• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Characteristics of the Ca channel from pancreatic B-cells and its modulation by glucose

Research Project

Project/Area Number 02807227
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 内分泌・代謝学
Research InstitutionKagoshima University

Principal Investigator

KAKEI Masafumi  Kagoshima University, Medicine, Assistant Professor, 医学部, 助手 (90214270)

Co-Investigator(Kenkyū-buntansha) TANAKA Hiromitsu  Kagoshima University, Medicine, Professor, 医学部, 教授 (80041292)
YADA Toshihiko  Kagoshima University, Medicine, Associate Professor, 医学部, 助教授 (60166527)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1991: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1990: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsPancreatic B-cells / Ca channels / Insulin secretion / Ion channel / Pathch clamp / バッケクランプ
Research Abstract

Voltage-dependent Ca channels were studied with regard to glucose modulation in rat pancreatic B-cells. The single channel conductance was 28 pS at 55 mM Ba on the external side of the cell-attached patch membrane. In an effort to exclude the effects on the Ca channels of membrane potential changes due to closure of ATP-sensitive K channels by glucose metabolism, the resting membrane potential was chemically clamped at 0 mV by bathing the cell in the high K internal solution. The open-state probability of the Ca channels was increased 2-3 fold with an increase in glucose concentration for either 2.8 or 5.6 mM to 16.7 mM. Consistent effects of glucose on the channels were obtained in the presence of 1 mu M BAY K 8644. An open time histogram consisted of a single exponentia1 curve with a time constant of 0.2 ms and 0.29 ms at glucose concentration of 5.6 mM and 22.4 mM. respectively. Two time constants were required in the closed time histogram and they were 0.82 ms and 18.9 ms at 5.6 mM gucose. and 0.91 ms and 5.05 ms at 22.4 mM glucose. These results suggest that Ca-channel gating may be modulated by glucose metabolism.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Yada.T.,Kakei,M.& Tanak,H: "Single pancreatic β-cells from morinal rats exhibit an initial decrease and subsequent increase in cytosolic Ca^<2+> in response to glucose" Cell Calcium. 13. 69-76 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 矢田 俊彦: "膵β細胞における分泌の生理学;グルコ-ス刺激ーインスリン分泌連関機構" 糖尿病記録号. 85-89 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 加計 正文他: "単離モルモット心室筋細胞のイオン電流に対するnicorandil,nitroprussideの作用機転" Therapeutic Research. 11. 16-21 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 矢田 俊彦・加計正文: "経口糖尿病薬のインスリン分泌刺激機構ー膵β細胞内Ca^<2+>シグナリングのチャンネル活動ー。" 実験医学. 8. 69-72 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 加計 正文: "膵β細胞のイオンチャンネルと刺激分泌連関機構" 医学のあゆみ. 156. 841-845 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 矢田 俊彦: "膵β細胞内のCaイオン濃度測定によるインスリン分泌機構の解析" 医学のあゆみ. 156. 846-850 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yada, T., Kakei, M. & Tanaka, H.: "Single pancreatic B-cells from normal rats exhibit an initial decrease and subsequent increase in cytosolic free Ca in response to glucose." Cell Calcium. 13. 69-76 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yada, T.: "Physiology of secretion in pancreatic B-cells : glucose stimulus-insulin secretion coupling." Tounyoubyou Kirokugo. 85-89 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kakei, M., Yoshinaga, M., Koyano, T. & Tanaka, H.: "Effects of nicorandil. nitroprusside on ionic currents of guinea-pig ventricular cells." Therapeutic Research. 11. 16-21 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yada, T. & Kakei, M.: "A stimulation of insulin secretion by antiduretic drugs : intracellular Ca signaling and ionic channel activity." Jikken Igaku. 8. 69-72 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kakei, M.: "Ionic channels and stimulus-secretion coupling in pancreatic B-cells." Igaku no ayumi. 156. 841-845 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yada, T.: "Analyses of insulin secretion by measurement of Ca ion concentration in pancreatic B-cells." Igaku no ayumi. 156. 846-850 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yada,T.,Kakei,M.&Tanak,H: "Single pancreatie βーcells from normal rats ehibit an initial decrease and subseguent increase in cytosolic Ca^<2+>in response to glucose" Cell Calcium. 13. 69-76 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 矢田 俊彦: "膵β細胞における分泌の生理学;グルコ-ス刺激ーインスリン分泌連関機構" 糖尿病記録号. 85-89 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 加計 正文 他: "単離モルモット心室筋細胞のイオン電流に対するnicorandil,nitroprussideの作用機転" Thcrapeutic Rescarch. 11. 16-21 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 矢田 俊彦・加計・正文: "経口糖尿病薬のインスリン分泌刺激機構ー膵β細胞内Ca^<2+>シグナリングとチャンネル活動ー" 実験医学. 8. 69-72 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 加計 正文: "膵β細胞のイオンチャンネルと刺激分泌連関機構" 医学のあゆみ. 156. 841-845 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 矢田 俊彦: "膵β細胞内Caイオン濃度測定によるインスリン分泌機構の解析" 医学のあゆみ. 156. 846-850 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 矢田 俊彦・加計 正文: "経口糖尿病薬のインスリン分泌刺激機構ー膵β細胞内Ca^<2+>シグナリングとイオンチャネル活動" 実験医学. 8(13). 69-72 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 加計 正文: "膵β細胞のイオンチャンネルと刺激分泌連関機構" 医学のあゆみ. 156. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 加計 正文: "Roles of glucoreーand ATPーdependent K channels in stimulusーsecretion coupling and actions of sulfonylureas in pancreatic βーcells" Jap.J.Pharmacal.52. 37-37 (1990)

    • Related Report
      1990 Annual Research Report

URL: 

Published: 1990-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi