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New developments of neurophysiology in marine mollusk

Research Project

Project/Area Number 03044152
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionOkazaki National Research Institutes

Principal Investigator

YAMAGISHI Shunichi  National Institute for Physiological Sciences, 生理学研究所, 教授 (70014032)

Co-Investigator(Kenkyū-buntansha) ケインズ R.D.  ケンブリッジ大学, 名誉教授
ホイットフィールド M  プリマウス海洋生物研究所, 所長
デントン E.  プリマウス海洋生物研究所, 前所長
ボーン Q  プリマウス海洋生物研究所, 名誉所員
ウィリアムソン R.  プリマウス海洋生物研究所, 上級研究員
ブラウン E.R.  プリマウス海洋生物研究所, 研究員
アボット N.J.  ロンドン大学, キングス校, 副教授
WATANABE Akira  Waseda University, Faculty of Human Science, 人間科学部, 教授 (30013791)
EBASHI Setsuro  National Institute for Physiological Sciences, 生理学研究所, 名誉教授 (10009863)
INOUE Isao  Tokushima University, 酵素科学研究センター, 助教授 (80001973)
TSUTSUI Izuo  National Institute for Physiological Sciences, 生理学研究所, 助手 (80202183)
KUKITA Fumio  National Institute for Physiological Sciences, 生理学研究所, 助手 (40113427)
KEYNES Richard D.  Cambridge University
DENTON Eric  Plymouth Marine Biological Association Laboratory
WILLIAMSON Roddy  Plymouth Marine Biological Association Laboratory
BROWN Euan  Plymouth Marine Biological Association Laboratory
ABBOTT Joan N.  University London, King's College
WHITFIELD Michael  Plymouth Marine Biological Association Laboratory
メッセンジャー J B  シェフィールド大学, 副教授
ウィリアムソン R  プリマスMBA研究所, 研究員
ブラウン E R  プリマスMBA研究所, 研究員
ケインズ R D  ケンブリッジ大学, 名誉教授
デントン E  プリマスMBA研究所, 前所長
アボット N J  ロンドン大学, キングス校, 副教授
ブラウン E.  ブリマスMBA研究所, 研究員
ボーン Q.  ブリマスMBA研究所, 研究員
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥20,000,000 (Direct Cost: ¥20,000,000)
Fiscal Year 1993: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1992: ¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1991: ¥8,000,000 (Direct Cost: ¥8,000,000)
KeywordsMarine mollusk / Schwann's cell / Nerve fiver / Ion channels / Potassium accumulation / Sensory cells / Excitation-contraction coupling / Molecular evolution / 神経生理学 / 頭足類 / 感覚生理学 / 動物愛護
Research Abstract

The Japan-U.K. scientific research program on "New development of neurophysiology in marine mollusk" started in 1991 under the support of the Ministry of Education, Science and Culture.
Through the visit to Plymouth MBA Laboratory from Japan(Kukita, Inoue, Tsutsui, Yamagishi and Ebashi) and the invitation to Japan(Abbott, Brown, Bone, Williamson), scientific collaboration on neurophysiology in marine mollusk has greatly progressed and has obtained the following results.
(1)Physiological and morphological studies on Schwann cell and its interaction with nerve fibers has progressed. We clarified the specific morphological feature, i.e. intercellular invagination of extracellular space by electronmicroscopy and cell to cell communication by the use of fluorescent dye microscopically. We succeeded to isolate a single Schwann cell form squid giant fiber. Then, using whole cell patch clamp technique, we found Ca^<2+> activated K^+-channel and non-selective cation channel in the Schwann cell.
(2)We recorded the pathway of nerve impulses evoked by light or electrical stimulation of sensory cells from the slice of octopus optic lobe with CCD camera.
(3)We confirmed that the DHP sensitive charge-movement is essential for the excitation-contraction(E-C) coupling of skeletal muscle cells. From the evolutional view point, the E-C coupling and the requirement of Ca^<2+> influx for muscle contraction has studied by measuring the Ca^<2+> current and charge movement on dissociated single muscle cell of 8 different species of sea animals(from Mollusk to vertebrate). We found that E-C coupling mechanism was evoked from the species of lower vertebrates

Report

(3 results)
  • 1993 Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report

Research Products

(32 results)

All Other

All Publications (32 results)

