| Project/Area Number |
02807047
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
細菌学
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| Research Institution | Tokyo Women's Medical College |
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Principal Investigator |
UCHIYAMA Takehiko (1991) Tokyo Women's Medical College, School of Medicine, Professor, 医学部, 教授 (00050550)
内山 竹彦 東京女子医科大学, 医学部, 教授 (30072333)
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| Co-Investigator(Kenkyū-buntansha) |
KATO Hidehito Tokyo Women's Medical College, School of Medicine, Research Associate, 医学部, 助手
YAN Xiao-Jie Tokyo Women's Medical College, School of Medicine, Research Associate, 医学部, 助手 (80195627)
YAGI Junji Tokyo Women's Medical College, School of Medicine, Assistant Professor, 医学部, 講師 (70182300)
IMANISHI Ken'ichi Tokyo Women's Medical College, School of Medicine, Assistant Professor, 医学部, 講師 (20132920)
今西 健一 東京女子医科大学, 医学部, 講師 (80124527)
荒明 美奈子 東京女子医科大学, 医学部, 助手 (20075427)
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| Project Period (FY) |
1990 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1991: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1990: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | HUVEC / IFN-r / HLA class II / bacterial exotoxins / TSST-1 / SEs / HUVEC / ILー2 / ブドウ球菌外毒素 / MHCクラスII抗原 / T細胞受容体(Vβ) / 外毒素反応性Tハイブリド-マ / T細胞活性化 |
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| Research Abstract |
Human umbilical vascular endothelial cells (HUVEC) stimulated with recombinant human IFN-r were inveatigated for expression of HLA class II molecules, toxic shock syndrome toxin-1 (TSST-1) and staphylococcal enterotoxin A(SEA)-binding activity and accessory cell (AC) activity in T cell activation by TSST-1, SEA and related exotoxins. HUVEC stimulated with recombinant human IFN-r ranging from 10 to 3000 U/ml for 72 h express HLA class II molecules. Intensity of the expression was dependent on the concentration of IFN-r. HUVEC stimulated with 1000 U/ml of IFN-r for 72 h exhibited ^<125>I-TSST-1 and SEA-binding that was blocked by the unlabeled coresponding toxins and mAb to DR/DP. The activity was not blocked by paraformaldehyde fixation. The IFN-r-stimulated HUVEC exhibited AC activity in IL-2 production in human peripheral T cells stimulated with the several bacterial exotoxins. The activity was blocked by mAb to DR/DP. In view of the facts that TSST-1 and SEs bind directly to HLA class II molecules and activate human T cells in association with HLA class II molecules on AC, it is likely that the acquisition of the above activities in the toxin-induced T cell activation is mediated by the expression of HLA class II molecules. It seems likely that vascular endothelial cells play an important role in the development of pathological changes in patients suffering an acute and systemic illness TSS.
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