| Project/Area Number |
02808053
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
生物物性学
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
ITO Tadanao Kyoto Univetsity, Faculty of Science, Associate Professor, 理学部, 助教授 (90093187)
|
|---|
| Project Period (FY) |
1990 – 1991
|
| Project Status |
Completed (Fiscal Year 1991)
|
| Budget Amount *help |
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1991: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1990: ¥1,000,000 (Direct Cost: ¥1,000,000)
|
|---|
| Keywords | F-actin / cytoskeleton / cell volume regulation / ABP / gelsolin / water flow / osmotic stress / osmoelastic coupling / 細胞運動 / Fーアクチン / 溶積流 |
|---|
| Research Abstract |
we have studied the role of cytoskeleton composed of actin filaments(F-actin)in the cell volume regulation in vitro. We have used the Zimm-Myerson osmometer as a "model cell" and obtained following results.
(1)The F-actin in the 'model cell" retarded osmotically driven water flow across a semipermeable membrane and above a critical concentration at which F-actin predictably become immobilized(>3 mg/ml)water flow completely stopped.
(2)Actin-binding protein(ABP)promotes the perpendicular branching of F-actin, thereby serving as an efficient actin gelation protein. Gels of actin cross-linked by ABP completely stopped the osmotically driven-water flow at a low concentration of F-actin of 0.5 mg/ml
(3)Gelsolin severs F-actin in the presence of mm calcium, and solate the actin gel. When the gel was solated by calcium-activated gelsolin, osmotically driven water flow appeared.
These results suggest that the ABP-bases actin networks in the cell periphery could constitute a relative strong buffer against cell swelling or shrinkage by small fluctuation in osmotic pressures that constantly affect cell of mammalian organisms.
|
