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Studies on Induction of Salivary Cysteine Proteinase Inhibitor by Isoproterenol

Research Project

Project/Area Number 02807214
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 応用薬理学・医療系薬学
Research InstitutionNagoya University

Principal Investigator

NAITO Yukio  Nagoya City University Pharceutical Sciences Assistant Professor, 薬学部, 講師 (10076052)

Co-Investigator(Kenkyū-buntansha) MIZUTANI Takaharu  Nagoya City University Pharceutical Sciences Associate Professor, 薬学部, 助教授 (30080188)
SUZUKI Ikukatsu  Suzuka College of Technology Professor, 工業化学科, 教授 (90080209)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1991: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1990: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsRat cystatin S / Cystatin superfamily / Submandibular gland / Cysteine proteinase / Papain / Ficin / Trypsin / Protein induction / タンパク分解酵素阻害物質 / 細胞増殖 / 顎下腺唾液タンパク
Research Abstract

Rat cystain S(RSC), a cysteine proteinase inhibitor belonging to family 2 of the cystatin superfamily. (1)Papain(Ki : O. l nM)and ficin(Kl : 0.2 nM)were inhibited strongly, and bromelain and cathepsin C partially. The proteinases were inhibited by RSC competitively. However, trypsin, kalllkrein and pepsin did not inhibit by RSC. (2)When RSC was reduced and S-carboxymethylated, the proteinases inhibitory activity was lost completly. Complex formation between papain and RSC was 1 : 1 molar ratio. (3)The proteinase inhibitory activity of RSC was stable to extremes of pH 1-12 for I hour at 37 ゚C) and to heat treatment (100 ゚C for 1 hour). (4)Cysteine proteinases are important in the penetration of human tissue by microorganism. Anti-bacteria activity of RSC was tested to seventeen strains including of oral bacteriaby disc diffusion analysis. We found that RSC have anti-bacterial activity against Bacteroides gingivalis, an oral bacterium suspected of being a pathogen of periodontal disease(minimum effective dose : 75 mug/disc). (5)Treatment of rats with the beta-adrenergic-agonist, isoproterenol, produces hypertrophy and hyperplasia of submandibular glands and induces a large quantity of RSC. However, biological rule of the responses did not understand. When papain, a cysteine proteinase of plant(papaya), was administered into the oral cavity of rats of 8 weeks age, a dramatic increase in the level of RSC in enlarged submandibular glands. The magnitude of induction of RSC and enlargement of the glands were correlated with the dose of papain and duration of the treatment. We tested to specify on the respence in kind of proteinase. We found that ficin, a cysteine proteinase of plant(Ficus), and trypsin, a serine proteinase of pancreas, had same activity. We proposed that induction of the RSC in the submandibular glands by oral administration of the proteinases is a biological response which plays a role in preventing injury exgeneous proteinases.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] 内藤 幸雄: "ダイコクネズミCystatinsの安定性およびパパインとの相互作用" 医学と生物学. 121. 199-204 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 内藤 幸雄: "Induction of cystatin S in rat submandibular glands by papain." Comp.Biochem.Physiol.(1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yukio Naito: "Stability of rat cystatin S and interaction between it and papain." Medicine and Biology. 121(4). 199-204 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yukio Naito: "Induction of cystatin S in rat submandibular glands by papain." Comp. Biochem. Physiol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 内藤 幸雄: "Induction of cystatin S in rat submandibular glands by papain." Comp.Biochem.Physiol.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 内藤 幸雄: "ダイコクネズミとCystatin Sの安定性およびパパインとの相互作用" 医学と生物学. 121. 199-204 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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