "Detection of serum antibodies against Mycoplasma gallisepticum and Mycoplasma synoviae by a dot-immunobinding technique." Japanese Journal of Veterinary Science. 52. 191-197 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Shimizu,T.and Nagatomo,H.: "Simultaneous detection of serum antibodies against Mycoplasma gallisepticum and M.synoviae by a dot-immunobinding on a durapore paper." Zentralblatt fur Bakteriologie. Suppl.880-882 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Shimizu, T., et al: "Detection of serum antibodies against Mycoplasma gallisepticum and Mycoplasma synoviae by a dot-immunobinding technique." Jap. J. Vet. Sci.52(2). 191-197 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Shimizu, T. and Nagatomo, H: "Simultaneous detection of serum antibodies against Mycoplasma and M. synoviae bu a dot-immunobinding on a durapore paper." Zbt. Bakt., Suppl. 20. 880-882 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Shimizu,T.,et al.: "Detection of serum antibodies against Mycoplasma gallisepticum and Mycoplasma synoviae by a dotーimmunobinding technique." Japanese Journal of Veterinary Science. 52. 191-197 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] Shimizu,T.and Nagatomo,H.: "Simultaneous detection of serum antibodies against Mycoplasma gallisepticum and M.synoviae by a dotーimmunobinding on a durapore paper." Zentralblatt fur Bakteriologie. 880-882 (1990)

    • Related Report
      1991 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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