Role of proteins related to blood coagulation and fibrinolysis in the prosecc of angiogenesis
| Project/Area Number |
02807210
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
医学一般
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| Research Institution | Ehime College of Health Science |
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Principal Investigator |
KON Kazunori Ehime College of Health scienc, Department of Clinical pathology, Associate Professor, 臨床検査学科, 助教授 (40093926)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIWARA Takashi Ehime University, School of Medicine, Associate Professor, 医学部, 助教授 (30036496)
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| Project Period (FY) |
1990 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1991: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1990: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Angiogenesis / Fibrin / Fibroblasts / Endothelial cells / Extracellular matrix / フィブリン(線維素) / 細胞間マトリックス / 線維素溶解 |
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| Research Abstract |
Angiogenesis during wound healing is associated with some events such as the increase in vasopermeability, the formation of extracellular fibrin matrix and the migration of fibroblasts. These phenomena are not well involved in the current mechanism of angiogenesis. This study was designed to relate the initial event occurring after injury to angiogenic response with the rabbit ear chamber method. The observation of growing circulation plexus showed characteristies of unstable blood circulation. Morphological study by a transmission electron microscopy showed that some blood flow pathways were surrounded by fibrin rich extracellular matrix and some tubular structures were composed of fibroblast like cells. When the formation of fibrin martix was inhibited with plasminogen activator, blood flow pathway collapsed. To define the role of fibroblasts in angiogenesis, cultured fibroblasts marked with india ink particles were added into the chamber. The fibroblasts were involved in the structure of the tubular structure. These data suggests that the mechanism of angiogenesis during wound healing is described as follows, i. e., blood circulation initially formed in the fibrin rich extracellular matrix then fibroblasts take place in the formation of blood vessels through differentiation of fibroblasts to endothelial cells.
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Report
(3 results)
Research Products
(7 results)
