| Project/Area Number |
02807035
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Tokyo Metropolitan Institute of Gerontology |
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Principal Investigator |
YAMAMOTO Mari Tokyo Metro. Inst. Geront., Dept. Cell Biol., Assistant, 細胞生物学部門, 助手 (90211648)
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| Co-Investigator(Kenkyū-buntansha) |
OOYAMA T. Tokyo. Metro. Inst. Geront. ., Dept. Cell Biol., Scientist, 生体情報部門, 研究員 (90183243)
YAMAMOTO K. Tokyo. Metro. Inst. Geront., Dept. Cell Biol., Chief Scientist, 細胞生物学部門, 主任研究員 (90073022)
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| Project Period (FY) |
1990 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1991: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1990: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Vascular endothelial cells / beta-APP / Localization and distribution / Deposition of beta-APP on basement membrane / Cellular aging |
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| Research Abstract |
By the immunocytochemical, immunochemical, and immunoelectron microscopic method using monoclonal antibodies against predictive synthetic beta-protein with the sequence of pesidure 597-620, the distribution and localization of beta-ainyloid protein precursor(beta-APP)in cultured vascular cells was investigated. beta-APP immunoreactivity was demonstrated in the plasma membranes of the cultured vascular cells as well as in the cytoplasma of the cells. Cytoplasmic beta-APP immunoreactivity located in the proximity of the cell nucleus and looked as microbody structure with various size. beta-APP immunoreactivity was also demonstrated on the surface of the basement membrane formed in the plastic dish during the culture of the cells. These findings suggest that the abnormal production or secretion of beta-APP in the vascular wall cells could be a cause of the formation of amyloid angiopathy in the Alzheimer's disease.
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