Cooperation and information exchange with European countries.

• Project/Area Number: 03042012
• Research Category: Grant-in-Aid for international Scientific Research
• Allocation Type: Single-year Grants
• Section: Special Cancer Research
• Research Institution: University of Tokyo
• Principal Investigator: KUROKI Toshio Institute of Medical Science, University of Tokyo, Professor, 医科学研究所, 教授 (90006073)
• Co-Investigator(Kenkyū-buntansha): NIKAIDO Osamu Faculty of Pharmaceutical Sciences, Kanazawa University, 薬学部, 教授 (60019669) ; SEKI Shuji Institute of Cellular and Molecular Biology Okayama University Medical School, 医学部, 教授 (50032884) ; TANAKA Kiyoji Institute for Molecular and Cellular Biology Osaka University, 細胞生体工学センター, 教授 (80144450) ; HANAOKA Fumio The Institute of Physical and Chemical Research (RIKEN), 主任研究員 (50012670) ; MAKI Hisaji Institute of Molecular and Cellular Bioscience University of Tokyo, 分子細胞生物学研究所, 助教授 (20199649) ; 許 南浩 東京大学, 医科学研究所, 助教授 (70173573) ; ISHIKAWA Takatoshi Faculty of Medicine, University of Tokyo, 医学部, 教授 (30085633) ; SEKIGUCHI Mutsuo Medical Institute of Bioregulation, Kyushu University, 生体防御医学研究所, 教授 (00037342) ; HUH Nam-ho Institute of Medical Science, University of Tokyo ; 中島 元夫 東大, 応微物, 助教授 (90240738) ; 瀬戸 加大 愛知県がんセ, 研, 室長 (80154665) ; 長田 重一 大阪バイオサイエンス研, 部長 (70114428) ; 相沢 慎一 理研, つくば, 副主任研究員 (60073011) ; 堀井 明 癌研, 研究員 (40249983) ; 広橋 説雄 国立がンセ, 研, 部長 (70129625) ; 奥村 康 順天堂大, 医, 教授 (50009700) ; 勝木 元也 九大, 生医研, 教授 (20051732) ; 山岸 秀夫 京大, 理, 助教授 (90025429) ; 伊藤 嘉明 京大, ウイルス研, 教授 (80004612) ; 平野 俊夫 阪大, 医・バイオセ, 教授 (40136718) ; 菊谷 仁 阪大, 細胞工学セ, 助教授 (80161412) ; 谷口 維紹 阪大, 細胞工学セ, 教授 (50133616) ; 豊島 久真男 阪大, 微研, 教授 (90029760) ; 中村 祐輔 癌研究会癌研究所, 部長 (70217909) ; 吉田 廸弘 北海道大学, 理学部, 教授 (60001765) ; 大工原 恭 鹿児島大学, 歯学部, 教授 (40028733) ; 貝淵 弘三 神戸大学, 医学部, 助教授 (00169377) ; 御子柴 克彦 大阪大学, 蛋白質研究所, 教授 (30051840) ; 稲垣 昌樹 愛知県がんセンター, 研究所, 主任研究員 (30183007) ; 月田 承一郎 岡崎国立共同研究機構, 生理学研究所, 教授 (50155347) ; 西村 暹 国立がんセンター, 研究所, 部長 (20076970) ; 清水 孝男 東京大学, 医学部, 教授 (80127092)
• Project Period (FY): 1991 – 1993
• Project Status: Completed (Fiscal Year 1993)
• Budget Amount: ¥33,000,000 (Direct Cost: ¥33,000,000); Fiscal Year 1993: ¥11,000,000 (Direct Cost: ¥11,000,000); Fiscal Year 1992: ¥11,000,000 (Direct Cost: ¥11,000,000); Fiscal Year 1991: ¥11,000,000 (Direct Cost: ¥11,000,000)
• Keywords: International Exchange Program / French-Japanese Collaborative Program in Cancer Research / German-Japanese Collaborative Program in Cancer Research / Oncogenes / Tumor Suppressor Genes / Signal Transduction / DNA Repair / Cancer Metastasis / がん遣伝子 / がん抑制遣伝子

