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Study on a fraction with relative radioresistency from murine pluripotent hemopoietic stem cells.

Research Project

Project/Area Number 02807042
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionYokohama City University, Sohool of Medicine

Principal Investigator

INOUE Tohru  Yokohama City Univ. School of Med. Assoc. Professer, 医学部・病理学第一講座, 助教授 (50100110)

Co-Investigator(Kenkyū-buntansha) 平林 容子  東京大学, 医科学研究所・分子生物学研究部, 学術振興会特別研究員
SASAKI Hideki  Yokohama City Univ. Med. Assistant Professer., 医学部・小児科学講座, 講師 (50106316)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1991: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1990: ¥900,000 (Direct Cost: ¥900,000)
KeywordsHemopoietic stem cells / CFU-S (colony forming Unit S) / spleen colonies. / Radiation sensitivity
Research Abstract

According to our suty on tridated thymidine cytocide, a colony forming unit in spleen (CFLJ-S) can. be divided into two major fractions ; i. e. stem cells possessing less pluripotency in differentiation with a Idnetics largely in S phase (the progenitor cells producing early appearing colonies), and the other ones possessing quiescent nature without cell cycle (the progenitor cells producing late appearing colonies). While former fraction reacts at larger extent to a variety of hemopoietic stimuli, latter does but relatively at a smaller extent. The heterogeneity of the CFU-S, as seen above, suggestes a heterogeneous radiation sensifivity in 6ach sub-fraction, however, none has been actually demonstrated pertaining to this heterogeneous radiation sensitivity. Bone marrow cells were irradiated with 400 cOy through 600 eGy, in vitro, and then been injected with lethally irradiated mice to form spleen colonies. There was a fraction found after these relatively larger dose of radiation at … More larger extent than numbers estimated by a survival curve fitting to a single-exponential model ; namely, the steep regular survival curve (D_0=90 cGy) changed into a flatter bne (D_0=270 cGy) after 300 through 400 cGy, probably because of a small radiosensitive subfraction, ca. 3-5%, mixed. with the regular CFLJ-S. This was confirmed by a variety of different murine stanns in our research project. Although irradiation was given at in vitro, oxygen effects must be minimum or none, and actually, no effects might have been involved in a mechanism of these flatter part of the survival curve, since total radiation time took for each fraction was ca. 4 min. and 14 sec. and or less. During these studies, we incidentally found another important finding in the Scid mice pertaining to the radioresistant fraction of the CFLJ-S. Originally, somatic cells in Scid mutaidon have been found to be very mioresistant because of a functional defect of repair for double strand breaks. We found, under the surveillance of D_0 in the Scid mice, again, a relatively radioresistant fraction sharply defined from the regular CFU-S. This phenomenon would deal with further study of this research program. Less

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (41 results)

All Other

All Publications (41 results)

