| Project/Area Number |
02807098
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Pediatrics
|
|---|
| Research Institution | Kinki University |
|---|
Principal Investigator |
YOSHIOKA Kazuo Kinki University, Assistant professor of Pediatrics, 医学部, 講師 (60111035)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MURAKAMI Katsumi Kinki University, Medical Staff, 医学部, 助手 (50219888)
AKANO Norihisa Kinki University, Lecturer, 医学部, 講師 (10167223)
|
|---|
| Project Period (FY) |
1990 – 1991
|
| Project Status |
Completed (Fiscal Year 1991)
|
| Budget Amount *help |
¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1991: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1990: ¥1,100,000 (Direct Cost: ¥1,100,000)
|
|---|
| Keywords | IgA nephritis / IgA rheumatoid factor / Plasminogen activator / Plasminogen activator inhibitor / Transforming growth factor-b / In situ hybridization / insitu hybridization / ヤギIgG / フィブリン沈着 / 間質型コラ-ゲン / プロリン水酸化酵素 |
|---|
| Research Abstract |
1) Affinitiy to goat IgG of serum IgA from patients with IgA nephritis
ELISA showed that serum IgA from patients with IgA nephritis bound to goat IgG. Mean absorbance of ELISA in 32 IgA nephritis patients was segnificantly highir than that in normal human sera, but not difficant from that in other types of glomerulonephritis. IgA rheumaloid factor in plasma is not specific to IgA nephritis.
2) Tissue-type plasminogen activator and its inhibitor in human glomerulonephrtis 1)
We carried out an immunohistochemical study of tissue-type plasminogen activator (PA) and urokinase-type PA, and their inhibitors, PA inhibitor-1 and PA inhibitor-2, using renal biopsy specimens obtained from 86 patients with various forms of glomerulonephritis. The control were four normal renal tissue specimens.
On immunofluorescent observation, granular staining for tissue-type PA was found to be distributed along the glomerular capillary walls. The fluorescence was weak in the normal renal tissue and occasionally intense in the tissues of patients with IgA nephritis, minimal change nephrotic syndrome, and lupus nephritis. PA inhibitor-1 was abundant in the glomerular epithelial cells and scarce in the mesangial area and glomerular capillary lumens of the normal renal tissues. This was confirmed by immunoelectron microscopy using gold staining.
3) Transforming growth factor-beta protein and mRNA in glomeruli in normal and diseased human kidneys 2)
Expression of transforming growth factor-beta1 (TGF-beta1) in normal and diseased human kidneys was examined by immunohistochemical staining with two antibodies, and by in situ hybridization with an oligonucleotide probe.
The results indicate that mature TGF-beta and TGF-beta-LAP are localized in association with the matrix components of GBM or mesangium, and with immune deposit in human glomeruli. Expression of TGF-beta, mainly synthesized by mesangial cells, is enhanced in human glomerular diseases, and may contribute to the mesangial matrix increase.
|