Research Abstract

In the fiscal year of 1993, the German-Japanese Workshop was held on 2-4 September 1993 at Institute of Cell Biology, Essen University, Essen, Germany. The workshop was entitled "Molecular and cellular aspects of carcinogenesis - DNA repair and its genes - ". From Japan following scientists participated by the support of this grant : T.Kuroki, T.Ishikawa, N.Huh, H.Maki (Univ. of Tokyo), F.Hanaoka (Riken), O.Nikaido (Kanazawa Univ.), K.Tanaka (Osaka Univ.), S.Seki (Okayama Univ.) and M.Sekiguchi (Kyushu Univ.). The French Japanese workshop was held on November 1-2, 1993 in Kanazawa under the title of "Tumor progression and molecular basis of oncogenesis", in which 12 French scientists and 11 Japanese scientists participated.
In addition to the above collaborative programs, following five scientists were sent to Europe to exchange information and materials : M.Seiki (Kanazawa Univ.), K.Koike (Cancer Institute), M.Shibuya (Univ. of Tokyo), T.Watanabe (Univ. of Tokyo) and Y.Nozawa (Gifu Univ.).

Research Products

• [Publications] Kuroki,T.: "Why are human cells resistant to malignant cell transformation in vitro?" Japan.J.Cancer Res.84. 1091-1100 (1993)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Sakumi K.: "Cloning and expression of cDNA for a human enzyme that hydrolyzes 8-Oxo-dGTP,a mutagenic substrate for DNA synthesis." J.Biol.Chem.268. 23524-23530 (1993)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Nakatsuru,Y.: "O^6-Methylguanine-DNA methyltransferase protects against nitros-amine-induced hepatocarcigenesis." Proc.Natl.Acad.Sci.USA. 90. 6468-6472 (1993)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Huh,N.: "Isolation and characterization of a novel hair follicle-specific gene,hacl-1." J.Inv.Dermatol.(in press). (1994)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Sugasawa,K.: "Cell-free repair of UV-damaged simian virus 40 chromosomes in human cell extracts." J.Biol.Chem.268. 9098-9104 (1993)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Satokata,I.: "Genomic characterization of the human DNA excision repair-controlling gene XPAC." Gene. 136. 345-348 (1993)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Seki,S.: "Activity gel and activity blotting methods for detecting DNA-modifying(repair)enzymes." J.Chromatogr.618. 147-166 (1993)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Matsunaga,T.: "Establishment and characterization of a monoclonaly,antibody recognizing the dewar isomers of(6-4)photoproducts." Photochem.Photobiol.57. 934-940 (1993)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kuroki, T.: "Why are human cells resistant to malignant cell transformation in vitro ?" Jpn. J. Cancer Res.84. 1091-1100 (1993)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Sakumi, K.: "Cloning and expression of cDNA for a human enzyme that hydrolyzes 8-Oxo-dGTP, a mutagenic substrate for DNA synthesis." J.Biol.Chem.268. 23524-23530 (1993)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nakatsuru, Y.: "O^6-Methylguanine-DNA methyl transference protects against nitrosamine-induced hepatocarcinogenesis." Proc.Natl.Acad.Sci.USA. 90. 6468-6472 (1993)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Huh, N.: "Isolation and characterization of a novel hair follicle-specific gene, hacl-1." J.Invest.Dermatol.(in press). (1994)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Sugasawa, K.: "Cell-free repair of UV-damaged simian virus 40 chromosomes in human cell extracts." J.Biol.Chem.268. 9098-9104 (1993)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Satokata, I.: "Genomic characterization of the human DNA excision repair-controlling gene XPAC." Gene. 136. 345-348 (1993)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Seki. S.: "Activity gel and activity blotting methods for detecting DNA-modifying (repair) enzymes." J.Chromatogr.618. 147-166 (1993)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Matsunaga, T.: "Establishment and characterization of a monoclonal antibody recognizing the dewar isomers of (6-4)photoproducts." Photochem.Photobiol.57. 934-940 (1993)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] OSADA,S: "A new member of the protein kinase C family,npkc ,predominatly expressed in skeletal muscle." Mol.Cell.Biol.12. 3930-3938 (1992)