  • [Publications] Hirabayashi Y,Inoue T,Mitsui H,Taniguchi T,Nishikawa S,Nakauchi et al: "Primitive hemopoietic stem cells-an obligatory stage in maturation before spleen colony formation." Proc National Acad Sci,U.S.A.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hirabayashi Y,Inoue T,Suda Y,Aizawa S,Ikawa Y,Kanisawa M.: "Higher incidence of hemopoietic neiplasms in lethally irradiated mice repopulated with bone marrow cells carrying the human c-myc oncogene." Exptl Hematol. 20. 167-172 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nishimura M,Kaku K,Azuno Y,Okafuji K,Etoh Y,Shiozaki H,Inoue T,Kaneko T.: "Effect of erythroid differentiation factor on megakaryocytic differentiation of L8057,a murine megakaryoblastic leukemia cell line." BHiochem Biophys Res Comm. 181(3). 1942-1047 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Tanaka T,Suda T,Suda J,Inoue T,Hirabayashi Y,Hirai H,Takaku F,Miura Y: "Stimulatory effects of granulocyte colony-stimulating factor on colony-forming units-spleen (CFU-S) differentiaton and pre-CFU-s proliferation in mice." Blood. 77. 2597-2602 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Fujimoto K,Kawakita M,Kato K,Yonemura Y,Matsuda T,Matsuzaki et al: "Purification of megakaryocyte differentiation activity from a fibrous histiocytoma cell line:N-terminal swquence homology to Activin A." Riochem Biophys Res Comm.174. 1163-1168 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sasaki H,Kajigaya Y,Hirabayashi Y,Funabiki T,Inoue T,Yokota T,et al.: "Establishment and characterization of a murine megakaryoblastic leukemia cell line growing in protein-free culture (L8057Y5)." Leukemia. 5. 408-415 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Iwasawa T,Hirabayashi Y,Kubota N,Inoue T,Kakei M,Matsui K.: "Hyperthermic purging in vitro of murine leukemia cells (MK-8057);Surviving fractions fo normal and leukemic stem cells and the longterm survival of mice injected with the post-hyperthermic leukemia cells." Exptl Hematol. 19. 332-337 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hirabayashi Y,Inoue T,Yoshida K,Sasaki H,Kubo S,Kanisawa M et al.: "Murine acute leukemia cell line with a single megakaryocytic differentiation (MK8057),induced by whole body irradiation in C3H.He mice:Cytological properties and kinetics of its leukemic stem cells." Intnl J Cell Cloning. 9. 24-42 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hirabayashi Y,Inoue T,Yoshida K,Inayama Y,Kanisawa M.: "The detection of normal hidden stem cells during the development of leukemia:Assays with PGK isozyme." Exptl Hematol. 18. 289-296 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 平林 容子,井上 達: "胚幹細胞や遺伝子導入マウスによる胎生期造血研究" 造血因子3(1):32ー39,1992. 3. 32-39 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 平林 容子,井上 達: "特集「造血微小環境」:加齢による造血微小環境の変化" 血液・腫瘍科. 23. 193-201 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 井上 達,平林 容子他: "造血幹細胞の病理ー多能性幹細胞をめぐる新知見と問題点ー" 横浜医学. 4. 153-176 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hirabayashi Y, Inoue T, Suda Y, Aizawa S, Ikawa Y, Kanisawa M.: "Higher indicence of hemopoietic neoplasms in lethally irradiated mice repopoulated with bone marrow cells carrying the human c-myc oncogene." Exptl Hematol. 20. 167-172 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nishimura M, Kaku K, Azuno Y, Okafuji K, Etoh Y, Shiozaki H, Inoue T, Kaneko T. et al.: "Effect of erythroid differenciation factor on megakaryocytic differentiation of L-8057, a murine megakaryoblastic leukemia cell line." Biochem. Biophys. Res. Comm. 181(3). 1842-1947 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Tanaka T, Suda T, Suda J, Inoue T, Hirabayashi Y, Hirai H, Takaku F, Miura Y.: "Stimulatory effects of granulocyte colony-stimulating factor on colony-forming units spleen (CFU-S) differentiation and pre-CFU-s proliferation in mice." Blood. 77. 2597-2602 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Fujimoto K, Kawakita M, Kato K, Yonemura Y, Masuda T, Matsuzatki et al.: "Purification of megakaryocyte differentiation activity from a fibrous histiocytoma cell line : N-terminal sequence homology to Activin A." Boichem. riophys. Res. Comm.174. 1163-1168 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sasaki H, Kajigaya Y, Hirabayashi Y, Funabiki T, Inoue T, Yokota T, et al.: "Establishment and characterization of a murine megakaryoblastic leukemia cell line growing in protein-free culture (L8057Y5)." Leukemia. 5. 408-415 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Iwasawa T, Hirabayashi Y, Kubota N, Inoue T, Kakei M, Matsui K.: "Hyperthermic purging in vitro of murine leukemia cells (MK-8057) : Surviving fractions of normal and leukemic stem cells and the long-term survival of mice injected with the post-hyperthermic leukemia cells." Exptl Hematol. 19. 332-337 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hirabayashi Y, Inoue T, Yoshida K, Sasaki H, Kubo S, Kanisawa M. et al.: "Murine acute leukemia cell line with a single megakaryocytic differentiation (MK8057), induced by whole body irradiation in C3H/He mice : Cytological properties and Kinetics of its leukemic stem cells." Intnl. J Cell Cloning. 9. 24-42 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hirabayashi Y, Inoue T, Yoshida K, Inayama Y, Kanisawa M.: "The detection of normal hidden stem cells during the development of leukemia : Assays with PGK isozyme." Exptl Hematol. 18. 289-296 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hirabayashi Y, and Inoue T.: "Embryonal hemopoietic study using embryonic stem cells and transgenic mice. (in Japanese)" Hemopoietic Factor. 3(1). 32-39 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hirabayashi Y, and Inoue T.: "Hemopoietic microenvironment during ageing. (in Japanese)" Hematology & Oncology. 23(3). 193-201 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Inoue T, Hirabayashi Y, Iwasawa T, Sasaki H, Umeda M.: "Pathology of the hemopoietic stem cells. (in Japanese)" Yokohama Medical J.42. 153-176 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hirabayashi Y,Inoue T,Mitsui H,Taniguchi T,Nishikawa S,Nakauchi et al: "Primitive hemopoietic stem cellsーan obligatory stage in maturation before spleen colony formation." Proc National Acad SCi,U.S.A.

    • Related Report
      1991 Annual Research Report
  • [Publications] Hirabayashi Y,Inoue T,Suda Y,Aizawa S,Ikawa Y,Kanisawa M.: "Higher incidence of hemopoietic neiplasms in lethally irradiated mice repopulated with bone marrow cells carrying the human cーmyc oncogene." Exptl Hematol. 20. 167-172 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Nishimura M,kaku K,Azuno Y,Okafuji K,Etoh Y,Shiozaki,H,Inoue T,Kaneko T.: "Effect of erythroid differentiation factor on megakaryocytic differentiation of L8057,a murine megakaryoblastic leukemia cell line." Biochem Biophys Res Comm. 181(3). 1942-1047 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Tanaka T,Suda T,Suda J,Inoue T,Hirabayashi Y,Hirai H,Takaku F,Miura Y: "Stimulatory effects of granulocyte colonyーstmulating factor on colonyーforming unitsーspleen (CFUーS) differentiaton and preーCFUーs proliferation in mice." Blood. 77. 2597-2602 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Fujimoto K,Kawakita M,Kato K,Yonemura Y,Matsuda T,Matsuzaki et al: "Purification of megakaryocyte differentiation activity from a fibrous histiocytoma cell line: Nーterminal swquence homology to Activin A." Riochem Biophys Res Comm.174. 1163-1168 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Sasaki H,kajigaya Y,Hirabayashi Y,Funabiki T,Inoue T,Yokota T,et al.: "Establishment and characterization of a murine megakaryoblastic leukemia cell line growing in proteinーfree culture (L8057Y5)." Leukemia. 5. 408-415 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Iwasawa T,Hirabayashi Y,Kubota N,Inoue T,Kakei M,Matsui K.: "Hyperthermic purging in vitro of murine leukemia cells(MKー8057); Surviving fractions of normal and leukemic stem cells and the longterm survival of mice injected with the postーhyperthermic leukemia cells." Exptl Hematol. 19. 332-337 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hirabayashi Y,Inoue T,Yoshida K,Sasaki H,Kubo S,Kanisawa M et al.: "Murine acute leukemia cell line with a single megakaryocytic differentiation(MK8057),induced by whole body irradiation in C3H.He mice: Cytological properties and kinetics of its leukemic stem cells." Intnl J Cell Cloning. 9. 24-42 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hirabayashi Y,Inoue T,Yoshida K,Inayama Y,Kanisawa M.: "The detection of normal hidden stem cells during the development of leukemia:Assays with PGK isozyme." Exptl Hematol. 18. 289-296 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 平林 容子,井上 達: "胚幹細胞や遺伝子導入マウスによる胎生期造血研究" 造血因子3(1):32ー39,1992. 3. 32-39 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 平林 容子,井上 達: "特集「造血微小環境」:加齢による造血微小環境の変化" 血液・腫瘍科. 23. 193-201 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 井上 達,平林 容子 他: "造血幹細胞の病理ー多能性幹細胞をめぐる新知見と問題点ー" 横浜医学. 4. 153-176 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Iwasawa T,Hirabayashi Y,Kubota N,Inoue T,Kakehi M,Matsui K:"Hyperthermic purging in vitro of murine leukemia cells (MKー8057):Surviving fractions fo normal and leukemic stem cells and the longterm survival of mice injected with the postーhyperthermic leuk cell." Experimental Hematology. 19. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Sasaki H,Kajigaya Y,Hirabayashi Y,Funabiki T,Inoue T,Yokota T,Ikuta K,Arai K,Matsuyama S.: "Establishment and characterization of a murine megakaryoblastic cell line growing in proteinーfree culture (L8057Y5)." Leukemia. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hirabayashi Y,Inoue T,Yoshida K,Sasaki H,Kubo S,Kanisawa M,and Seki M.: "Murine acute leukemia cell line with megakaryocytic differentiation (MKー8057) induced by wholeーbody irradiation in C3H/He mice: Cytological properties and kinetics of its leukemic stem cells." International Journal of Cell Cloning. 9. 24-42 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hirabayashi Y,Inoue T,Yoshida K,Inayama Y,Kanisawa M:"The detection of normal hidden stem cells during the development of leukemia:Assays with PGK isozyme." Experimental Hematology. 18. 7-10 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hirabayashi Y,Inoue T,Suda Y,Aizawa S,Ikawa Y,Kanisawa M:"Higher incidence of hemopoietic neoplasms in lethally irradiated mice repopulated with bone marrow cells carrying the human cーmyc oncogene." Experimental Hematology. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 井上 達、平林 容子、岩澤 夛恵、佐々木 秀樹、梅田 誠: "造血幹細胞の病理ー多能性幹細胞をめぐる新知見と問題点ー" 横浜直学. 41. (1991)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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