| Project/Area Number |
02807227
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
内分泌・代謝学
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| Research Institution | Kagoshima University |
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Principal Investigator |
KAKEI Masafumi Kagoshima University, Medicine, Assistant Professor, 医学部, 助手 (90214270)
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Hiromitsu Kagoshima University, Medicine, Professor, 医学部, 教授 (80041292)
YADA Toshihiko Kagoshima University, Medicine, Associate Professor, 医学部, 助教授 (60166527)
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| Project Period (FY) |
1990 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1991: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1990: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Pancreatic B-cells / Ca channels / Insulin secretion / Ion channel / Pathch clamp / バッケクランプ |
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| Research Abstract |
Voltage-dependent Ca channels were studied with regard to glucose modulation in rat pancreatic B-cells. The single channel conductance was 28 pS at 55 mM Ba on the external side of the cell-attached patch membrane. In an effort to exclude the effects on the Ca channels of membrane potential changes due to closure of ATP-sensitive K channels by glucose metabolism, the resting membrane potential was chemically clamped at 0 mV by bathing the cell in the high K internal solution. The open-state probability of the Ca channels was increased 2-3 fold with an increase in glucose concentration for either 2.8 or 5.6 mM to 16.7 mM. Consistent effects of glucose on the channels were obtained in the presence of 1 mu M BAY K 8644. An open time histogram consisted of a single exponentia1 curve with a time constant of 0.2 ms and 0.29 ms at glucose concentration of 5.6 mM and 22.4 mM. respectively. Two time constants were required in the closed time histogram and they were 0.82 ms and 18.9 ms at 5.6 mM gucose. and 0.91 ms and 5.05 ms at 22.4 mM glucose. These results suggest that Ca-channel gating may be modulated by glucose metabolism.
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