• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Synthesis of Mucus Glycoprotein in Ribosome of Rat Gastric Mucous Cell.

Research Project

Project/Area Number 02807073
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Gastroenterology
Research InstitutionKyoto University

Principal Investigator

TSUKADA Hideaki  Kyoto Univ., Faculty of Medicine, Assistant, 医学部, 助手 (30207336)

Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1991: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1990: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsGlycoprotein / Prostglandin / Glycosylation / Water-immersion Stress / Peptidyl-tRNA / PeptidylーtRNA / glycosylation / acylation / prostaglandin / ethanol / palmitic acid / galactosamine
Research Abstract

Using gastric mucous cells which are involved exclusively in the synthesis of secretory O-glycosidic glycoprotein (mucin), the relationships between protein core synthesis and its acylation with fatty acids, O-glycosylation with N-acetylgalactosamine were investigated Labeling of the cells with[^3H]-proline, [^3H]-palmitic acid, [^<14>C]-galactosamine followed by isolation of peptidy -tRNA and release of nascent peptides indicated that the nascent peptide were already glycosylated and acylated in the ribosome of the rat gastric mucous cell.
1. Prevention of alcohol-induced gastric mucosal injury by prostaglandin E-1 Effects of ethanol and prostaglandin on the glycoprotein synthesis in the gastric mucus cells have been evaluated. The rat gastric mucosal cells were subjected to a short-term tissue culture in the presence of ehtanol and prostaglandin El using[^3H]-proline, [^3H]-Palmitic acid, [^<14>C]-galactosamine. Low concentration of ehtanol (0.05-0.1M) stimulated the glycoprotein synt … More hesis, but higher concentration of ehtanol (0.5-1.5M) caused a marked reduction in the glycoprotein synthesis. Addition of prostaglandin resulted in the stabilization of the synthesis and secretion of the mucucs glycoprotein in the presence of ethanol.
2. lnfluence of water-immersion stress on synthesis of mucus glycoprotein in rat gastric mucosa.
Gastric raucous cells of rats subjected to water immersion stress were incubated with[^3H]-palmitic acid and[^<14>C]-N-acetylgalactosamine. The peptdyl-tRNA released by gastric polysomes was precipitated with cold ethanol and then the content was determined. A 70% reduction in the peptidyltRNA isolated was observed in the stress rats, as compared with in control rat. The peptide recoverd from the peptidyl-tRNA showed 30-50% less[3H]-Palmitic acid and[^<14>C]- N-acetylgalactosamine incorporation in the stressed rats than in normal control. It was thus suggested that translation, acylation and glycosylation of the peptides in the ribosomes of the gastric mucosa were remarkably affected by the stress. Less

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] 塚田 英昭・他: "ProstaglandinEー1のエタノ-ル胃粘液合成傷害に及ぼす影響" 日本消化器病学会雑誌. 87(10). 2342-2351 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 塚田 英昭・他: "ProstaglandinEー1の消化管粘膜に与える影響" 日本実験潰瘍研究会誌. 18. 80-84 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Tsukada H et al: "Prevention of Alcohol-induced Gastric Mucosal Injury by Porstaglandin E-1" Jap J Gastroenterolgy87. 10. 2342-2351 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Tsukada H et al: "Effect of Prostaglandin E-1 on the ion active transport in rat gastrointestinal mucosa." Experimental Ulcer 18. 80-84 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 塚田 英昭・他: "Prostaglandin Eー1のエタノ-ル胃粘液合成傷害に及ぼす影響" 日本消化器病学会雑誌. 87(10). 2342-2351 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 塚田 英昭・他: "Prostaglandin Eー1の消化管粘膜に与える影響" 日本実験潰瘍研究会誌. 18. 80-84 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 塚田 英昭・他: "Prostaglandin Eー1のエタノ-ル胃粘液合成傷害に及ぼす影響" 日本消化器病学会雑誌. 87(10). 2342-2351 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 塚田 英昭・他: "Prostaglandin Eー1の消化管粘膜に与える影響" 日本実験潰瘍研究会誌. 18. (1991)

    • Related Report
      1990 Annual Research Report

URL: 

Published: 1990-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi