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The Effects of Cytokines on Methabolism of Periodontal Fibroblasts

Research Project

Project/Area Number 02807172
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Conservative dentistry
Research InstitutionOkayama University

Principal Investigator

NOMURA Yoshio  Okayama University, Dental School, Associate Professor, 歯学部, 助教授 (50107075)

Co-Investigator(Kenkyū-buntansha) GOTO Hirouki  Okayama University, Dental School, Assistat, 歯学部, 助手 (90205609)
SHIMIZU Hideki  Okayama University, Dental School, Assistat, 歯学部, 助手 (70170983)
KINOSHITA Masahiko  Okayama University, Dental School, Assistant Professor, 歯学部附属病院, 講師 (80161537)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1991: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1990: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsFibroblast / Interleukin 1beta / Tumor necrosis factor alpha / Interleukin 6 / Interleukin 8 / Ttansforming growth factor / Prostaglandin E_2 / Cytokine network / 歯肉線維芽細胞 / DNA合成 / コラ-ゲン合成
Research Abstract

Fibroblasts are regarded as potential participants in the orchestration of inflammation via the production of inflammatory mediators. In addition, the metabolism of fibroblasts are effected various cytokines and growth factors.
The purpose of this study was to evaluate the effects of interleukin 1beta(IL-1beta)on metabolism of human gingival fibroblasts(HGF)and to evaluate the effects of various growth factors on metabolism of human periodontal ligament(HPL)cells.
The results were as follows ; l)IL-1beta inhibited the proliferation of HGF. 2)IL-1beta greatly stimulated PGE_2 production from HGF. 3)IL-1beta inhibited the collagen synthesis, while did not influence to no. ncollagenous protein synthesis. 4)Addition of indomethacin'reversed the inhibition of DNA and collagen synthesis caused by IL-1beta. 5)EGF, INS and TGF stimulated DNA synthesis in PDL cells. DNA synthesis was significantly enhanced by the combination of FGF and INS, FGF and TGF, EGF and INS, EGF and TGF. 6)INS and TGF stimulated collagen synthesis in PDL cells. 7)TGF was the only growth factor that enhanced the activity of ALPase in monolayer PDL cells. 8)With stimulating of either IL-1beta or TNFalpha, HGF expressed IL-6 and IL-8 RNA, secreted IL-6 and IL-8 in a dose dependent manner. 9)IL-6 secretion from HGF was partially supressed by PGE_2.10)Neutralization assay using a rabbit anti-recombina. nt human IL-8 antiserum inhibited the neutrophil chemotactic activity manifested by the cells to basal level. 11)HGF did not express IL-6 receptor MRNA. 12)In inflamed gingiva, HGF were stained with anti-IL-6 antibody.
These results suggested that HGF and PDL cells may play an important role to control the inflammatory process of periodontal disease.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] 滝川 雅之: "ヒト歯肉線維芽細胞の代謝に及ぼすインタ-ロイキン1βの影響" 日本歯科保存学雑誌. 35. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Shogo Takashiba: "Human girgival fibroblasts can produce neutrophil chemotactic factor (interleukin-8) in response to interleukin-1β and tumor recrosis factorα." Infection and Immunity.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takashi Katayama: "Effects of Growth Factors on Functions of Periodontal Ligament cells." Journal of Periodontal Research.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Masayuki TAKIGAWA, Yoshio NOMURA, Masahiko KINOSHITA, Hideki SHIMIZU, Hiroyuki GOTO, Hideo ARAI, Yutaka HIGUCHI, Norifumi WASHIO, Hidemi KURIHARA, Yoji MURAYAMA: "The Effects of Interleukin 1beta on Metabolism of Human Gingival Fibroblasts" Japan. J. Conserv. Dent.35(2). (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takashi KATAYAMA, Yoshio NOMURA, Masahiko KINOSHITA, Hidemi KURIHARA, Yoji MURAYAMA: Effects of Growth Factors on Functions of Periodontal Ligament Cells.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Shogo TAKASHIBA, Masayuki TAKIGAWA, Keiso TAKAHASHI, Fumio MYOKAI, Toshihiro CHIHARA, Hidemi KURIHARA, Yoshio NOMURA, Yoji MURAYAMA: "Interleukin-8 is a major neutrophil chemotactic factor derived from cultured human gingival fibroblasts stimulated with interleukin-1beta or tumor necrosis factor alpha"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 滝川 雅之: "ヒト歯周肉線維芽細胞の代謝に及ぼすインタ-ロイキン1βの影響" 日本歯科保存学雑誌. 35. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Shogo Takashiba: "Human gingival fibroblasts car produce reutrophil chemotactic factor (interleukinー8) in response to interleukinー1β and tumor necrosis factor α." Infection and Immunity.

    • Related Report
      1991 Annual Research Report
  • [Publications] Takashi Katayama: "Effects of Growth Factors on functions of Periodontal Ligament Cells." Journal of Periodontal Research.

    • Related Report
      1991 Annual Research Report
  • [Publications] 滝川 雅之: "歯肉線維芽細胞の機能発現に及ぼすILー1βの影響" 日本歯周病学会誌.

    • Related Report
      1990 Annual Research Report
  • [Publications] Shogo Takashiba: "Human gingival fibroblasts can produce neutrophil chemotactic factor (interleukinー8) in response to interleukinー1β and tumor necrosis factorα." Journal of Periodontal Research.

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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