• [Publications] OKA,T.: "Similarity,in molecular structure and function,between the plant toxin purothionin and the mammalian pore-forming proteins." Mol.Biol.Evol.9. 707-715 (1992)
• [Publications] SHIBUYA,H.: "IL-2 and EGF receptors stimulate the hematopoietic cell cycle via different signaling pathways:Demonstration of a novel role for c-myc." Cell. 70. 57-67 (1992)
• [Publications] YOKOTA,A: "Two forms of the low-affinity Fc receptor for IgE differentially mediate endocytosis and phagocytosis:Identification of the critical cytoplasmic domains." Proc.Natl.Acad.Sci.,USA. 89. 5030-5034 (1992)
• [Publications] KAISHO,T.: "Human bone marrow stromal cell lines from myeloma and rheumatoid arthritis which can support murine preB cell growth." J.Immunol.149. 4088-4095 (1992)
• [Publications] SATAKE,M.: "Differential expression of subspecies of polyomavirus and murine leukemia virus enhancer core binding protein,PEBP2, in various hematopoietic cells." Jpn.J.Cancer Res.83. 714-722 (1992)
• [Publications] KUBO,E.: "Novel TCR gene rearrangements and expression in rediation-induced thymic lymplomas." J.Radiat.Res.33. 227-242 (1992)
• [Publications] TAKAHASHI,K.: "CD4 and CD8 requlate interleukin 2 responses of T cells." Proc.Natl.Acad.Sci.USA. 89. 5557-5561 (1992)
• [Publications] SHIMOYAMA,Y.: "Cadherin dysfunction in a human cancer cell line:Possible involvement of loss of α-catenin expression in reduced cell-cell adhesiveness." Cancer Research. 52. 5770-5774 (1992)
• [Publications] WATANABE-FUKUNAGA,R.: "Lymphoproliferation disorder in mice explained by defects in Fas antigen that mediates apoptosis." Nature. 356. 314-317 (1992)
• [Publications] SAGA,Y.: "Mice develop normally without tenascin." Genes & Development. 6. 1821-1831 (1992)
• [Publications] ANDO,K.: "Chemically induced forestomach papillomas in transgenicmic mice carry mutant human c-Ha-ras transgenes." Cancer Research. 52. 978-982 (1992)
• [Publications] FABRA,A.: "Modulation of the invasive phenotype of human colon carcinoma cells by organ specific fibroblasts of nude mice." Differetiation. 52. 101-110 (1992)
• [Publications] SETO,M.: "Gene rearrangement o and overexpression of PRAD1 in lymphoid malignancy whth t(11:14)(q13;q32)translocation." Oncogene. 7. 1401-1406 (1992)
• [Publications] HORII,A.: "Frequent somatic mutations of hte APC gene in human pancreatic cancer." Cancer Research. 52. 6696-6698 (1992)
• [Publications] Honda,Z.: "Cloning by functional expression of plateletactivating factor receptor from quinea-pig lung." Nature. 349. 342-346 (1991)
• [Publications] Kuroki,T.: "Second Japanese-German workshop on molecular and cellular aspects of carcinogenesis." Int.J.Cancer. 346. 155-158 (1990)
• [Publications] Tchou,J.: "8-Oxoguanine (8-hydroxyguanine)DNA glycosylase and its substrate specificity." Proc.Natl.Acad.Sci.USA. 88. 4690-4694 (1991)
• [Publications] Nagafuchi,A.: "The 102kD cadherin-associated protein:Similarity to vinculin and phoposttranscriptional regulation of expression." Cell. 65. 849-857 (1991)
• [Publications] Nakagawa,T.: "The subtypes of the mouse sinositol 1,4,5-triphosphate receptor are expressed in a tissue-specific and developmentally specific manner." Proc.Natl.Acad.Sci.USA. 88. 6244-6248 (1991)
• [Publications] Kaibuchi,K.: "Molecular cloning of the cDNA for stimulatory GDP/GTP exchange protein for smg p21s (ras p21-like small GTP-binding proteins) and characterization of stimulatory GDP/GTP exchange protein." Mol.Cell.Biol.11. 2873-2880 (1991)
• [Publications] Tsubouchi,H.: "Levels of the human hepatocyte growth factor in serum of patients with various liver diseases determined by an enzyme-linked immunosorbent assay." Hepatology. 13. 1-5 (1991)
• [Publications] Ozawa,K.: "Assignment of the human CREB2 (CRE-BP) gene to 2q32." Genomics. 10. 1103-1104 (1991)
• [Publications] Kinzler,K.W.: "Identification of FAP locus genes from chromosome 5q21." Science. 253. 661-669 (1991)
• [Publications] Sakumi,K.: "Cloning and expression of cDNA for rat O^6-methylguanine-DNA methyltransferase." Nucleic Acids Res.19. 5597-5601 (1991)
• [Publications] Nishizawa,K.: "Specific localization of phospho-intermediate filament protein in the constricted area of dividing cells." J.Biol.Chem.266. 3074-3079 (1991)