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Role of Surface-Active Lipids in Fractal Morphogenesis.

Research Project

Project/Area Number 02808026
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 物質生物化学
Research InstitutionNiigata University

Principal Investigator

MATSUYAMA Tohey  Niigata University School of Medicine Dept. of Bacteriology, Associate Professor, 医学部, 助教授 (00047200)

Co-Investigator(Kenkyū-buntansha) NAKAGAWA Yoji  Niigata University General Education Dept. Associate Professor, 教養部, 助教授 (10018799)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1991: ¥100,000 (Direct Cost: ¥100,000)
Fiscal Year 1990: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsFractal / Morphogenesis / Surface-active lipids / Bacterial colony / Serratia marcescens / Bacterial lipids / 細菌集落 / 細菌脂質
Research Abstract

Surface-active exolipids produced by Serratia spp. were shown to be active in promotion of fractal colony morphogenesis. Structures of these novel lipids were determined. Serrawettin (form S. marcescens) W2 was a cyclodepsipeptide containing a fatty acid (3-hydroxydecanoic acid) and five amino acids. The peptide was proposed to be D-leucine (N-bonded to the carboylate of the fatty acid)-L-serine-L-threonine-D-phenylalanine-L-isoleucine (bonded to the 3-hydroxyl group). Rubiwettin (from S. rubidaea) R1 was proposed to be a mixture of 3-(3'-hydroxytetradecanoyloxy) decanoate, 3-(3'-hydoxyhexadecenoyloxy) decanoate and minor molecular isomers. The structure of rubiwettin RG1 was proposed to be beta-D-glucopyranosyl 3-(3'-hydroxytetradecanoyloxy) decanoate. As suggested by the diffusion-limited aggregation model (DLA) theoretically, fractal dimensions of bacterial colonies were shown to be 1.7 - 1.8 by the box-counting method. In the fractal colony growth, shielding and repulsion effects were also recognized. Thus, bacterial colony growth in a two-dimensional nutrient concentration field was shown to results in fractal pattern formation through diffusion-limited processes. Surface tension seems to exert profound effect on bacterial translocation on the agar surface. Biosufactant described by us may lighten such burden working at the growing colony periphery. Similar pattern formations were demonstrated by computer simulation of DLA model introducing a surface tension parameter.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Matsuyama, T.: "Surface-active novel glycolipid and linked 3-hydroxy fatty acids produced by Serratia rubidaea." J.Bacteriol.172. 3015-3022 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hara-Hotta,H.: "Mass spectrometry with soft ionization techniques for structual analysis of lipids in Serratia species." Anal.Chim.Acta. 247. 283-293 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Matsuyama,T.: "A novel extracellular cyclic lipopeptide which promotes flagelladependent and -independent spreading growth of Serratia marcescens." J.Bacteriol.174. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ohgiwari,M: "Morphological changes in growth phenomena of bacterial colony patterns." J.Phys.Soc.Jpn.61. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Matsuyama,T.: "Self-similar colony morphogenesis by gram-negative rods as the experimental model of fractal growth by a cell population." Appl.Environ.Microbiol.58. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 松山 東平: "医学・生物学におけるフラクタル" 朝倉書店, (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Matsushita,M.: "Growth patterns in physical science and biology." Plenum, (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Matsuyama, T., et al.: "Surface-active novel glycolipid and linked 3-hydroxy fatty acids produced by Serratia rubidaea." J. Bacteriol.172. 3015-3022 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hara-Hotta, H., et al.: "Mass spectrometry with soft ionization techniques for structual analysis of lipids in Serratia species." Anal. Chim. Acta. 247. 283-293 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Matsuyama, T., et al.: "A novel extracellular cyclic lipopeptide which promotes flagella-dependent and -independent spreading growth of Serratia marcescens." J. Bacteriol.174. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ohgiwari, M., et al.: "Morphological changes in growth phenomena of bacterial colony patterns" J. Phys. Soc. Jpn.61. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Matsuyama, T., et al.: "Self-similar colony morphogenesis by gram-negative rods as the experimental model of fractal growth by a cell population." Appl. Environ. Microbiol.58. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Matsushita, M., et al.: "Fractal growth and morphological changes in bacterial colony formation." Growth Patterns in Physical Science and Biology. Plenum. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Matsuyama,T.: "Surfaceーactive novel glycolipid and linked 3ーhydroxy fatty acids produced by Serratia rubidaea." J.Bacteriol.172. 3015-3022 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] HaraーHotta,H.: "Mass spectrometry with soft ionization techniques for structual analysis of lipids in Serratia species." Anal.Chim.Acta. 247. 283-293 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Matsuyama,T.: "A novel extracellular cyclic lipopeptide which promotes flagellaーdependent andーindependent spreading growth of Serratia marcescens." J.Bacteriol.174. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Ohgiwari,M: "Morphological changes in growth phenomena of bacterial colony patterns." J.Phys.Soc.Jpn.61. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Matsuyama,T.: "Selfーsimilar colony morphogenesis by gramーnegative rods as the experimental model of fractal growth by a cell population." Appl.Environ.Microbiol.58. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 松山 東平: "医学・生物学におけるフラクタル" 朝倉書店, (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Matsushita,M.: "Growth patterns in physical science and biology." Plenum, (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Matsuyama,T.: "Fractal spreading growth of Serratia marcescens which produces surface active exolipids." FEMS Microbiol.Lett.61. 243-246 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Matsuyama,T.: "Surfaceーactive novel glycolipid and linked 3ーhydroxy fatty acids produced by Serratia rubidaea." J.Bacteriol.172. 3015-3022 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Matsuyama,T.: "Fractal colony morphogenesis by gramーnegative rods under the influence of emvironmental factors." 発表予定.

    • Related Report
      1990 Annual Research Report
  • [Publications] Matsuyama,T.: "Cyclic aminolipids which promote flagellaーdependent swarming and flagellaーindependent fractal spreading growth of Serratia marcescens."

    • Related Report
      1990 Annual Research Report
  • [Publications] 松山 東平: "医学・生物学におけるフラクタル" 朝倉書店,

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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