Experimental Study of Aqueous Outflow Facility : Eye Perfusion Technique and Tissue Culture Methods
| Project/Area Number |
02807164
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Ophthalmology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
SUZUKI Ryo Yamaguchi University, School of Medicine, Associate Professor, 医学部, 助教授 (50163027)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUMOTO Tetsuo Yamaguchi University, School of Medicine, Professor, 医学部, 教授 (00040171)
YOSHINO Hideaki Yamaguchi University, University Hospital, Resident, 医学部附属病院, 医員
藤倉 義久 山口大学, 医学部, 講師 (10165368)
芳野 彦晃 山口大学, 医学部, 医員 (80182806)
中山 昌子 山口大学, 医学部, 助手 (10207954)
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| Project Period (FY) |
1990 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1991: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1990: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Eye perfusion technique / Tissue culture / Outflow facility / Anterior chamber perfusion / Posterior chamber perfusion / Glaucoma / 後房潅流 / 得内障 / 房水 / 毛様体 |
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| Research Abstract |
Outflow aqueous humor has been considered to pass through the trabecular meshwork(TM)down a pressure gradient without requiring pumping energy from the cells of the TM. However, trabecular outflow pathways may constitute more than merely a passive, structural filter for egress of aqueous humor from the eye.
TM cells(2nd or 3rd subculture) were placed cultured in sparse for six days and then exposed to media tested in both the presence or absence of either catalase and superoxide dismutase etc. The effects were examined by changes of outflow facility and cell shape. In a parallel experiment, cell shape change was observed with various cytoskeleton affecting drugs and by cell cytoskeleton (actin) and by gel electrophoresis.
To further understand the aqueous outflow mechanism, we also compared changes in the outflow facility with the tone and nerve-mediated contraction of ciliary muscle. NaF reduced the facility as much as 50%. lodoacetamide and iodoacetate both increased facility at 35 C. 2, 4 dinitrophenol, KCN and 2-deoxyglucose did not produce any effect. Theophylline and isobutylmethylxantnine increased outflow facility. However, neither epinephrine nor isoproterenol increased the, outflow facility, regardless of their concentrations. Epinephrine or isoproterenol(10^<-8>-10^<-6> M)did not relax the tone of bovine ciliary muscle, whereas theopliylline and IBMX remarkably relaxed the muscle which had been contracted by carbachol and also inhibited the nerve-mediated contraction.
These findings suggest that several substances in the aqueous humor may influence outflow facility. Morphological change(cell shape or cell attachment)of outflow tissues and the induced response of ciliary muscle may play a significant role on the outflow facility.
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Report
(3 results)
Research Products
(10 results)
