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Studies of mechanisms underlying tumor hypoxia

Research Project

Project/Area Number 02807105
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Radiation science
Research InstitutionNational Institute of Radiological Sciences

Principal Investigator

ANDO Koichi  NIRS, Div.Clin.Res., Section head, 臨床研究部, 室長 (00159526)

Co-Investigator(Kenkyū-buntansha) KOIKE Sachiko  NIRS, Div.Clin.Res., Senior researcher, 臨床研究部, 主任研究官 (50161831)
Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1992: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1991: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1990: ¥900,000 (Direct Cost: ¥900,000)
KeywordsHypoxic fraction / Intermediate hypoxia / Misonidazole / MRI / Perfusion mismatch / Blood flow / Blood volume / 細胞内グルタチオン / 蛍光色素 / 再酸素化 / 低酸素培養 / クロ-ン化細胞 / 血流遮断 / 新方法 / LーQモデル / 低酸素細胞 / 細胞亜集団 / クロ-ン株 / クロ-ン間相互作用
Research Abstract

Pathogenesis of tumor hypoxia was investigated for two murine fibrosarcomas transplanted into syngeneic C3H mice. Results obtained here were as follows. (1)R1137 fibrosarcomas with 7mm in diameter gave a single phase of cell survival curve with a Do of 1.3Gy when tumors were irradiated in vivo with Cs-137 gammarays. No hypoxic fraction was detected by lung colony assay. An i.p.-administration of Misonidazole failed to sensitize the tumor. Large-sized R1137 tumors showed again a single phase of cell survival curve, but gave a large Do value of 2.6Gy. Successful sensitization by misonidazole indicated that the large-sized R1137 tumors were under intermediate hypoxia. (2)Tumor blood flow and blood volume were measured by a method of Gd-DTPA enhanced nuclear magnetic resonance imaging(MRI). When dynamic change of T1 values for proton in tumors was analyzed by a compartment model, tumors with intermediate hypoxia showed a lower ratio of blood flow to blood volume than tumors with fractional hypoxia. (3)Tumors with intermediate hypoxia showed 30% perfusion mismatch in the blood vessels when Hoechst 33342 and DioC_7(3) were sequentially injected into mice bearing large R1137 tumors.

Report

(4 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] M.MUKAI,K.ANDO and S.KOIKE: "Effect of combined therapy,radiation and two local administration of OK-432,on a murine-fbrosarcoma(NFSa)" J.Jpn.Soc.Ther.Radiol.Oncol.4. 77-84 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K.ANDO,S.KOIKE and S.SATO: "Nonlinear survival curves for cells of solid tumors after large doses of fast neutrons and γrays" Radiat.Res.131. 157-161 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 安藤 興一,立崎 英夫: "放射線生物学からみた容積効果" 癌の臨床. 38. 1339-1343 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Y.OGISO,Y.YAMADA,K.ANDO,H.ISHIHARA and Y.SHIBATA: "Differential induction of prostaglandin E2-dependent and -independent immmune suppressor cells by tumor-derived GM-CSF and M-CSF" J.Leukocyte Biology. 53. 86-92 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] S.KOIKE,K.ANDO and H.IKEHIRA: "Relationship between radiobiological hypoxia and blood microcirculation in experimental tumors."

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.KOBAYASHI,K.KANDA,Y.UJENO,H.FUKUDA,K.ANDO,Y.MISHIMA,M.ICHIHASHI and J.HIRATSUKA: "Progress in neutron capture therapy for cancer" Plenum Press,New York, (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] H.FUKUDA,K.ANDO,C.KANDA,M.ICHIHASHI,Y.MISHIMA,J.HIRATSUKA,T.KOBAYASHI and K.KANDA: "Progress in neutron capture therapy for cancer" Plenum Press,New York, (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] M.Mukai, K.Ando and S.Koike: "Effect of combined therapy, radiation and two local administration of OK-432, on a murine-fibrosarcoma(NFSa)" J.Jpn.Soc.Ther.Radol.Oncol.4. 77-84 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K.Ando, S.Koike and S.Sato: "Nonlinear survival curves for cells of solid tumors after large doses of fast neutrons and gamma rays" Radiat.Res.131. 157-161 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K.Ando and H.Tatsuzaki: "Volume effect : From a Radio- biological Point of View" Jpn.J.Cacer Clin.38(12). 1339-1343 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Y.Ogiso, Y.Yamada, K.Ando, H.Ishihara and Y.Shibata: "Differential induction of prostaglandin E2-dependent and -independent immune suppressor cells by tumor-derived GM-CSF and M-CSF" J.Leukocyte Biol.(53). 86-92 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] S.Koike, K.Ando and H.Ikehira: Relationship between radiobiological hypoxia and blood microcirculation in experimental tumors,

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Kobayashi, K.Kanda, Y.Ujeno, K.Ando, Y.Mishima, M.Ichihashi and J.hiratsuka: Plenum Press, New York. Progress in neutron capture therapy for cacer, (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] H.Fukuda, K.Ando, C.Kanda, M.Ichihashi, Y.Mishima, J.Hiratsuka, T.Konayashi and K.Kanda: Plenum Press, New York. Progress in neutron capture therapy for cacer, (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] M.Mukai,K.Ando and S.Koike: "Effect of combined therapy, radiation and too local administration of OK-432, on a murine-fbrosarcoma(NFSa)" J.Jpn.Soc.Ther.Radiol.Oncol.4. 77-84 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] K.Ando, S.Koike and S.Sato: "Nonlinear survival curves for cells of solid tumors after doses of fast neutrons and γrays" Radiat.Res.131. 157-161 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 安藤 興一、立崎 英夫: "放射線生物学からみた容積効果" 癌の臨床. 38. 1339-1343 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Y.Ogiso, Y.Yamada, K.ando, H.Ichihashi and Y.Shibata: "Differential induction of prostaglandin E2-dependent and independent immmune suppressor cells by tumor-derived GM-CSF and M-CSF" J.Leukocyte Biology. 53. 86-92 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Kobayashi, K.Kanda, Y.Ujeno, H.Fukuda, K.Ando, Y.Mishima, M.Ichihashi and J.Hiratsuka: "Progress in neutron capture therapy for cancer" Plenum Press, New York, (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] H.Fukuda, K.Ando, C.Kanda, M.Ichihashi, Y.Mishima, J.Hiratsuka, T.Kobayashi and K.Kanda: "Progress in neutron capture therapy for cancer" Plenum Press, New York, (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 橋本 隆裕: "ラット脳の放射線壊死作成およびMRIによる評価" 日本磁気共鳴医学会雑誌. 11. 208-213 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Tatsuo Jibu: "Active components of intestinal bacteria for abdominal irradiation-induced inhibition of lung metastases." Clin.Exptl.Metastasis. 9. 529-549 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 安藤 興一: "放射線と抗癌剤の相互作用" 癌の臨床. 37. 1603-1607 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Koichi Ando: "Non-linear survival curves for solid tumor cells after large doses of fast neutrons and gamma rays." Radiation Research. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 安藤 興一: "基礎面からみた腫瘍治癒ー腫瘍幹細胞数の重要性についてー" 癌の臨床. 36. 2223-2226 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] S.Matsubara: "Chromosome aberration frequencies produced by a 70 MeV proton beam" Radiat.Res.123. 182-191 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] K.Ono: "Frequency of micronuclei in hepatocytes following X and fast neutron irradiations" Radiat.Res.123. 345-347 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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