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Involvement of beta-carbolines in the symptoms of alcohol withdrawal

Research Project

Project/Area Number 02807066
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Legal medicine
Research InstitutionKobe University

Principal Investigator

ADACHI Junko  Kobe University School of Medicine assistant, 医学部, 助手 (40030887)

Co-Investigator(Kenkyū-buntansha) NAITO Takeaki  Kobe Women's College of Pharmacy professor, 薬学部, 教授 (00068339)
FUJIWARA Satoshi  Kobe University School of Medicine associate professor, 医学部, 助教授 (20173487)
TATSUNO Yoshitsugu  Kobe University School of Medicine professor, 医学部, 教授 (80030831)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1991: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1990: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordsbeta-carboline / alcoholism / alcoholic beverage / alcohol drinking / alcohol / acetaldehyde / βーカルボリン / アルコ-ル摂取 / アルコ-ル / トリプトファン / アルコ-ル症 / アルコ-ル飲料
Research Abstract

Tetrahydro-beta-carboline-3-carboxylic acid, a condensation reaction product of acetaldehyde, a metabolite of alcohol, with tryptophan can be considered a probable precursor of the benzodiazepine receptor antagonist, beta-carboline-3-carboxylate. beta-Carboline (norharman) and 1-methyl-beta-carboline (harman) have been prepared by the reaction of trytophan with some aldehydes under an oxidative condition. It is well known that these beta-carbolines inhabit monoamine oxidase and benzodiazepine receptor binding.
In the present study, a purification procedure, involving a chemically-bonded material followed by HPLC combined with fluorometric detection, was used for the quantitative determination of tetraphydro-beta-carbolines. An HPLC and mass spectrometry method was also developed for their identification. Norharman and harman in various alcoholic beverages could be detected, although the contents were very small. Moderate amounts of tetrahydro-beta-carboline-3-carboxylic acid were found in brewed alcoholic bevarage, however, distillled spilits contained low concentration.
Next, Urinary excretions of tetrahydro-beta-carbolines were measured in man after administration of ethanol (0.4g/kg). Blood ethanol and acetaldehyde levels were determined by gas chromatography. Ethanol intake caused increased excretion of tetrahydro-beta-carboline-3-carboxylic acid, though there was no significant correlation between blood acetaldehyde and urinary excretion of tetrahydro-beta-carbolines.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (5 results)

All Other

All Publications (5 results)

  • [Publications] 足立順子 他: "アルコール飲料及び食品中のβ-カルボリン(変異原物質)" アルコール代謝と肝. 10. 129-133 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 足立順子 他: "テトラヒドロ-β-カルボリンの生体内生成と飲酒の尿中排泄に及ぼす影響" アルコール代謝と肝. 11. 123-127 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Junko ADACHI et al: "Endogenous formation of tetrahydro-beta-carboline and effect of ethanol consumption on the urinary excretion" Alcohol Metabolism and the Liver. 11. 123-127 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 足立 順子,他: "テトラヒドローβーカルボリンの生体内生成と飲酒の尿中排泄に及ぼす影響" アルコ-ル代謝と肝. 11. 123-127 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 足立 順子,他: "アルコ-ル飲料及び食品中のβーカルボリン(変異原物質)" アルコ-ル代謝と肝. 10. (1991)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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