Studies on Heterogeniety of General Transcription Factor TFIID

• Project/Area Number: 02808051
• Research Category: Grant-in-Aid for General Scientific Research (C)
• Allocation Type: Single-year Grants
• Research Field: 分子遺伝学・分子生理学
• Research Institution: Saitama Medical School (1991); Okazaki National Research Institutes (1990)
• Principal Investigator: TAMURA Taka-aki Saitama Medical School, Faculty of Medicine, Associate Professor, 医学部, 助教授 (30112692)
• Project Period (FY): 1990 – 1991
• Project Status: Completed (Fiscal Year 1991)
• Budget Amount: ¥1,600,000 (Direct Cost: ¥1,600,000); Fiscal Year 1991: ¥500,000 (Direct Cost: ¥500,000); Fiscal Year 1990: ¥1,100,000 (Direct Cost: ¥1,100,000)
• Keywords: Transcription factor / Transcriptional regulation

Research Abstract

The core promoter of the mouse myelin basic protein (MBP) gene from -36 to +12 was tissue-specifically (brain) transcribed. Biochemical studies demonstrated that TFIID fraction contains factors involved in such a specific transcription. Tnus. we hypothesized the existence of a tissuespecific TFIID. We isolated a cDNA clone for TFIID from the mouse brain, and compared its structure with the human TFIID. Few minor differences were observed only in the N-terminal portion of the mouse TFIID. The C-terminus that is required for the TFIID function was identical among two TFIIDs. Only a single TFIID gene was detected in mouse genome. Northern blot analysis demonstated that the TFIID transcript is expressedas the same size RNAs in mouse tissues. Mutation analysis within the MBP core promoter showed that the TATA-box is not essential but TATA-flanking sequences were important for tissue-specific transcription. From these results, there may be no tissue-specific TFIID. In these days, TFIID associating factors (TAF) have been identified by other groups. In our case, it is suggested that a tissue-specific TAF can be involved in the core promoter-mediated tissue-specific transcription.

Research Products

• [Publications] 田村 隆明,他: "Striking homology of the ‘Variable'Nーterminal as well as the ‘Conserved' domains of the mouse and human TATAーfactors(TFIID)" Nucleic Acids Research. 19. 3816-3865 (1991)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 田村 隆明,他: "Sequences involved in brainーspecific in vitro transcription from the core promoter of the mause myelin basic protein gene" Biochimica et Biophysica Acta. 1129. 83-86 (1991)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Taka-aki Tamura et al.: "Striking homology of the 'variable' N-terminal as well as the 'conserved' domains of the mouse and human TATA-factors (TFIID)." Nucleic Acids Research. 19. 3816-3865 (1991)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Taka-aki Tamura et al.: "Sequences in evolved in brain-specifc in vitro transcription from the core promoter of the mouse myelin basic protein gene." Biochim. et Biophys. Acta. 1129. 83-86 (1991)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] 田村 隆明,他: "Striking homology of the “Variable" Nーterminal as well as the “conserved" domains of the mouse and human TATAーfactors(TFIID)" Nucleic Acids Research. 19. 3816-3865 (1991)
• [Publications] 田村 隆明 他: "Sequences in volved in brainーspecific in vitro transcription from the core promoter of the mouse myelin basic protein gene" Biochimica et Biophysica Acta. 1129. 83-86 (1991)
• [Publications] 田村 隆明: "Core promter of the mouse myelin basic protein gene governs brainーspecific transcription in vitro" The EMBO Journal. 9. 3101-3108 (1990)