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Inhibition by salivary peptides, histatins on hydrogen peroxide generation from neutrophils.

Research Project

Project/Area Number 02807169
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionOkayama University

Principal Investigator

SUGIYAMA Katsumi  Okayama University Dental School, assistant professor, 歯学部, 助教授 (30032890)

Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1991: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1990: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsHuman Saliva / Histatins / Purification / neutrophil / H_2O_2 / Histamine release / Salivary conccentration / Diurnal changes / 活性酸素 / 肥満細胞
Research Abstract

1. Isolation and purification of histatins.
Heparin-affinity chromatography followed by reversedphase HPLC has been developed for the purification of human salivary histatins.
2. Inhibition by histatins on H_2O_2 generation from neutrophils.
Neutrophils obtained from guinea pig and human peripheral blood generated H_2O_2 accompanied by cellular adherence and spreading in the assence of stimulants. This spontaneous H_2O_2 production was dependent on temperature and Ca^<2+>, reaching the maximum in 30-60 min at 37゚C. Histatins inhibited this H_2O_2 generation dose-dependently. The IC_<50> value was 3, 5 and 32 muM for histatins 3, 5 and 1, respectively.
3. The essential moiety of histatins for histamine releasing activity.
The sequence of 9 residues of histatins, KRHHGYR(or K)RK was the structural requirements for the release of histamine from mast cells.
4. Characterization and quantitative determination of histatins.
A simple method for the assay of histatins in saliva by acid extraction followed by HPLC has been developed. Total histatin value was ranged from 8 to 56 muM with a mean value of 28 muM, and it was significant difference in age of donors for saliva. Diurnal changes of histatin secretion were not recognized. It was found that the histatins bound to the high molecular weight compondnts in saliva.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Yamanaka,Hiroyashu: "Rapid purification and characterization of histatins (histidine-rich poly peptides) from human whole saliva" Archs Oral Biol.35. 415-419 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 杉山 勝三: "ヒト唾液のヒスタチン(高ヒスチジン含有ペプチド)について" 歯科ジャ-ナル. 31. 466-476 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sugiyama,Katsumi: "High-performance liquid chromatographic separation and determination of histatins in human saliva" Archs Oral Biol.(1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yamanaka, Hiroyashu: "Cytolytic action of Vibrio vulnificus haemolysin on mast cells from rat peritoneal cavity" J. Med. Microbial.32. 39-43 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sugiyama, Katsumi: "Rapid purification and characterization of histatins (histidine-rich polypeptides) from human whole saliva." Archs Oral Biol.35. 415-419 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sugiyama, Katsumi: "Histatins (histidine-rich polypeptides) in human saliva" J. Dent. Med.31. 466-476 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Sugiyama, Katsumi: "High-performance liquid chromatographic separation and determination of histatins in human saliva" Archs Oral Biol.(1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Katsumi Sugiyama: "Highーperformance liquid chromatographic separation and determination of histatins in humam saliva." Archs Oral Biol.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yamanaka,Hiroyashu: "Cytolytic action of Vibrio vulnificus haemolysin on mast cells from rat peritoneal cavity" J.Med.Microbiol.32. 39-43 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Sugiyama,Katsumi: "Rapid purification and characterization of histatins (histidinerich polypeptides) from human whole saliva" Archs.oral Biol.35. 415-419 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 杉山 勝三: "ヒト唾液中のヒスタチン(高ヒスチジン含有ペプチド)について" 歯科ジャ-ナル. 31. 466-476 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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