  • [Publications] Kukita,F.et al.: "Kinetic analysis of the denaturation process of sodium channels in squid giant axon by alcohls" J.Physiol.463. 523-543 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Inoue,I.et al.: "Comparative physiology of excitation-contraction coupling" Jap.J.Physiol.43. 275-278 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yamagishi,S.et al.: "The emergence of chloride conductance by intracellular ionic conditions in squid giant axons." Jpn J Physiol.43(s2). s171 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Inoue,I.et al.: "Evolution of skeletal muscle excitation-contraction coupling and the appearance of dihydropyridine-sensitive intramembrane charge movement" Proc.Roy Soc.Lond.B.(in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 山岸俊一: "イギリス生理学の伝統と現在" 医学のあゆみ. 166. 578-580 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] 山岸俊一: "海生生物を用いた神経・筋生理学の展開" 学術月報. (印刷中). (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Abbott,N.J.et al.: "Electrophysiology of squid Schwann cells.In:Cephalopod Neurobiology.(Ed.Abbott,N.J.,Williamson,R.& Maddok,L.)" Oxford University Press(in press), (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Inoue,I.et al.: "Resting and active K channels in the squid axon membrane.In:Cephalopod Neurobiology.(Ed.Abbott,N.J.,Williamson,R.and Maddok,L.)" Oxford University Press(in press), (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yamagishi,S.et al.: "The effect of internal Ca^<2+> and Mg^<2+> on ion channels in the squid giant axons.In:Cephalopod Neurobiology.(Ed.Abbott,N.,Williamson,R.and Maddock,L.M.)" Oxford University Press(in press), (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kishimoto,U.et al.: "Voltage dependence of the ouabain-sensitive current of the squid axon membrane.In:Cephalopod Neurobiology.(Ed.Abbott,N.J.,Williamson,R.and Maddok,L.)" Oxford University Press(in press), (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Pichon,Y.et al.: "Periaxonal K^+ accumulation and regulation in the squid.In:Cephalopod Neurobiology.(Ed.Abbott,N.J.,Williamson,R.and Maddok,L.)" Oxford University Press(in press), (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Inoue,I.et al.: "Evolution of skeletal muscle excitation-contraction coupling.In:“Towards molecular Buiphysics of Ion Channels"." Elsevier Science Inc.Amsterdam(in press), (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kukita,F.et al.: "Kinetic analysis of the denaturation process of sodium channels in squid giant axon by alcohols" J.Physiol.463. 523-543 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Inoue,I.et al.: "Comparative physiology of excitation-contraction coupling" Jap.J.Physiol.43. 275-278 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yamagishi,S.et al.: "The emergence of chloride conductance by intracellular ionic conditions in squid giant axons." Jpn J Physiol.43(s2). s171 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Inoue,I.et al.: "Evolution of skeletal muscle excitation-contraction coupling and the appearance of dihydropyridine-sensitive intramembrane charge movement" Proc.Roy Soc.Lond.B.(in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] ****: "*************." ******.166. 578-580 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] ****: "**********.*******" ****. (***). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Abbott,N.J.et al.: Oxford University Press(in press). Electrophysiology of squid Schwann cells. In : Cephalopod Neurobiology.(Ed.Abbott,N.J., Williamson,R. & Maddok,L.), (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Inoue,I.et al.: Oxford University Press(in press). Resting and active K channels in the squid axon membrane. In : Cephalopod Neurobiology.(Ed.Abbott,N.J., Williamson,R. and Maddok,L.), (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Yamagishi,S.et al.: Oxford University Press(in press). The effect of internal Ca^<2+> and Mg^<2+> on ion channels in the squid giant axons. In : Cephalopod Neurobiology.(Ed.Abbott,N., Williamson,R. and Maddock,L.M.), (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kishimoto,U.et al.: Oxford University Press(in press). Voltage dependence of the ouabain-sensitive current of the squid axon membrane. In : Cephalopod Neurobiology.(Ed.Abbott,N.J., Williamson,R. and Maddok,L.), (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Pichon,Y.et al.: (in press). Periaxonal K^+ accumulation and regulation in the squid. In : Cephalopod Neurobiology.(Ed.Abbott,N.J., Williamson,R. and Maddok,L.), (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Inoue,I.et al.: Elsevier Science Inc. Amsterdam(in press). Evolution of skeletal muscle excitation-contraction coupling. In : "Towards molecular Buiphysics of Ion Channels"., (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Inoue,I.: "Resting and active K channel in sguid axon membrane." in Cephalopod Neurobiology ed N.J.ABBOTT & R.Williamson.

    • Related Report
      1992 Annual Research Report
  • [Publications] Yamagishi,I.他: "Regulatory effects of intracellularly applied calcium and magnesium ions on ion channel activities in sguid giant axons." in Cephalopod Neurobiology ed N.J.ABBOTT & R.Williamson.

    • Related Report
      1992 Annual Research Report
  • [Publications] Kukita,F.他: "Kinetic analysis of the denaturation process of sodium channels in sguid giant axon by alcohols." J.Physiol.463. 523-543 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Brown,E.他: "Merphology and electrical properties of Schwann cells around the giant axon of the sguids Loligo forbesi & Loligo vulgaris." Proc.R.Soc.Lond.B. 243. 255-262 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Inoue,I.;ed N.J.ABBOTT & R.Williamson: "Resting and active K channel in squid axon membrane." Cephalopod Neurobiology.

    • Related Report
      1991 Annual Research Report
  • [Publications] Yamagishi,I.;ed N.J.ABBOTT & Williamson: "Regulatory effects of intercellularly applied calcium and magnesium ions on ion channel activities in squid giant axons." in Cephalopod Neurobiology.

    • Related Report
      1991 Annual Research Report
  • [Publications] Kukita,F.: "Kinetic analysis of the denaturation process of sodium channels in squid giant axon by alcohols." J.Physiol.

    • Related Report
      1991 Annual Research Report
  • [Publications] Brown,E.: "Morphology and electrical properties of Schwann cells around the giant axon of the squids Loligo forbesi & Loligo vulgaris." Proc.R.Soc.Lond.B. 243. 255-262 (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1991-03-31   Modified: 2016-04-21  

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