| Project/Area Number |
02807154
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | IWATE MEDICAL UNIVERSITY |
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Principal Investigator |
IZUTSU Toshiko IWATE MEDICAL UNIVERSITY DEPARTMENT OF OBSTETRICS AND GYNECOLOGY ASSOCIATE PROFESSOR, 医学部, 助教授 (00103739)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUSHIMA Akimune IWATE MEDICAL UNIVERSITY DEPARTMENT OF OBSTETRICS AND GYNECOLOGY RESIDENT, 医学部, 助手 (20208937)
SATO Masayuki IWATE MEDICAL UNIVERSITY DEPARTMENT OF OBSTETRICS AND GYNECOLOGY RESIDENT, 医学部, 助手 (20183387)
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| Project Period (FY) |
1990 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1992: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1991: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1990: ¥500,000 (Direct Cost: ¥500,000)
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| Keywords | EGF / EGFR / C-myc / Ha-ras / Endometrial cancer / c-myc / Endometrial cencer / cーmyc / Haーras / Endomedrial Cancer / ER / PR |
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| Research Abstract |
I)Growth factor expression on endometrial cancer cells in vitro treated with steroid hormone Endometrial cancer cell line (Ishikawa, HEC-1) were treated various concentration of Estradiol-17B(E2) or Progesterone(P). GO+G1 phase cells were slightly increased after the treatment of E2 in Ishikawa cell line(IK). Epidermal growth factor (EGF) fluorescence intensity (EGF FI) of GO+G1 and S phase cells were slightly increased 48 hours after the addition of P in IK. Epidermal growth factor receptor(EGFR) fluorescent intensity (EGFR FI) of GO+G1 and S phase cells were slightly decreased 48 hours after the treatment of P in IK. S phase cells were markedly decreased after the treatment of P in HEC-1 cell line(HEC). EGF FI of GO+G1, S and G2+M phase cells were increased 24 hours after the treatment of P in HEC. EGFR FI of GO+G1, S and G2+M phase cells were increased 48 hours after the treatment of P in HEC.
II)Oncogene expression on endometrial cancer cells in vitro(IK) at various cell cycle phase High C-myc and Ha-ras fluorescence intensity was observed on G2+M phase cells in IK.
III)Oncogene expression under the control of cell cycle moduration C-myc oncogene fluorescence intensity in various cell cycle phase did not show stable tendency after the treatment of Thymidine(TDR) and Sodiumbutylate(SB) in IK. Ha-ras oncogene fluorescence intensity in various cell cycle phase was increased after the treatment of TDR and SB in IK.
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